Elastin and collagen IV double staining: A refined method to detect blood vessel invasion in breast cancer

Fujisawa, Masayoshi; Omori, Masako; Doihara, Hiroyoshi; Than, Ye Min; Swe, Hnin Wint Wint; Yoshimura, Teizo; Matsukawa, Akihiro

doi:10.1111/pin.12971

Cited by 6 publications

(11 citation statements)

References 42 publications

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“…Approximately 40% of patients with ER-positive breast cancer have defects in the DNA repair mechanism, which is an important factor contributing to endocrine resistance in breast cancer. A study revealed that compared to patients with low ERCC1 expression, (30,31). Multivariate regression analysis revealed that vascular tumor thrombus is an independent prognostic factor for patients with breast cancer.…”

Section: Discussionmentioning

confidence: 99%

“…This increases the risk of lymph node and distant metastasis in patients with breast cancer. Vascular tumor thrombus indicates poor prognosis in patients with breast cancer and can predict distant metastasis and local recurrence of tumors ( 30 , 31 ). Multivariate regression analysis revealed that vascular tumor thrombus is an independent prognostic factor for patients with breast cancer.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

ERCC1 is a potential biomarker for predicting prognosis, immunotherapy, chemotherapy efficacy, and expression validation in HER2 over-expressing breast cancer

Liao²,

Ma³

2022

Front. Oncol.

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ObjectiveTo investigate the relationship between Excision repair cross-complementation 1 (ERCC1) expression, clinicopathological features, and breast cancer prognosis in patients treated with trastuzumab. Further, we aim to explore the immune status of ERCC1 in breast cancer.MethodsThe data were retrieved from publicly available databases like the Cancer Genome Atlas, Therapeutically Applicable Research to Generate Effective Treatments, and the Genotype-Tissue Expression. The data was used to perform differential expression analyses between tumor and normal tissues in pan-cancers, immune-related analysis, homologous recombination deficiency (HRD), tumor mutation burden, and microsatellite instability. A total of 210 patients with HER2 over-expressing breast cancer from the Fourth Hospital of Hebei Medical University between January 2013 to December 2015 were enrolled in the study. Ten adjacent normal tissues were used to study the expression pattern of ERCC1 in normal tissues. Immunohistochemistry was performed to study ERCC1 expression and immune cell infiltration in different status of ERCC1 expression. Further, the correlation between ERCC1 expression, immune cell infiltration clinicopathological features, and the prognosis of patients with breast cancer was analyzed.ResultsThe immune analysis revealed a significant correlation between CD8+ T cell, CD4+ T cell, T helper cell, macrophages, mast cells, and ERCC1 expression. Spearman analysis show that ERCC1 expression is related to macrophages and T cells. A close correlation was observed between increased ERCC1 expression and high tumor immune dysfunction and exclusion (TIDE) score as well as HRD. The results revealed a significant correlation among ERCC1, chemotherapy and estrogen receptor (ER; P < 0.05) expression. Univariate survival analysis revealed a significant correlation (P < 0.05) between that ERCC1 and ER expression, blood vessel invasion, and disease-free survival (DFS). ERCC1 and ER expression, tumor size, blood vessel invasion, pathological type, and lymph node metastases significantly correlated (P < 0.05) with overall survival in patients. Multivariate regression analysis revealed that ERCC1 expression and chemotherapy were independent factors that influence DFS. ERCC1 expression and vascular tumor thrombus were independent influencing factors that influence OS.ConclusionA correlation was observed between high ERCC1 expression and poor patient prognosis. High ERCC1 expression also influences the efficacy of immunotherapy and chemotherapy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

ERCC1 is a potential biomarker for predicting prognosis, immunotherapy, chemotherapy efficacy, and expression validation in HER2 over-expressing breast cancer

Liao²,

Ma³

2022

Front. Oncol.

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“…H&E, D2-40, CD31, CD31 and collagen IV double staining were performed by the conventional immunohistochemistry method. Elastin and collagen IV double staining was performed as described previously [23]. Complete information on the antibodies and the staining procedures can be found in Supplementary Tables S1-S3.…”

Section: Tissue Staining By Immunohistochemistrymentioning

confidence: 99%

“…Histological analysis MVD, GMP, C-shaped, and excessively branched capillaries were investigated for each case. Lymphatic vessel invasion and blood vessel invasion were analyzed as described previously [23]. In the invasive tumor areas, the structure of the blood vessel can be identi ed by a brown-stained structure with or without lumen using CD31 single staining.…”

Section: Tissue Staining By Immunohistochemistrymentioning

confidence: 99%

The Specific Shapes of Capillaries are Associated with Worse Prognosis in Patients with Invasive Breast Cancer

Swe,

Fujisawa,

Ohara

et al. 2024

Preprint

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Purpose Angiogenesis is considered essential for tumor progression; however, whether histological counting of blood vessel numbers, expressed as microvessel density (MVD), can be a prognostic factor in breast cancer remains controversial. It has been suggested that the specific morphology of blood vessels such as glomeruloid microvascular proliferation (GMP) was associated with clinical parameters. Here, we aimed to clarify the significance of MVD with revised immunohistochemistry and to identify new blood vessel shapes that predict prognosis in breast cancer. Methods Four hundred and eleven primary breast cancer specimens were collected, and the sections were immunohistochemically stained with CD31 (single staining) and CD31 and Collagen IV (double staining). The prognosis of patients was examined according to MVD value, and the presence of GMP and other blood vessels with other specific shapes. Results With a sensitive and specific double immunostaining, high MVD value was associated even with better survival. The presence or absence of GMP was not related to patients’ prognosis. By contrast, patients with deep-curved capillaries surrounding tumor cell nests (C-shaped) or excessively branched capillaries near tumor cell nests showed a significantly poor prognosis. The presence of these capillaries was also correlated with clinicopathological parameters such as Ki-67 index and histological grade. Conclusion Even though MVD is widely used as a marker of angiogenesis, the complex morphology of blood vessels is significantly associated with clinical parameters. To estimate patients’ prognosis, the shape of blood vessels should also be taken into account.

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“…Detecting blood vessel invasion in breast cancer is of limited diagnostic usefulness due to the components of intraductal carcinomas mimicking blood vessel invasion. A study by Fujisawa et al used a combination stain of Victoria blue for elastin and IHC for collagen type IV [64]. This allowed the differentiation between the tissues of vascular origin, which would show reigned or lost collagen fibers, and the tissues of intraductal carcinoma, which would show a normal arrangement of the collagen in the basement membrane [64].…”

Section: Use Of Elastin Stains In Malignancies Other Than Colorectal ...mentioning

confidence: 99%

The Usefulness of Elastin Staining to Detect Vascular Invasion in Cancer

Gonzalez,

Bahmad,

Ocejo

et al. 2023

IJMS

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Tumor prognosis hinges on accurate cancer staging, a pivotal process influenced by the identification of lymphovascular invasion (LVI), i.e., blood vessel and lymphatic vessel invasion. Protocols by the College of American Pathologists (CAP) and the World Health Organization (WHO) have been established to assess LVI in various tumor types, including, but not limited to, breast cancer, colorectal cancer (CRC), pancreatic exocrine tumors, and thyroid carcinomas. The CAP refers to blood vessel invasion as “angioinvasion” (vascular invasion) to differentiate it from lymphatic vessel invasion (lymphatic invasion). For clarity, the latter terms will be used throughout this review. The presence of lymphatic and/or vascular invasion has emerged as a pivotal prognostic factor; therefore, its accurate identification is crucial not only for staging but also for providing the patient with an honest understanding of his/her prognosis. Given the prognostic importance of the correct identification of LVI, specific staining techniques are employed to distinguish lymphatic vessel invasion from angioinvasion and to differentiate true LVI from artifact. These encompass hematoxylin and eosin (H&E) staining, elastic staining, Factor VIII staining, Ulex europaeus I agglutinin staining, CD31, CD34, D2-40, ERG, and D2-40 (podoplanin) immunohistochemical (IHC) stains among others. Based on a review of numerous publications regarding the efficacy of various methods for LVI detection, elastin staining demonstrated superior accuracy and prognostic value, allowing for more targeted treatment strategies. The clinical significance of accurately detecting LVI cannot be overstated, as it is strongly linked to higher cancer-related mortality and an increased risk of tumor recurrence. This review aims to examine the existing literature on the use of elastin stains in the detection of vascular invasion among different types of tumors and its prognostic value.

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Elastin and collagen IV double staining: A refined method to detect blood vessel invasion in breast cancer

Cited by 6 publications

References 42 publications

ERCC1 is a potential biomarker for predicting prognosis, immunotherapy, chemotherapy efficacy, and expression validation in HER2 over-expressing breast cancer

ERCC1 is a potential biomarker for predicting prognosis, immunotherapy, chemotherapy efficacy, and expression validation in HER2 over-expressing breast cancer

The Specific Shapes of Capillaries are Associated with Worse Prognosis in Patients with Invasive Breast Cancer

The Usefulness of Elastin Staining to Detect Vascular Invasion in Cancer

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