Electric cell–substrate impedance sensing technique to monitor cellular behaviours of cancer cells

Pradhan, Rangadhar; Rajput, Shashi; Mandal, Mahitosh; Mitra, Analava; Das, Soumen

doi:10.1039/c3ra45090b

Cited by 18 publications

(14 citation statements)

References 33 publications

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“…3.10) software was used to fit the impedance data. Pradhan et al (Pradhan et al 2014 ) supplied the equivalent circuit for the impedance measurements explained in Fig. 5 .…”

Section: Methodsmentioning

confidence: 99%

Biosynthesis, characterization of PLGA coated folate-mediated multiple drug loaded copper oxide (CuO) nanoparticles and it’s cytotoxicity on nasopharyngeal cancer cell lines

Guo

Zhao

et al. 2020

AMB Expr

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Cytotoxicity of CuO nanoparticles (NPs) are an impediment in utilizing them as an effective nanocarriers of chemotherapeutic drugs for targeted drug delivery in nasopharyngeal cancer. In our current study, we have designed a two-step synthesis and coating of CuO NPs with different concentrations of PLGA (polylactide-co-glycolide) to reduce the cytotoxicity. This was further conjugated with folic acid to enhance targeting to specific tissue. The multiple drugs loaded in the NPs were two potent anticancer drugs doxorubicin and docetaxel. A complete characterization studies including micrographic analysis, zeta potential measurements, polydispersity index, Fourier transform infrared spectroscopy (FTIR), encapsulation and loading efficiencies, stability and in vitro release studies were done. Cytoxicity studies were done with MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, acridine orange/ethidium bromide and DAPI (4, 6-diamidino-2-phenylindole, dihydrochloride) staining procedures. Impediametric studies were also carried out to reinforce the reduction in cytotoxicity. Finally the cellular uptake of the NPs was seen. It was evident from the results that the multiple drugs loaded CuO NPs formed with PLGA coating were uniform, non-agglomerated in size ranging from 180 to 195 nm. The FTIR revealed no major changes in drug peaks. Encapsulation and loading efficiencies showed sufficient amount of drug being loaded into the NPs. The drug loaded NPs showed no change in size or zeta potential even after a period of 30 days. The cytotoxicity studies revealed significant reduction in toxicity after coating the surface treated with PLGA as evident from the microscopic analysis of cells. Hence the current study may be prioritized and further in vivo/in vitro studies may be carried out.

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“…3.10) software was used to fit the impedance data. Pradhan et al (Pradhan et al 2014 ) supplied the equivalent circuit for the impedance measurements explained in Fig. 5 .…”

Section: Methodsmentioning

confidence: 99%

Biosynthesis, characterization of PLGA coated folate-mediated multiple drug loaded copper oxide (CuO) nanoparticles and it’s cytotoxicity on nasopharyngeal cancer cell lines

Guo

Zhao

et al. 2020

AMB Expr

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“…3.10) to fit it. The equivalent circuit was acquired from a study by Pradhan et al 36 . RE and RI represent the solution resistance and charge transfer resistance, while CS and QM are the capacitance of water and interface impedance of cells.…”

Section: Impedance Measurementsmentioning

confidence: 99%

“…The ECIS device circuit of the biological cell was designed, as was adapted from a study by Pradhan et al 36 and is described in Fig. 8b.…”

Section: Impedance Measurementsmentioning

confidence: 99%

Theranostic combinatorial drug-loaded coated cubosomes for enhanced targeting and efficacy against cancer cells

et al. 2020

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Cubosomes, a product of nanobioengineering, are self-structured lipid nanoparticles that act like drug-loaded theranostic probes. Here, we describe a simple method for the preparation of combinatorial drug-loaded cubosomes with, proof-of-principle, therapeutic effect against cancer cells, along with diagnostic capabilities. Anticancer drugs cisplatin and paclitaxel were loaded in the cubosomes in combination. The cubosomes were coated with a layer of poly-Ɛ-lysine, which helped avoid the initial burst release of drug and allowed for a slow and sustained release for better efficacy. Cubosomes were imaged by transmission electron microscope, and their dispersion analyzed in vitro by differential scanning calorimetric and X-ray diffractogram studies. The microscopic images depicted spherical polyangular structures, which are easily distinguishable. The analyses revealed that the drug is uniformly dispersed all through the cubosomes. Further characterization was carried out by zeta-potential measurement, in vitro release, and entrapment efficiency studies. The in vitro studies established that the coating of cubosomes successfully reduced the burst release of drugs initially and confirmed a slow, sustained release over increased time. Comparative cytotoxicity of coated, uncoated, and blank cubosomes was evaluated, using human hepatoma HepG2 cell line, and the formulations were found to be entirely nontoxic, similar to the blank ones. The therapeutic efficiency of the cubosomes against HeLa cells was confirmed by the impedance measurement and fluorescent imaging. Furthermore, the reduction in impedance in cells treated with coated combinatorial cubosomes proved the impairment of HeLa cells, as confirmed by fluorescence microscopy.

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“…These sensors are now widely applied in detections of various scales, e.g. DNA [21][22][23] , proteins 24,25 , cells 26,27 and bacteria 28 . For impedance-based measurements, an electrode-electrolyte double layer will be formed at the electrode surface, and this double layer capacitance behaves as a parasitic component to reduce the sensitivity 29 .…”

Section: Introductionmentioning

confidence: 99%

Inkjet-printed Ag electrodes on paper for high sensitivity impedance measurements

Jiang

et al. 2016

RSC Adv.

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Electric cell–substrate impedance sensing technique to monitor cellular behaviours of cancer cells

Cited by 18 publications

References 33 publications

Biosynthesis, characterization of PLGA coated folate-mediated multiple drug loaded copper oxide (CuO) nanoparticles and it’s cytotoxicity on nasopharyngeal cancer cell lines

Biosynthesis, characterization of PLGA coated folate-mediated multiple drug loaded copper oxide (CuO) nanoparticles and it’s cytotoxicity on nasopharyngeal cancer cell lines

Theranostic combinatorial drug-loaded coated cubosomes for enhanced targeting and efficacy against cancer cells

Inkjet-printed Ag electrodes on paper for high sensitivity impedance measurements

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