EGF Enhances ADSCs Secretion via ERK and JNK Pathways

Li, Qiang; Li, Peihua; Hou, Dianju; Zhang, Aijun; Tao, Changbo; Li, Xueyang; Jin, Peisheng

doi:10.1007/s12013-013-9769-3

Cited by 18 publications

(12 citation statements)

References 29 publications

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“…The increase in VEGF and HGF we noted in presence of coculture media (Fig. 8) could likely be attributed to epidermal growth factor (EGF) presence in this endothelial media, since EGF has been previously shown to increase VEGF, HGF and stromal cell-derived factor-1 (SDF-1) secretion by ASCs [56]. Such an increase in VEGF expression mediated by EGM2 media could actually be beneficial overall, favoring the development CD31+ structures as well as increased platelet-derived growth factor (PDGF) levels in conditioned media [57].…”

Section: Discussionmentioning

confidence: 85%

Creating capillary networks within human engineered tissues: Impact of adipocytes and their secretory products

Aubin¹,

Vincent²,

Proulx³

et al. 2015

Acta Biomaterialia

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The development of tissue-engineered substitutes of substantial volume is closely associated with the need to ensure rapid vascularization upon grafting. Strategies promoting angiogenesis include the in vitro formation of capillary-like networks within engineered substitutes. We generated both connective and adipose tissues based on a cell sheet technology using human adipose-derived stromal cells. This study evaluates the morphology and extent of the capillary networks that developed upon seeding of human microvascular endothelial cells during tissue production. We posited that adipocyte presence/secretory products could modulate the resulting capillary network when compared to connective substitutes. Analyses including confocal imaging of CD31-labeled capillary-like networks indicated slight differences in their morphological appearance. However, the total volume occupied by the networks as well as the frequency distribution of the structure's volumes were similar between connective and adipose tissues. The average diameter of the capillary structures tended to be 20% higher in reconstructed adipose tissues. Quantification of pro-angiogenic molecules in conditioned media showed greater amounts of leptin (15×), angiopoietin-1 (3.4×) and HGF (1.7×) secreted from adipose than connective tissues at the time of endothelial cell seeding. However, this difference was attenuated during the following coculture period in endothelial cell-containing media, correlating with the minor differences noted between the networks. Taken together, we developed a protocol allowing reconstruction of both connective and adipose tissues featuring well-developed capillary networks in vitro. We performed a detailed characterization of the network architecture within engineered tissues that is relevant for graft assessment before implantation as well as for in vitro screening of angiogenic modulators using three-dimensional models.

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Section: Discussionmentioning

confidence: 85%

Creating capillary networks within human engineered tissues: Impact of adipocytes and their secretory products

Aubin¹,

Vincent²,

Proulx³

et al. 2015

Acta Biomaterialia

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“…Human ADSCs were isolated from subcutaneous adipose tissue samples obtained from the liposuction aspirates of patients undergoing cosmetic liposuction as previously described ( 19 ). Three healthy female patients with abdominal fat accumulation who underwent liposuction surgery at the Affiliated Hospital of Xuzhou Medical College (Xuzhou, China) were selected.…”

Section: Methodsmentioning

confidence: 99%

Stromal cell-derived factor-1 promotes human adipose tissue-derived stem cell survival and chronic wound healing

Guo

Chen

et al. 2016

Experimental and Therapeutic Medicine

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Adipose tissue-derived stem cells (ADSCs) hold great potential for the stem cell-based therapy of cutaneous wound healing. Stromal cell-derived factor-1 (SDF-1) activates CXC chemokine receptor (CXCR)4+ and CXCR7+ cells and plays an important role in wound healing. Increasing evidence suggests a critical role for SDF-1 in cell apoptosis and the survival of mesenchymal stem cells. However, the function of SDF-1 in the apoptosis and wound healing ability of ADSCs is not well understood. The aim of this study was to analyze the effect of SDF-1 on the apoptosis and therapeutic effect of ADSCs in cutaneous chronic wounds in vitro and in vivos. By flow cytometric analysis, it was found that hypoxia and serum free promoted the apoptosis of ADSCs. When pretreated with SDF-1, the apoptosis of ADSCs induced by hypoxia and serum depletion was partly recovered. Furthermore, in vivo experiments established that the post-implantation cell survival and chronic wound healing ability of ADSCs were increased following pretreatment with SDF-1 in a diabetic mouse model of chronic wound healing. To explore the potential mechanism underlying the effect of SDF-1 on ADSC apoptosis, western blot analysis was employed and the results indicate that SDF-1 may protect against cell apoptosis in hypoxic and serum-free conditions through activation of the caspase signaling pathway in ADSCs. This study provides evidence that SDF-1 pretreatment can increase the therapeutic effect of ADSCs in cutaneous chronic wounds in vitro and in vivo.

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“…For example, EGF treatment promoted the proliferation and maintained the multipotency of continuously cultured ASCs via activating the STAT-signaling pathway in vitro [15]. EGF increased the secretion of vascular endothelial growth factor, hepatocyte growth factor, and stromal-derived factor-1 via the ERK and JNK pathways in ASCs [16]. Epiregulin promotes the migration and chemotactic abilities of ASCs via the MAPK pathways [17].…”

Section: Introductionmentioning

confidence: 99%

HB-EGF Improves the Hair Regenerative Potential of Adipose-Derived Stem Cells via ROS Generation and Hck Phosphorylation

Choi

Kim

et al. 2019

IJMS

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Although adipose-derived stem cells (ASCs) have hair regenerative potential, their hair inductive capabilities are limited. The mitogenic and hair inductive effects of heparin binding-epidermal growth factor-like growth factor (HB-EGF) on ASCs were investigated in this study and the underlying mechanism of stimulation was examined. Cell growth, migration, and self-renewal assays, as well as quantitative polymerase chain reactions and immunostaining, were carried out. Telogen-to-anagen transition and organ culture using vibrissa follicles were also conducted. HB-EGF significantly increased ASC motility, including cell proliferation, migration, and self-renewal activity. The preconditioning of ASCs with HB-EGF induced telogen-to-anagen transition more rapidly in vivo, and injected PKH26-ASCs survived for longer periods of time. Conditioned medium obtained from HB-EGF-treated ASCs promoted hair growth in vivo, upregulating growth factors. In particular, thrombopoietin (THPO) also induced hair growth in vivo, stimulating dermal papilla cells (DPCs). Reactive oxygen species (ROS) appeared to play a key role in ASC stimulation as the inhibition of ROS generation and NOX4 knockout attenuated ASC stimulation and THPO upregulation by HB-EGF. In addition, the Hck phosphorylation pathway mediated the stimulation of ASCs by HB-EGF. In summary, HB-EGF increased the motility and paracrine effects of ASCs releasing THPO growth factor and THPO promoted hair growth-stimulating DPCs. ROS generation and Hck phosphorylation are key factors in HB-EGF-induced ASC stimulation. Therefore, combination therapy involving HB-EGF and ASCs may provide a novel solution for hair-loss treatment.

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EGF Enhances ADSCs Secretion via ERK and JNK Pathways

Cited by 18 publications

References 29 publications

Creating capillary networks within human engineered tissues: Impact of adipocytes and their secretory products

Creating capillary networks within human engineered tissues: Impact of adipocytes and their secretory products

Stromal cell-derived factor-1 promotes human adipose tissue-derived stem cell survival and chronic wound healing

HB-EGF Improves the Hair Regenerative Potential of Adipose-Derived Stem Cells via ROS Generation and Hck Phosphorylation

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