Eimeria collieie n. sp. (Apicomplexa:Eimeriidae) from the western long-necked turtle (Chelodina colliei)

Abstract

• Morphological characterisation:Unique from all other reported Eimeria species.• Molecular characterisation at 18S, 28S rRNA and COI loci: Related to Eimeria arnyi. ). Molecular analysis was conducted at three loci; the 18S and 28S ribosomal RNA (rRNA) and the mitochrondial cytochrome oxidase gene (COI). At the 18S rRNA locus, E. collieie n. sp. shared 96.4% and 98.3% genetic similarity to E. ranae (GenBank accession number: EU717219) and E. arnyi (AY613853). At 28S rRNA locus, E. collieie n. sp. shared 91.6% genetic similarity to E. papillata (GenBank accession number: GU593706) and phylogenetic analysis at this locus placed E. collieie n. sp. in a separate clade. At the COI locus, E. collieie n. sp. shared 92.7% genetic similarity to Eimeria setonicis (GenBank accession number: KF225638) from a quokka (Setonix brachyurus) in Western Australia. However reptile-derived sequences were not available for the 28S rRNA and the COI loci. Based on morphological and molecular data, this isolate is a new species of coccidian parasite that, to date, has only been found in western long-necked turtles.

A New Coccidian (Apicomplexa: Eimeriidae) in the Critically Endangered Central American River Turtle (Dermatemys Mawii) in Belize

“…accurately (Tenter et al, 2002). Yang et al (2015) conducted molecular analysis at 3 loci: the 18S and 28S rRNA, and the mitochondrial cytochrome oxidase gene (COI) in reporting a new eimerian, E. collieie in the Western long-necked turtle (Chelodina colliei), a species native to Australia. At the 18S rRNA locus, E. collieie shared 96.4% and 98.3% genetic similarity to Eimeria ranae (GenBank accession number: EU717219) and E. arnyi (AY613853), respectively.…”

Eimeria spp. and Tyzzeria perniciosa Allen, 1936 (Apicomplexa: Eimeriidae) from a Pacific black duck, Anas superciliosa Gmelin (Aves: Anseriformes), in western Australia

“…Five morphologically similar oöcysts were isolated for a bulk DNA extraction with the method described by Yang et al. (2015) .…”