EGF-induced MMP-9 expression is mediated by the JAK3/ERK pathway, but not by the JAK3/STAT-3 pathway in a SKBR3 breast cancer cell line

Macrophage inhibitory cytokine-1 transactivates ErbB family receptors via the activation of Src in SK-BR-3 human breast cancer cells

“…Elevated expression of MMP-9 is associated with increased metastatic potential in many cancer types, including breast cancers (32). Several studies have demonstrated that activation of ErbB family receptors induces MMP-9 expression and invasiveness of human breast cancer cells (33,34). Our results showed that MIC-1 can induce MMP-9 expression through Src-dependent activation of ErbB-family receptors in SK-BR-3 cells.…”

Janus kinases and Src family kinases in the regulation of EGF-induced vimentin expression in MDA-MB-468 breast cancer cells

“…This suggests the involvement of JAKs in EGF-mediated induction of vimentin through an additional mechanism that is independent of STAT3. A JAK3-ERK (rather than a JAK3-STAT3) dependent signaling pathway has been implicated in the EGF-mediated induction of matrix metalloproteinase-9 in SKBR3 breast cancer cells (Kim et al, 2009). Alternatively, regulation of vimentin may bypass downstream signaling pathways, and occur via direct transcriptional and/or epigenetic regulation in the nucleus by one or more members of the JAK family, as has been described for JAK1 and JAK2 (Lobie et al, 1996;Zouein et al, 2011).…”

Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells

“…Zymography was performed on 10% polyacrylamide gels that had been cast in the presence of gelatin as previously described (15). Briefly, samples (100 µl) were resuspended in a loading buffer and run on a 10% SDS-PAGE gel containing 0.5 mg/ml gelatin without prior denaturation.…”