Egg yolk plasma can replace egg yolk in stallion freezing extenders

Pillet, Elodie; Duchamp, Guy; Batellier, Florence; Beaumal, Valérie; Anton, Marc; Desherces, S.; Schmitt, Éric; Magistrini, Michèle

doi:10.1016/j.theriogenology.2010.07.015

Cited by 81 publications

(49 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Semen was collected on a regular basis (three times a week) with a closed artificial vagina (INRA model). After collection, raw semen was filtered through gauze to remove the gel fraction of the ejaculate and immediately frozen in INRA Freeze extender according to the procedure described by Pillet et al [35]. The straws were stored in liquid nitrogen until used.…”

Section: Donkey Semen Collection Freezing and Evaluationmentioning

confidence: 99%

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

et al. 2016

View full text Add to dashboard Cite

Section: Donkey Semen Collection Freezing and Evaluationmentioning

confidence: 99%

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

et al. 2016

View full text Add to dashboard Cite

“…Sin embargo, se ha descrito que la suplementación con YH genera riesgo microbiológico, alteraciones de la fosforilación oxidativa y la movilidad de los espermatozoides; así como produce complicaciones para realizar investigaciones bioquí-micas, metabólicas y microscópicas del semen (Wall y Foote, 1999;Moussa et al, 2002). Debido a esto, se ha recurrido a la centrifugación de la YH con la finalidad de eliminar algunos de sus componentes y conservar principalmente las LDL, mediante la producción de yema de huevo centrifugada (YHC) y plasma de yema de huevo, las cuales han permitido mejorar la congelabilidad del semen equino (Pillet et al, 2011;Nouri et al, 2013).…”

Section: Introductionunclassified

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

2017

View full text Add to dashboard Cite

Palabras clave adicionalesCalidad seminal. Crioprotectores. Plasma seminal. Yema de huevo. Criopreservación. resUMenLa congelación de semen de asno permite almacenar material genético por un periodo ilimitado de tiempo, por lo que puede ser empleado para generar bancos de germoplasma o para la cría de mulares y asnos. Esta investigación tuvo como objetivo evaluar alternativas de suplementación de un diluyente para la congelación de semen de asno. Se colectó el semen de cinco asnos criollos colombianos, dos eyaculados por animal (n= 10) y luego se congeló en diluyente Equiplus® suplementado con 3% de glicerol (GLY) ó 5% de dimetilformamida (DMF), 2% ó 5% de yema de huevo centrifugada (YHC) y 10% ó 20% de plasma seminal (PS). Se realizó la evaluación post-descongelación de la calidad seminal y de la peroxidación lipídica (PL). Para el análisis estadístico se ajustaron modelos mixtos, se realizó un análisis de regresión y comparaciones de medias por la prueba de Tukey. El semen congelado con 5% de DMF, 5% de YHC y 10% de PS presentó valores superiores de movilidad, vitalidad e integridad de membrana (p<0,05). La PL fue menor en diluyentes con 5% de YHC (p<0,05). La suplementación del diluyente de congelación con una combinación de 5% de DMF, 5% de YHC y 10% de PS permite mejorar la calidad post-descongelación del semen de asno. Effect of extender supplementation on the post-thaw semen quality of donkey sUMMarY Freezing of donkey semen allows storing genetic material for an unlimited period of time, which can be used to generate germoplasm banks or for breeding of mules or asses. This research aimed to evaluate supplementation alternatives for freezing donkey semen. Semen from five Colombian creole donkeys (n= 10) was collected and then frozen in Equiplus® extender supplemented with 3% of glycerol (GLY) or 5% of dimethylformamide (DMF), 2% or 5% of centrifuged egg yolk (CEY) and 10% or 20% of seminal plasma (SP). Post-thaw evaluation of semen quality and lipid peroxidation (LP) was conducted. For statistical analysis mixed models were adjusted, regression analysis and comparison of means by the Tukey test were performed. Frozen semen with DMF, 5% CEY and 10% SP presented superior results of motility and membrane integrity (p<0.05). The LP was lower in extenders with 5% CEY (p<0.05). Supplementation of freezing extender with the combination of 5% DMF, 5% CEY and 10% SP allows to improve the post-thaw quality of asinine semen.additional keYwords

show abstract

“…Two pathways may result in the formation of LPO's; the enzymatic membrane system using NAD(P)H as a substrate and the mitochondrial electron transport chain. The main source of oxidative stress for spermatozoa is the mitochondria [60]. ROS production is increased in sperm mitochondria due to freezing and thawing, while an osmotic mechanism may increase mitochondrial membrane permeability, thus activating apoptosis.…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

Cryoprotectants & Cryopreservation of Equine Semen: A Review of Industry Cryoprotectants and the Effects of Cryopreservation on Equine Semen Membranes

Prien¹

2016

JDVAR

View full text Add to dashboard Cite

show abstract

Egg yolk plasma can replace egg yolk in stallion freezing extenders

Cited by 81 publications

References 37 publications

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

Cryoprotectants & Cryopreservation of Equine Semen: A Review of Industry Cryoprotectants and the Effects of Cryopreservation on Equine Semen Membranes

Contact Info

Product

Resources

About