El lavado de la aguja aumenta la rentabilidad diagnóstica de la punción-aspiración con aguja fina de tiroides

Álvarez, Manuel Penín; Fraile, P. San Miguel; Cruz, Inés Seoane; Sarmiento, Rosa Cunqueiro; Carballeira, Regina Palmeiro; Cano, Reyes Luna

doi:10.1016/j.endonu.2012.09.015

Cited by 10 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since the approval of ThinPrep slides by the US FDA for processing non-gynecological material in 1991, a growing number of laboratories are processing FNA, bronchial brushing, and effusion specimens with this method [19, 20]. In our laboratory, ThinPrep specimens are the only available cytology material for bronchial brushing and imaging-guided FNA specimens.…”

Section: Discussionmentioning

confidence: 99%

Application of Ventana immunocytochemical analysis on ThinPrep cytology slides for detection of ALK rearrangement in patients with advanced non–small-cell lung cancer

Guo¹,

Jia

Zhao

et al. 2018

BMC Cancer

View full text Add to dashboard Cite

BackgroundVentana ALK (D5F3) screening of anaplastic lymphoma kinase (ALK) gene rearrangement in tissue specimens has been approved by US FDA (Food and Drug Administration) to select treatment for non–small-cell lung carcinoma (NSCLC). However, tumor tissues are often not readily obtainable, and cytology specimens and may be the only tumor material available for diagnosis and molecular marker analysis. In this study, we evaluated the feasibility of ALK immunocytochemistry (ICC) on ThinPrep slides and determined a suitable scoring system for interpretation of the results.MethodsOne hundred twenty-one fine-needle aspirate (FNA) specimens from metastatic lesions of NSCLC were analyzed. ALK rearrangement was detected on ThinPrep cytology slides using the Ventana immunocytochemistry ALK-D5F3 system, which adopts two scoring systems for interpretation of the ICC results. The results were subsequently confirmed by reverse transcription polymerase chain reaction (RT-PCR) analysis and fluorescence in situ hybridization (FISH).ResultsAmong the 121 ICC specimens, 16 that were considered ALK-positive by either scoring system were referred for PCR analysis. Among the ALK ICC-negative cases, 33 had correlated FISH ALK results. A total of 49 specimens that exhibited either a positive or negative ICC result with a correlated ALK status were analyzed statistically. ICC results showed a high concordance rate with the results of PCR/FISH analysis. The sensitivity and specificity of ALK ICC by the binary scoring algorithm were 68.75 and 96.97%, respectively. These values increased to 93.75 and 96.97%, respectively, when interpreted by the semiquantified interpretation system.ConclusionsALK ICC analysis on ThinPrep slides is a reliable ALK testing method, and the semiquantified interpretation system on cytology specimens is recommended rather than the binary scoring algorithm on tissue specimens.

show abstract

Section: Discussionmentioning

confidence: 99%

Application of Ventana immunocytochemical analysis on ThinPrep cytology slides for detection of ALK rearrangement in patients with advanced non–small-cell lung cancer

Guo¹,

Jia

Zhao

et al. 2018

BMC Cancer

View full text Add to dashboard Cite

show abstract

“…Studies have evaluated the utility of TP in thyroid FNA, but the results have been conflicting. Some investigators reported TP to be inferior to CP, whereas others reported TP to be equivalent and even superior to CP [1,2,3,4]. We retrospectively reviewed our patients treated with thyroid FNA using the TP preparation.…”

Section: Discussionmentioning

confidence: 99%

“…The application of TP in combination with conventional smears has been shown to reduce the nondiagnostic rate [1,2]. However, the efficacy of TP alone in triaging thyroid nodules is still controversial [3,4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Factors Influencing the Reliability of Thyroid Fine-Needle Aspiration: Analysis of Thyroid Nodule Size, Guidance Mode for Aspiration and Preparation Method

H¹,

Zhang²,

Zhao³

et al. 2015

Acta Cytologica

View full text Add to dashboard Cite

Objective: We aimed to clarify the influence of ThinPrep preparation, nodule size and guidance mode on the accuracy of thyroid fine-needle aspiration (FNA). Methods: A total of 1,240 thyroid FNAs were reviewed and 489 cases with histological correlations were enrolled in this study. Results: Of the 489 total cases examined, 101 were processed with both ThinPrep and conventional preparation and 388 entirely with ThinPrep. The overall nondiagnostic rate, sensitivity and accuracy of FNA were 2.0, 91.0 and 89.4%, respectively. The cases with a preoperative ultrasound (n = 469) were grouped according to nodule size. The nondiagnostic rate, sensitivity and accuracy of FNA did not differ significantly with nodule size (p1 = 0.339, p2 = 0.179, p3 = 0.119). A total of 101 resections were performed with palpation-guided FNA and 388 were performed with ultrasound-guided FNA. The nondiagnostic rates, sensitivity and accuracy of FNA were similar in these two groups. Conclusions: The ThinPrep technique is a valid method for thyroid FNA and is effective for thyroid nodules ≥0.5 cm. The reliability of FNA results is not reduced with larger nodules. The use of palpation-guided FNA for palpable solid nodules is also effective.

show abstract

“…La PAAF como método de diagnóstico, se conoce desde el siglo XIX, cuando Kun en 1847 describió la técnica de aspiración de material celular para estudio y los cirujanos londinenses Stanley y Earle del St Bartholomeuw´s Hospital, obtuvieron muestras de una gran masa hepática para su estudio citológico mediante agujas finas. Posteriormente Sir James Paget defendió el uso de la aspiración de material celular como procedimiento investigativo en 1853, décadas después Richard (1863) y Leyden (1883) también se refirieron al tema 5 .…”

Section: Desarrollounclassified

Punción aspiración con aguja fina de nódulos tiroideos

et al. 2017

View full text Add to dashboard Cite

La punción aspirativa con aguja fina es una técnica muy habitual en las últimas décadas, que interpretada por un citólogo experto proporciona una información muy valiosa, se basa en un método simple, mínimamente invasivo y preciso para diagnosticar masas superficiales tales como los nódulos tiroideos; definidos por la Asociación Tiroidea Americana como discretas lesiones dentro de la glándula tiroidea imagenológicamente distintas del parénquima tiroideo. En los nódulos fríos indica si la citología es benigna, dudosa o maligna, con escasas probabilidades de error. En los carcinomas papilares su fiabilidad se acerca al 100% e igualmente en los carcinomas anaplásicos y carcinomas medulares; no así en los adenomas foliculares que son indiferenciables de los carcinomas, por lo cual el diagnóstico de proliferación folicular no es definitivo hasta después de la cirugía cuando esté el informe histopatológico de la biopsia por parafina, que sí es concluyente. En esta revisión bibliográfica se abordan los referentes teóricos más actuales sobre la importancia de la punción aspiración con aguja fina en el estudio de las lesiones nodulares de la glándula tiroides, tanto a nivel nacional como internacional, si se tiene en cuenta la tendencia al incremento del cáncer de tiroides en el Ecuador.

show abstract

El lavado de la aguja aumenta la rentabilidad diagnóstica de la punción-aspiración con aguja fina de tiroides

Cited by 10 publications

References 10 publications

Application of Ventana immunocytochemical analysis on ThinPrep cytology slides for detection of ALK rearrangement in patients with advanced non–small-cell lung cancer

Application of Ventana immunocytochemical analysis on ThinPrep cytology slides for detection of ALK rearrangement in patients with advanced non–small-cell lung cancer

Factors Influencing the Reliability of Thyroid Fine-Needle Aspiration: Analysis of Thyroid Nodule Size, Guidance Mode for Aspiration and Preparation Method

Punción aspiración con aguja fina de nódulos tiroideos

Contact Info

Product

Resources

About