Eimeria maxima recombinant Gam82 gametocyte antigen vaccine protects against coccidiosis and augments humoral and cell-mediated immunity

Jang, Seung I.; Lillehoj, Hyun S.; Lee, Sung Hyen; Lee, Kyung-Woo; Park, Myeong Seon; Cha, Sung-Rok; Lillehoj, Erik P.; Subramanian, B. Mohana; Sriraman, Rajan; Srinivasan, V.A.

doi:10.1016/j.vaccine.2010.02.011

Cited by 54 publications

(39 citation statements)

References 24 publications

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“…Interestingly, the 56 and 230 kDa antigens also induced the production of IgY antibodies which are transferrable to the embryo via egg yolk (Belli et al 2002) and can provide protective immunity against coccidiosis following challenge infection (Lee et al 2009). An 82 kDa recombinant protein associated with gametocyte of E. maxima has could induce protective intestinal immunity resulting in decreased oocysts shedding and reduced gut pathology (Jang et al 2010) associated with increase in the body weights of chickens (Anwar et al 2008).…”

Section: Recombinant Vaccinementioning

confidence: 99%

Control of poultry coccidiosis: changing trends

Tewari

Maharana

2011

J Parasit Dis

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Coccidiosis is the most important protozoan disease affecting the poultry industry worldwide. Control of poultry coccidiosis is presently based on managerial skills and the use of prophylactic coccidiostatic drugs. With the emergence of drug resistant Eimeria strains, emphasis has been laid on development and use of safer vaccines; some of them have been commercialized successfully. The present review deals with the various factors responsible for the development of clinical coccidiosis in poultry as well as an overview of the currently available inducers and boosters of immunity against coccidiosis. There are three groups of vaccines currently available against coccidiosis which can be distinguished on the basis of characteristics of the Eimeria species included in the respective products, viz. vaccines based on live virulent strains, vaccines based on live attenuated strains, and vaccines based on live strains that are relatively tolerant to the ionophore compounds. The latter vaccine combines the early chemotherapeutic effect of ionophores with the late prophylactic effect of vaccination. Although in the near future more varieties of oocyst based live vaccines are expected, identification of selective coccidian-specific immunoprotective molecules is likely to get more attention to facilitate the sustainable control of poultry coccidiosis.

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Section: Recombinant Vaccinementioning

confidence: 99%

Control of poultry coccidiosis: changing trends

Tewari

Maharana

2011

J Parasit Dis

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“…21,27,28 IgA is probably the most important isotype involved in coccidial infections. Secretory IgA has been detected in bile and intestinal washings of E. tenella-infected birds as early as 7 days postinoculation.…”

Section: 26mentioning

confidence: 99%

“…Several antigens, both in their native form and as recombinant proteins, have been successfully employed in various studies. 27,[59][60][61][62][63][64][65][66] Their efficacy in terms of stimulating a robust immune response is remarkable, as they are involved in establishing a well-defined host-parasite relationship. Even though recombinant vaccines have not been successful commercially, efforts are being made to decipher hostparasite interactions, leading to much more meaningful approaches for recombinant vaccine development.…”

Section: The Role Of Recombinant Vectored Vaccinesmentioning

confidence: 99%

“…Several studies report the use of E. coli and Salmonella to deliver Eimeria epitopes. 27,70,71 Some other researchers have been able to use attenuated Salmonella successfully as a vaccine delivery tool. 61,62 Eimeria spp., being intestinal parasites, have a predisposition to the gut mucosa, and therefore, mucosal delivery of antigens is the preferred target for optimum vaccine efficacy.…”

Section: The Role Of Recombinant Vectored Vaccinesmentioning

confidence: 99%

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Coccidiosis: recent advancements in the immunobiology of Eimeria species, preventive measures, and the importance of vaccination as a control tool against these Apicomplexan parasites

Shivaramaiah¹,

Barta²,

Hernández-Velasco³

et al. 2014

VMRR

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Coccidiosis, caused by parasites of the genus Eimeria, is probably the most expensive parasitic disease of poultry. Species of Eimeria are ubiquitous where poultry are raised and are known to cause drastic reductions in performance and induce mortality, thereby affecting the overall health status of poultry. Chemotherapy has been the predominant form of disease control for many years, even though vaccination is steadily gaining importance as a feasible control method. The objective of this review is to highlight recent advancements in understanding the role of host immunity against coccidiosis. In addition, pros and cons associated with chemotherapy and the role of vaccination as an increasingly popular disease control method are discussed. Finally, the role played by recombinant vaccines as a potential vaccination tool is highlighted. With interest growing rapidly in understanding host-parasite biology, recent developments in designing recombinant vaccines and potential epitopes that have shown promise are mentioned.

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“…The three species that most commonly affect the poultry industry are: E. tenella, E. maxima and E. acervulina (CARDOZO;YAMAMURA, 2006;McDOUGALD, 2007). Among these, E. maxima is considered to be the one with the highest pathogenicity (SHARMAN et al, 2010), causing lesions that may compromise the absorption surface of birds' digestive tract (JANG et al, 2010;JENKINS et al, 2009). In addition, it may also favor establishment of other diseases, since the damaged intestinal tissues and the functional changes to the intestinal tract that it causes may break down the natural defense barriers and allow colonization by various pathogenic agents.…”

Section: Introductionmentioning

confidence: 99%

Metabolic alterations in broiler chickens experimentally infected with sporulated oocysts of Eimeria maxima

Freitas

2014

Rev. Bras. Parasitol. Vet.

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Metabolic and morphometric alterations of the duodenal villi caused by parasitism of chickens by Eimeria maxima were evaluated, using 100 male Cobb birds, randomly distributed into two groups (control and infected). The infected group was inoculated with 0.5 ml of a solution containing 5×10 3 sporulated oocysts of Eimeria maxima. Ten birds per sample were sacrificed on the 6 th , 11 th , 22 nd and 41 st days post-infection (dpi). In order to evaluate the alterations, samples of duodenum, jejunum and ileum fragments were collected after necropsy for histological analysis. Villus biometry was determined by means of a slide graduated in microns that was attached to a binocular microscope. To evaluate the biochemical data, 5 ml of blood were sampled from the birds before sacrifice. The statistical analyses were performed using the GraphPad 5 statistical software for Windows. Tukey's multiple comparison test (p < 0.05) was performed for the different dpi's and the unpaired t test for the difference between the groups. Infection by E. maxima causes both qualitative and quantitative alterations to the structure of the intestinal villi, thereby interfering with the absorption of nutrients such as calcium, phosphorus, magnesium, protein and lipids, with consequent reductions in the birds' weights.Keywords: Eimeria maxima, metabolism, experimental infection. ResumoForam avaliadas alterações metabólicas e morfométricas das vilosidades intestinais causadas pelo parasitismo de frangos por Eimeria maxima, sendo utilizadas 100 aves da linhagem Coob, machos, distribuídos aleatoriamente em dois grupos experimentais: grupo controle, inoculado com 0,5 ml de água destilada; grupo infectado, inoculado com 0,5ml de solução contendo 5×10 3 oocistos esporulados de Eimeria maxima. Foram sacrificadas 10 aves por coleta no 0, 6, 11, 22 e 41 dias pós-infecção. Para avaliar as alterações foram retiradas, após necropsia, amostras de fragmentos do duodeno, jejuno e íleo para análise histológica. A determinação da biometria de vilosidades foi realizada por meio de lâmina milimetrada acoplada a um microscópio binocular. Para avaliação dos dados bioquímicos foram coletados 5 ml de sangue das aves antes da eutanásia. As análises estatísticas foram realizadas, utilizando-se o programa estatístico Graphpad Prism. 5 -Windows e realizado o teste de comparações múltiplas de Tukey ( p < 0,05) para os diferentes dpi's e o Teste T não Pareado para diferença entre os grupos. A infecção por E. maxima provoca alterações qualitativas e quantitativas das vilosidades intestinais, interferindo na absorção de nutrientes, como cálcio, fósforo, magnésio, proteínas e lipídios, com consequente redução no peso das aves.Palavras-chave: Eimeria maxima, metabolismo, infecção experimental.

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Eimeria maxima recombinant Gam82 gametocyte antigen vaccine protects against coccidiosis and augments humoral and cell-mediated immunity

Cited by 54 publications

References 24 publications

Control of poultry coccidiosis: changing trends

Control of poultry coccidiosis: changing trends

Coccidiosis: recent advancements in the immunobiology of Eimeria species, preventive measures, and the importance of vaccination as a control tool against these Apicomplexan parasites

Metabolic alterations in broiler chickens experimentally infected with sporulated oocysts of Eimeria maxima

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