Electrical stimulation promotes the proliferation of human keratinocytes, increases the production of keratin 5 and 14, and increases the phosphorylation of ERK1/2 and p38 MAP kinases

Rouabhia, Mahmoud; Park, Hyun Jin; Abedin-Do, Atieh; Douville, Yvan; Méthot, Mireille; Zhang, Ze

doi:10.1002/term.3040

Cited by 24 publications

(23 citation statements)

References 32 publications

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“…When the two epithelial tongues fuse at the middle of the wound, contact inhibition stops the migratory process of keratinocytes, and they undergo programmed terminal differentiation to result in epidermal stratification. ES has been shown to enhance both migration and proliferation of keratinocyte in the process of epithelization [ 30 , 31 ]. To address the concern whether NG-ES leads to excessive-epithelization, we quantified the thickness of the newly regenerated epidermis at the wound site between the NG-ES and the control groups (Additional file 2 : Figure S5).…”

Section: Resultsmentioning

confidence: 99%

Accelerated complete human skin architecture restoration after wounding by nanogenerator-driven electrostimulation

Liu

Long

et al. 2021

J Nanobiotechnol

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Background Electrostimulation (ES) therapy for wound healing is limited in clinical use due to barriers such as cumbersome equipment and intermittent delivery of therapy. Methods We adapted a human skin xenograft model that can be used to directly examine the nanogenerator-driven ES (NG-ES) effects on human skin in vivo—an essential translational step toward clinical application of the NG-ES technique for wound healing. Results We show that NG-ES leads to rapid wound closure with complete restoration of normal skin architecture within 7 days compared to more than 30 days in the literature. NG-ES accelerates the inflammatory phase of wound healing with more rapid resolution of neutrophils and macrophages and enhances wound bed perfusion with more robust neovascularization. Conclusion Our results support the translational evaluation and optimization of the NG-ES technology to deliver convenient, efficient wound healing therapy for use in human wounds. Graphic abstract

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Section: Resultsmentioning

confidence: 99%

Accelerated complete human skin architecture restoration after wounding by nanogenerator-driven electrostimulation

Liu

Long

et al. 2021

J Nanobiotechnol

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“…Normal and diabetic human skin fibroblasts were isolated from skin tissues collected from non‐diabetic and diabetic patients (61–80 years old) following leg amputation surgery, as previously reported (Rouabhia et al., 2020). Cells were used in passages 4–6 were seeded (10 5 cells/cm 2 ) onto the PPy/HE/PLLA membranes in the electrical cell culture device and cultured for 24 h. Cells were exposed to ES at 20 and 40 mV/mm 2 intensities for six or 24 h, and cultured for an additional 48 h post‐exposure to ES (for more detail, see Supplementay Information).…”

Section: Methodsmentioning

confidence: 99%

“…DHSF were seeded onto PPy/HE/PLLA membranes and cultured for 3 days to obtain confluent fibroblast monolayers before being exposed or not to ES at 20 or 40 mV/mm 2 for 6 or 24 h. Immediately after each ES regime, two vertical scratches were made on the cell monolayers at the surface of each PPy/HE/PLLA membrane, as we previously reported (Rouabhia et al., 2020). Cell migration was subsequently measured at different times: 0, 12, 24, 36, and 48 h after scratch, using Hoechst staining assay, observed under an epifluorescence microscope, and analyzed with NIH‐ImageJ 1.47v software.…”

Section: Methodsmentioning

confidence: 99%

“…Following the exposure to Es, cell proliferation was measured using a thiazolyl tetrazolium salt (MTS) colorimetric assay (Cui et al, 2021). This study section as we previously reported (Rouabhia et al, 2020). Cell migration was subsequently measured at different times: 0, 12, 24, 36, and 48 h after scratch, using Hoechst staining assay, observed under an epifluorescence microscope, and analyzed with NIH-ImageJ 1.47v software.…”

Section: Primary Diabetic Human Skin Fibroblast Culturementioning

confidence: 99%

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Electrical stimulation promotes the wound‐healing properties of diabetic human skin fibroblasts

Abedin-Do

Zhang

Douville

et al. 2022

J Tissue Eng Regen Med

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This study evaluated the effect of low (20 and 40 mV/mm) intensities of electrical stimulation on the proliferation and migration of skin fibroblasts from diabetic donors.We also examined the effect of electrical stimulation on modulating the capacity of fibroblasts to contract collagen gel, express alpha-smooth muscle actin, and secrete proteolytic enzymes involved in regulating extracellular matrix synthesis and degradation. Our study shows that 20 and 40 mV/mm of stimulation increased the growth of fibroblasts extracted from diabetic patients but not from non-diabetic donors. Electrical stimulation increased the migration of diabetic fibroblasts, their capacity to contract collagen gel, and the expression of alpha-smooth muscle actin and promoted different proteolytic enzymes involved in accelerating wound healing.Overall results confirm the effectiveness of electrical stimulation in modulating the wound healing activities of fibroblasts extracted from diabetic skin donors. This study, therefore, suggests the possible use of electrical stimulation to promote diabetic foot ulcer healing by stimulating the wound healing properties of skin fibroblasts.

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“…A study on E. coli found that DC caused two-way leakage in the membrane that is large enough to make way for protein leaking, thus killing the bacteria [44]. Another study using AC (5 and 20 mA) inhibited growth of Pseudomonas aeruginosa, but no effect was seen on Escherichia coli and Staphylococcus aureus [45].…”

Section: Inflammatory Phasementioning

confidence: 99%

Wound Healing with Electrical Stimulation Technologies: A Review

2021

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Electrical stimulation (ES) is an attractive field among clinicians in the topic of wound healing, which is common yet complicated and requires multidisciplinary approaches. The conventional dressing and skin graft showed no promise on complete wound closure. These urge the need for the exploration of electrical stimulation to supplement current wound care management. This review aims to provide an overview of electrical stimulation in wound healing. The mechanism of galvanotaxis related to wound repair will be reviewed at the cellular and molecular levels. Meanwhile, different modalities of externally applied electricity mimicking a physiologic electric field will be discussed and compared in vitro, in vivo, and clinically. With the emerging of tissue engineering and regenerative medicine, the integration of electroconductive biomaterials into modern miniaturised dressing is of interest and has become possible with the advancing understanding of smart biomaterials.

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Electrical stimulation promotes the proliferation of human keratinocytes, increases the production of keratin 5 and 14, and increases the phosphorylation of ERK1/2 and p38 MAP kinases

Cited by 24 publications

References 32 publications

Accelerated complete human skin architecture restoration after wounding by nanogenerator-driven electrostimulation

Accelerated complete human skin architecture restoration after wounding by nanogenerator-driven electrostimulation

Electrical stimulation promotes the wound‐healing properties of diabetic human skin fibroblasts

Wound Healing with Electrical Stimulation Technologies: A Review

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