eIF4A3 Promotes RNA Viruses’ Replication by Inhibiting Innate Immune Responses

Gao, Qingxia; Jiang, Meijun; Zhao, Yi; Li, Guoli; Yang, Cha; Ren, Caiyue; Jin, Meilin; Chen, Huanchun; Zhou, Hongbo

doi:10.1128/jvi.01513-22

Cited by 6 publications

(2 citation statements)

References 42 publications

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“…20 These proteins also play a crucial role in the immune system’s response to viral infections by regulating viral RNA stability and translation. 21 Considering that BV increases susceptibility to sexually transmitted infections, including viral infections, these findings further support the potential involvement of RBPs in the immune response within the reproductive tract. Twenty five proteins related to the male reproductive system also were found to be upregulated.…”

Section: Resultsmentioning

confidence: 53%

Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips

Gutzeit,

Gulati,

Izadifar

et al. 2023

Preprint

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BackgroundThe cervicovaginal mucus that coats the upper surface of the vaginal epithelium is thought to serve as a selective barrier that helps to clear pathogens, however, its role in modulating the physiology and pathophysiology of the human vagina is poorly understood. Bacterial vaginosis (BV), a common disease of the female reproductive tract that increases susceptibility to sexually transmitted infections, pelvic inflammatory disease, infertility, preterm birth, and both maternal and neonatal infections is characterized by the presence of a wide array of strict and facultative anaerobes, often includingGardnerella vaginalis.ObjectiveTo assess the role of cervical mucus in preventing dysbiosis-associated complications and preserving vaginal health.Study DesignTo better understand the role of cervicovaginal mucus in vaginal health, we used human organ-on-a-chip (Organ Chip) microfluidic culture technology to analyze the effects of cervical mucus produced in a human Cervix Chip when transferred to a human Vagina Chip BV model. Both chips are lined by primary human organ-specific (cervical or vaginal) epithelium interfaced with organ-specific stromal fibroblasts.ResultsOur data show that mucus-containing effluents from Cervix Chips protect Vagina Chips from inflammation and epithelial cell injury caused by co-culture with dysbiotic microbiome containingG. vaginalis. Proteomic analysis of proteins produced by the Vagina Chip following treatment with the Cervix Chip mucus also revealed a collection of differentially abundant proteins that may contribute to the vaginal response to dysbiotic microbiome, which could represent potential diagnostic biomarkers or therapeutic targets for management of BV.ConclusionsThis study highlights the importance of cervical mucus in control of human vaginal physiology and pathophysiology, and demonstrates the potential value of Organ Chip technology for studies focused on health and diseases of the female reproductive tract.

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Section: Resultsmentioning

confidence: 53%

Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips

Gutzeit,

Gulati,

Izadifar

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…20 These proteins also play a crucial role in immune system response to viral infections by regulating viral RNA stability and translation. 21 Considering that BV increases susceptibility to sexually transmitted infections, including viral infections, these ndings further support the potential involvement of RBPs in the immune response within the reproductive tract. Twenty-ve proteins related to the male reproductive system were also found to be upregulated.…”

Section: Cervix Chip Mucus Alters the Vaginal Secretomementioning

confidence: 54%

Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips

Gutzeit,

GULATI,

IZADIFAR

et al. 2024

Preprint

View full text Add to dashboard Cite

Background The cervicovaginal mucus which coats the upper surface of the vaginal epithelium is thought to serve as a selective barrier that helps to clear pathogens, however, its role in modulating the physiology and pathophysiology of the human vagina is poorly understood. Bacterial vaginosis (BV), a common disease of the female reproductive tract that increases susceptibility to sexually transmitted infections, pelvic inflammatory disease, infertility, preterm birth, and both maternal and neonatal infections is characterized by the presence of a wide array of strict and facultative anaerobes, often including Gardnerella vaginalis. Objective To assess the role of cervical mucus in preventing dysbiosis-associated complications and preserving vaginal health. Study Design: To better understand the role of cervicovaginal mucus in vaginal health, we used human organ-on-a-chip (Organ Chip) microfluidic culture technology to analyze the effects of cervical mucus produced in a human Cervix Chip and then transferred to a human Vagina Chip BV model. Both chips are lined by primary human organ-specific (cervical or vaginal) epithelium interfaced with organ-specific stromal fibroblasts. Results Our data show that mucus-containing effluents from Cervix Chips protect Vagina Chips from inflammation and epithelial cell injury caused by co-culture with a dysbiotic microbiome containing G. vaginalis. Proteomic analysis of proteins produced by the Vagina Chip following treatment with the Cervix Chip mucus also revealed a collection of differentially abundant proteins that may contribute to the vaginal response to a dysbiotic microbiome, which could represent potential diagnostic biomarkers or therapeutic targets for the management of BV. Conclusions This study highlights the importance of cervical mucus in controlling human vaginal physiology and pathophysiology, and demonstrates the potential value of Organ Chip technology for studies focused on the health and diseases of the female reproductive tract.

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On the interface of aging, cancer, and neurodegeneration with SIRT6 and L1 retrotransposon protein interaction network

Nahálková

2024

Ageing Research Reviews

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eIF4A3 Promotes RNA Viruses’ Replication by Inhibiting Innate Immune Responses

Abstract: Production of type I IFN is pivotal for the cellular antiviral immunity. Virus infection leads to the activation of transcription factor IRF3 and subsequent production of type I IFN to eliminate viral infection.

Cited by 6 publications

References 42 publications

Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips

Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips

Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips

On the interface of aging, cancer, and neurodegeneration with SIRT6 and L1 retrotransposon protein interaction network

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