Electrical stimulation: Effective cue to direct osteogenic differentiation of mesenchymal stem cells?

Guillot-Ferriols, María; Lanceros‐Méndez, S.; Ribelles, J.L. Gómez; Ferrer, Gloria Gallego

doi:10.1016/j.bioadv.2022.212918

Cited by 30 publications

(21 citation statements)

References 164 publications

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“…The use of piezoelectric substrates to provide ES for cultured cells can be based on two mechanisms: i) the presence of an electroactive microenvironment due to the surface charge that may affect protein conformation and ii) enhanced cell adhesion. [ 68 ] These mechanisms trigger different mechanotransduction signaling pathways. Meanwhile, the ES of signaling pathways results in a cascade of processes [ 69 ] that are responsible for cellular behavior, such as extracellular–matrix production, cell growth, and tissue repair.…”

Section: Resultsmentioning

confidence: 99%

“…[ 73 ] Other possible signaling pathways could also make a contribution to the improved cell growth on the surface of piezoelectric PHB‐rGO scaffolds, e.g., bone morphogenetic protein receptors, the Notch signaling pathway, and the Wnt/ β ‐catenin signaling cascade activated by cell–cell connection with piezoelectric stimulation. [ 68 ] Hence, piezoactive scaffolds that mimic the extracellular matrix and the piezoresponse of natural tissues hold promise for the repair and regeneration of tissues, such as bone, cartilage, and nervous tissue. Moreover, piezoactive materials have great potential for control over stem cell differentiation.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, piezoactive materials have great potential for control over stem cell differentiation. [ 68 ]…”

Section: Resultsmentioning

confidence: 99%

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Cell Behavior Changes and Enzymatic Biodegradation of Hybrid Electrospun Poly(3‐hydroxybutyrate)‐Based Scaffolds with an Enhanced Piezoresponse after the Addition of Reduced Graphene Oxide

Chernozem

Pariy

Surmeneva

et al. 2023

Adv Healthcare Materials

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This is the first comprehensive study of the impact of biodegradation on the structure, surface potential, mechanical and piezoelectric properties of poly(3-hydroxybutyrate) (PHB) scaffolds supplemented with reduced graphene oxide (rGO) as well as cell behavior under static and dynamic mechanical conditions. There is no effect of the rGO addition up to 1.0 wt% on the rate of enzymatic biodegradation of PHB scaffolds for 30 d. The biodegradation of scaffolds leads to the depolymerization of the amorphous phase, resulting in an increase in the degree of crystallinity. Because of more regular dipole order in the crystalline phase, surface potential of all fibers increases after the biodegradation, with a maximum (361 ± 5 mV) after the addition of 1 wt% rGO into PHB as compared to pristine PHB fibers. By contrast, PHB-0.7rGO fibers manifest the strongest effective vertical (0.59 ± 0.03 pm V −1 ) and lateral (1.06 ± 0.02 pm V −1 ) piezoresponse owing to a greater presence of electroactive 𝜷-phase. In vitro assays involving primary human fibroblasts reveal equal biocompatibility and faster cell proliferation on PHB-0.7rGO scaffolds compared to pure PHB and nonpiezoelectric polycaprolactone scaffolds. Thus, the developed biodegradable PHB-rGO scaffolds with enhanced piezoresponse are promising for tissue-engineering applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Cell Behavior Changes and Enzymatic Biodegradation of Hybrid Electrospun Poly(3‐hydroxybutyrate)‐Based Scaffolds with an Enhanced Piezoresponse after the Addition of Reduced Graphene Oxide

Chernozem

Pariy

Surmeneva

et al. 2023

Adv Healthcare Materials

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“…Several factors may influence the effect of piezoelectric stimulation on MSCs differentiation towards the osteogenic lineage [ 49 ]. The selection of cell culture media, stimulation parameters and treatment times are non-trivial choices when designing stimulation experiments.…”

Section: Resultsmentioning

confidence: 99%

Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells

Guillot-Ferriols

García-Briega

Tolosa

et al. 2022

Gels

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Mesenchymal stem cells (MSCs) osteogenic commitment before injection enhances bone regeneration therapy results. Piezoelectric stimulation may be an effective cue to promote MSCs pre-differentiation, and poly(vinylidene) fluoride (PVDF) cell culture supports, when combined with CoFe2O4 (CFO), offer a wireless in vitro stimulation strategy. Under an external magnetic field, CFO shift and magnetostriction deform the polymer matrix varying the polymer surface charge due to the piezoelectric effect. To test the effect of piezoelectric stimulation on MSCs, our approach is based on a gelatin hydrogel with embedded MSCs and PVDF-CFO electroactive microspheres. Microspheres were produced by electrospray technique, favouring CFO incorporation, crystallisation in β-phase (85 %) and a crystallinity degree of around 55 %. The absence of cytotoxicity of the 3D construct was confirmed 24 h after cell encapsulation. Cells were viable, evenly distributed in the hydrogel matrix and surrounded by microspheres, allowing local stimulation. Hydrogels were stimulated using a magnetic bioreactor, and no significant changes were observed in MSCs proliferation in the short or long term. Nevertheless, piezoelectric stimulation upregulated RUNX2 expression after 7 days, indicating the activation of the osteogenic differentiation pathway. These results open the door for optimising a stimulation protocol allowing the application of the magnetically activated 3D electroactive cell culture support for MSCs pre-differentiation before transplantation.

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“…It was understood that several key criteria should also be considered when fabricating the synthetic tissue models with extrinsic mechanical stimuli [14,15]. Thus, so far, it was understood that extrinsic mechanical stimuli would interact with the specific cell receptors known as mechanotransduction receptors and pathways.…”

Section: Introductionmentioning

confidence: 99%

The Synergistic Effect of Cyclic Tensile Force and Periodontal Ligament Cell-Laden Calcium Silicate/Gelatin Methacrylate Auxetic Hydrogel Scaffolds for Bone Regeneration

Lee

Lin

et al. 2022

Cells

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The development of 3D printing technologies has allowed us to fabricate complex novel scaffolds for bone regeneration. In this study, we reported the incorporation of different concentrations of calcium silicate (CS) powder into fish gelatin methacrylate (FGelMa) for the fabrication of CS/FGelMa auxetic bio-scaffolds using 3D printing technology. Our results showed that CS could be successfully incorporated into FGelMa without influencing the original structural components of FGelMa. Furthermore, it conveyed that CS modifications both the mechanical properties and degradation rates of the scaffolds were improved in accordance with the concentrations of CS upon modifications of CS. In addition, the presence of CS enhanced the adhesion and proliferation of human periodontal ligament cells (hPDLs) cultured in the scaffold. Further osteogenic evaluation also confirmed that CS was able to enhance the osteogenic capabilities via activation of downstream intracellular factors such as pFAK/FAK and pERK/ERK. More interestingly, it was noted that the application of extrinsic biomechanical stimulation to the auxetic scaffolds further enhanced the proliferation and differentiation of hPDLs cells and secretion of osteogenic-related markers when compared to CS/FGelMa hydrogels without tensile stimulation. This prompted us to explore the related mechanism behind this interesting phenomenon. Subsequent studies showed that biomechanical stimulation works via YAP, which is a biomechanical cue. Taken together, our results showed that novel auxetic scaffolds could be fabricated by combining different aspects of science and technology, in order to improve the future chances of clinical applications for bone regeneration.

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Electrical stimulation: Effective cue to direct osteogenic differentiation of mesenchymal stem cells?

Cited by 30 publications

References 164 publications

Cell Behavior Changes and Enzymatic Biodegradation of Hybrid Electrospun Poly(3‐hydroxybutyrate)‐Based Scaffolds with an Enhanced Piezoresponse after the Addition of Reduced Graphene Oxide

Cell Behavior Changes and Enzymatic Biodegradation of Hybrid Electrospun Poly(3‐hydroxybutyrate)‐Based Scaffolds with an Enhanced Piezoresponse after the Addition of Reduced Graphene Oxide

Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells

The Synergistic Effect of Cyclic Tensile Force and Periodontal Ligament Cell-Laden Calcium Silicate/Gelatin Methacrylate Auxetic Hydrogel Scaffolds for Bone Regeneration

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