Efficient wheat germ agglutinin purification with a chitosan‐based affinity chromatographic matrix

Baieli, María Fernanda; Urtasun, Nicolás; Miranda, Magdalena; Cascone, Osvaldo; Wolman, Federico Javier

doi:10.1002/jssc.201100730

Cited by 15 publications

(17 citation statements)

References 17 publications

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“…The support matrix was synthesized as previously . A 2% chitosan solution was prepared by dissolution in acetic acid (2.0%).…”

Section: Methodsmentioning

confidence: 99%

“…The solutions were centrifuged at 17,212 g for 25 min and dripped on 2 M NaOH through a 15G needle . For the crosslinking reaction, a 250 m M epichlorohydrin solution was used as previously . The support matrix generated is named hereafter CH.…”

Section: Methodsmentioning

confidence: 99%

“…The support matrix was synthesized as previously. 25 A 2% chitosan solution was prepared by dissolution in acetic acid (2.0%). The solutions were centrifuged at 17,212 g for 25 min and dripped on 2 M NaOH through a 15G needle.…”

Section: Preparation Of the Support Matrixmentioning

confidence: 99%

“…Such matrix consisted of chitosan mini‐spheres with immobilized WGA. The process used to purify WGA from wheat germ extract using chitosan mini‐spheres was characterized and developed in a previous work …”

Section: Introductionmentioning

confidence: 99%

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Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey

Baieli

Urtasun

Martínez

et al. 2016

Biotechnology Progress

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Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nonglycosylated isoforms (aCMP A and aCMP B) and its different glycosylated forms (gCMP A and gCMP B). The most predominant carbohydrate of gCMP is N-acetylneuraminic acid (sialic acid). Here, we developed a CMP purification process based on the affinity of sialic acid for wheat germ agglutinin (WGA). After formation of chitosan beads and adsorption of WGA, the agglutinin was covalently attached with glutaraldehyde. Two matrices with different WGA density were assayed for CMP adsorption. Maximum adsorption capacities were calculated according to the Langmuir model from adsorption isotherms developed at pH 7.0, being 137.0 mg/g for the matrix with the best performance. In CMP reduction from whey, maximum removal percentage was 79% (specifically 33.7% of gCMP A and B, 75.8% of aCMP A, and 93.9% of aCMP B). The CMP was recovered as an aggregate with an overall yield of 64%. Therefore, the matrices developed are promising for CMP purification from cheese whey. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:171-180, 2017.

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“…The support matrix was synthesized as previously . A 2% chitosan solution was prepared by dissolution in acetic acid (2.0%).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Preparation Of the Support Matrixmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey

Baieli

Urtasun

Martínez

et al. 2016

Biotechnology Progress

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“…Due to its free amino and hydroxyl groups in its structure, chitosan is susceptible to undergo chemical modifications . Both chitin and chitosan present the advantage of being natural, biodegradable and environmentally safe biopolymers . Both polymers have many potential uses and applications in different fields that are gaining interest.…”

Section: Introductionmentioning

confidence: 99%

Sulfanilic acid‐modified chitosan mini‐spheres and their application for lysozyme purification from egg white

Hirsch

Baieli

Urtasun

et al. 2017

Biotechnology Progress

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A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico-chemically characterized by ss-NMR and ζ potential, and its chromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 ± 1.47 mg g while the dissociation constant was 0.074 ± 0.012 mg mL . The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP-HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:387-396, 2018.

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Chitosan/Polyvinylpyrrolidone/MCM‐41 Composite Hydrogel Films: Structural, Thermal, Surface, and Antibacterial Properties

et al. 2018

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The aim of this study is to characterize and investigate the effects of the Mobil Composition of Matter No. 41 (MCM-41) concentrations (0.5, 1, 3, 5, and 10 wt%) on structural, thermal, wettability, water content, oxygen (O 2 ) permeability, and swelling properties as well as morphological characteristics of chitosan/polyvinylpyrrolidone/MCM-41 (CH/PVP/MCM-41) composite hydrogel films. In addition, hydrolytic degradability, optic transmittance, and antibacterial properties of composite hydrogels films are determined as in vitro. With increasing of MCM-41 content, the composite hydrogels showed higher thermal stability, as expected. The morphologic properties of the composite hydrogel films are studied using scanning electron microscope (SEM) technique that provide evidence for good miscibility of CH, PVP, and MCM-41. The swelling and water-absorbing properties of the composite hydrogel films are significantly improved because of enhanced hydrophilic profile. Furthermore, the antimicrobial properties of the prepared composite hydrogel films are studied against Bacillus subtilis, Escherichia coli, and Candida albicans yeast. The results showed excellent antibacterial behavior of the composite hydrogel films. The study clearly demonstrates that the prepared CH/PVP/MCM-41 composite hydrogel films may be used as a promising candidate material in various fields such as drug delivery, wound healing material, and adsorbent materials.

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Efficient wheat germ agglutinin purification with a chitosan‐based affinity chromatographic matrix

Cited by 15 publications

References 17 publications

Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey

Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey

Sulfanilic acid‐modified chitosan mini‐spheres and their application for lysozyme purification from egg white

Chitosan/Polyvinylpyrrolidone/MCM‐41 Composite Hydrogel Films: Structural, Thermal, Surface, and Antibacterial Properties

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