Egg-sperm interaction in sturgeon: role of ovarian fluid

Kholodnyy, Vitaliy; Dzyuba, Borys; Gadêlha, Hermes; Cosson, Jacky; Boryshpolets, Sergii

doi:10.1007/s10695-020-00852-2

Cited by 5 publications

(2 citation statements)

References 48 publications

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“…OF modulates sperm velocity as a mechanism of cryptic female choice [ 3 ]. The biochemical composition of sturgeon OF, including ions, proteins, amino acids and sugars, supports and protects fish gametes against the harmful effects of low freshwater osmolality [ 4 ]. The pH of OF and the contents of potassium, sodium and calcium ions are similar to other fish species, but osmolarity varies between fish species.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon

Kodzik,

Ciereszko,

Szczepkowska

et al. 2024

BMC Genomics

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Background Sturgeon species are living fossils that exhibit unique reproductive characteristics, and elucidation of the molecular processes governing the formation and quality of sturgeon eggs is crucial. However, comprehensive data on the protein composition of sturgeon ovarian fluid (OF) and eggs and their functional significance are lacking. To address this knowledge gap, the aim of the present study was to conduct a comprehensive comparative proteomic analysis of Siberian sturgeon OF and eggs using liquid chromatography–mass spectrometry (LC–MS/MS). Results A total of 617 proteins were identified in OF, and 565 proteins were identified in eggs. A total of 772 proteins showed differential abundance. Among the differentially abundant proteins, 365 were more abundant in OFs, while 407 were more abundant in eggs. We identified 339 proteins unique to OFs and 287 proteins specific to eggs, and further investigated the top 10 most abundant proteins in each. The functional annotation of the OF proteins highlighted their predominant association with immune system processes, including the complement and coagulation cascade, neutrophil and leukocyte-mediated immunity, cholesterol metabolism, and regulation of the actin cytoskeleton. Analysis of egg proteins revealed enrichment in metabolic pathways, such as oxidative phosphorylation and fatty acid metabolism, and protein ubiquitination and translation. OF-specific proteins included extracellular matrix and secretory vesicles, and eggs were enriched in proteins localized to mitochondria and ribosome components. Conclusions This study presents the first comprehensive characterization of the protein composition of sturgeon OF and eggs and elucidates their distinct functional roles. These findings advance our understanding of sturgeon reproduction, OF-egg signaling and the origin of OF proteins. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier PXD044168 to ensure accessibility for further research.

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Section: Introductionmentioning

confidence: 99%

Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon

Kodzik,

Ciereszko,

Szczepkowska

et al. 2024

BMC Genomics

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“…In contrast to the mechanisms involved in motility activation, the roles of ions and ion channels in the maintenance of the velocity and swimming behavior of post activated fish spermatozoa are much less known. Such mechanisms are nevertheless hypothesized to play important roles in physical and chemoattractant signaling pathways that guide and orient the spermatozoa towards the egg [ 16 , 17 , 18 , 19 ]. For example, a recent study in zebrafish suggests that the functional interaction of CNGK and Hcnl1 channels, both located in the spermatozoon head, may be crucial for successful navigation to the egg micropyle [ 5 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Role of Ion Channels in the Maintenance of Sperm Motility and Swimming Behavior in a Marine Teleost

Castro-Arnau

Chauvigné

Cerdà

2022

IJMS

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In oviparous marine fishes, the hyperosmotic induction of sperm motility in seawater (SW) is well established, however, the potential function of ion channels in the maintenance of post activated spermatozoon swimming performance remains largely unknown. Here, we investigated the influence of ion channels on the spermatozoon swimming parameters using the gilthead seabream (Sparus aurata) as a model for modern marine teleosts. Our data show that the SW-induced activation of seabream sperm motility requires three concomitant processes, the hyperosmotic shock, an ion-flux independent increase of the intracellular concentration of Ca2+ ([Ca2+]i), but not of [K+]i or [Na+]i, and the alkalization of the cytosol. The combination of all three processes is obligatory to trigger flagellar beating. However, the time-course monitoring of sperm motion kinetics and changes in the [Ca2+]i, [K+]i and [Na+]i in SW or in non-ionic activation media, showed that the post activated maintenance of spermatozoa motility is dependent on extracellular Ca2+ and K+. A meta-analysis of a seabream sperm transcriptome uncovered the expression of multiple ion channels, some of which were immunolocalized in the head and/or tail of the spermatozoon. Selective pharmacological inhibition of these ion channel families impaired the long-term motility, progressivity, and velocity of SW-activated spermatozoa. The data further revealed that some antagonists of K+-selective or Ca2+-selective channels, as well as of stretch-activated and mechanosensitive channels, altered the trajectory of spermatozoa, suggesting that these ion channels are likely involved in the control of the swimming pattern of the post activated spermatozoon. These combined findings provide new insight into the signaling pathways regulating spermatozoon activation and swimming performance in marine fishes.

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