Objective: to develop and validate the content of an instrument to monitor hemotherapy attention in liver transplant patients. Methods: methodological study in two stages: 1) Scoping review to identify relevant topics for the elaboration of the instrument; 2) Content validation with five specialists in two rounds, using the Delphi technique. Results: the final version of the instrument for hemotherapy care to liver transplant patients had four dimensions: Patient identification; Preoperative; Intraoperative; and Postoperative, to a total of 54 items. After the second round, all items had a content validation index of 0.8 or higher, and the instrument had a final score of 0.97. Conclusion: the instrument showed evidence of content validity, meaning it is a useful tool to monitor hemotherapy care for liver transplant patients. Contributions for the practice: the validation of this instrument will provide teams of transfusion and transplant centers with essential information to guide safe and efficient hemotherapy attention during the entirety of the liver transplant process.