Electrical signals regulate the release of insulin from electrodeposited chitosan composite hydrogel: An in vitro and in vivo study

Abstract

Electrical signal controlled drug release from polymeric drug delivery system provides an efficient way for accurate and demandable drug release. In this work, insulin was loaded on inorganic nanoplates (layered double hydroxides, LDHs) and coated on a copper wire by co‐electrodeposition with chitosan. The formed structure in chitosan composite hydrogel entrapped insulin efficiently, which were proved by various techniques. In addition, the drug loaded chitosan composite hydrogel demonstrated good biocompatibility as suggested by cell attachment. In vitro drug release experiment showed fast responsive pulsed release of insulin by biasing electrical signals. The in vivo experiment in diabetic rats revealed controllable insulin release in plasma and stable decrease of blood glucose can be achieved by using appropriate electrical signal. In addition, HE staining suggested negligible effect to the tissue by electrical signals. This work suggests that the electrical signal controlled insulin release from chitosan composited hydrogel may be a promising administration route for insulin.

Programmable Electrical Signals Induce Anisotropic Assembly of Multilayer Chitosan Hydrogels

“…The results can provide an application scenario for multilayer hydrogels in drug sustained release or molecular screening. 14,31 ■ CONCLUSIONS In this work, the growth kinetics and birefringence change of multilayer chitosan hydrogel were observed in real time by polarized light microscopy. The results show that a brief electrical signal interruption does not affect the anisotropic assembly process.…”

“…The multilayer hydrogel exhibits lower ion transport capacity because there are more boundaries than the monolayer hydrogel. The results can provide an application scenario for multilayer hydrogels in drug sustained release or molecular screening. , …”

“…Chitosan, as a cationic polysaccharide, exhibits excellent biocompatibility, biodegradability, and adaptability. 11−13 It has been reported that chitosan multilayer hydrogels are designed to meet various applications such as drug delivery, 14 tissue engineering, 15 and communication systems. 16 We have developed a simple and effective one-pot electrodeposition methodology to construct chitosan multilayer hydrogels.…”

“…Chitosan, as a cationic polysaccharide, exhibits excellent biocompatibility, biodegradability, and adaptability. − It has been reported that chitosan multilayer hydrogels are designed to meet various applications such as drug delivery, tissue engineering, and communication systems . We have developed a simple and effective one-pot electrodeposition methodology to construct chitosan multilayer hydrogels. , Short electrical signal interruptions can produce highly resolved boundaries within the bulk hydrogel, and oscillating electrical input signals are proven to have a strong effect on the structure of hydrogel.…”

Electroresponsive Thiol–Yne Click-Hydrogels for Insulin Smart Delivery: Tackling Sustained Release and Leakage Control

“…Stimuli-responsive systems represent an efficient strategy to tackle this issue and promote a better glycemic control. In hydrogel-based devices, the variety of stimuli that trigger insulin release include physiological temperature, , pH, − electrical current, − dynamic covalent bonding, or glucose itself, ,− among others …”