Electrical stunning in pacu (Piaractus mesopotamicus) using direct current waveform

Rucinque, Daniel Santiago; Watanabe, André Luiz; Molento, Carla Forte Maiolino

doi:10.1016/j.aquaculture.2018.07.035

Cited by 15 publications

(13 citation statements)

References 35 publications

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“…Any lingering effects of the sedation agent may contribute to alterations in natural behaviour (Losey & Hugie, 1994;Mettam et al, 2011;Prystay et al, 2017) or lead to postrelease predation (reviewed in Raby et al, 2014). Lingering effects would be suboptimal for studies in which post-release behaviour is monitored (i.e., telemetry studies; see Brownscombe et al, 2019) g., Chatakondi & Kelly, 2019;Nguyen et al, 2018;Rucinque et al, 2018;Trushenski et al, 2017). Nonetheless, it is possible, and often preferable, to sample fish from the caudal vasculature without the use of any anaesthesia (as described later).…”

Section: Many Of the Anaesthesia Methods Discussed May Extend Captivitymentioning

confidence: 99%

“…Low‐voltage electricity has been proposed as an anaesthetic to aid in fish restraint because of relatively short recovery times after exposure ( i.e ., seconds; Vandergoot et al ., 2011; Trushenski & Bowker, 2012; Ward et al ., 2017; Abrams et al ., 2018; Reid et al ., 2019). Indeed, because of its relatively low and transient impacts, electricity is widely used as an anaesthetic in aquaculture ( e.g ., Chatakondi & Kelly, 2019; Nguyen et al ., 2018; Rucinque et al ., 2018; Trushenski et al ., 2017). Nonetheless, it is possible, and often preferable, to sample fish from the caudal vasculature without the use of any anaesthesia (as described later).…”

Section: The Use Of Anaesthesia In Caudal Puncturementioning

confidence: 99%

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Best practices for non‐lethal blood sampling of fish via the caudal vasculature

Lawrence

Raby

Teffer

et al. 2020

Journal of Fish Biology

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Blood sampling through the caudal vasculature is a widely used technique in fish biology for investigating organismal health and physiology. In live fishes, it can provide a quick, easy and relatively non-invasive method for obtaining a blood sample (cf. cannulation and cardiac puncture). Here, a general set of recommendations are provided for optimizing the blood sampling protocol that reflects best practices in animal welfare and sample integrity. This includes selecting appropriate use of anaesthetics for blood sampling as well as restraint techniques for situations where sedation is not used. In addition, ideal sampling environments where the fish can freely ventilate and strategies for minimizing handling time are discussed. This study summarizes the techniques used for extracting blood from the caudal vasculature in live fishes, highlighting the phlebotomy itself, the timing of sampling events and acceptable blood sample volumes. This study further discuss considerations for selecting appropriate physiological metrics when sampling in the caudal region and the potential benefits that this technique provides with respect to long-term biological assessments. Although general guidelines for blood sampling are provided here, it should be recognized that contextual considerations (e.g., taxonomic diversity, legal matters, environmental constraints) may influence the approach to blood sampling. Overall, it can be concluded that when done properly, blood sampling live fishes through the caudal vasculature is quick, efficient and minimally invasive, thus promoting conditions where live release of focal animals is possible.

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Section: Many Of the Anaesthesia Methods Discussed May Extend Captivitymentioning

confidence: 99%

Section: The Use Of Anaesthesia In Caudal Puncturementioning

confidence: 99%

Best practices for non‐lethal blood sampling of fish via the caudal vasculature

Lawrence

Raby

Teffer

et al. 2020

Journal of Fish Biology

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“…The effect of the electrical treatment will depend on the strength and duration of the current as well as the frequency of the electrical waveform (Lines & Kestin, 2004). When applied correctly, percussive or electrical stunning methods appear to achieve humane slaughter under appropriate conditions (Rucinque, Watanabe, & Molento, 2018).…”

Section: Introductionmentioning

confidence: 99%

Stress assessment, quality indicators and shelf life of three aquaculture important marine fish, in relation to harvest practices, water temperature and slaughter method

Papaharisis¹,

Tsironi

Dimitroglou³

et al. 2019

Aquac Res

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The objective of this study was to evaluate the effect of harvest practices and slaughter method on stress, quality and shelf life of whole fish (gilthead sea bream, European sea bass and red sea bream) towards the development of a humane slaughter practice. The use of hook and line resulted in significantly lower plasma cortisol and glucose in European sea bass and gilthead sea bream. Water temperature at harvest affected significantly the concentrations of stress indicators (plasma cortisol and glucose), mainly in European sea bass and gilthead sea bream. No significant differences were observed between the harvesting methods, regarding fish appearance, microbial spoilage and sensory scoring and subsequently shelf life. However, significantly lower hardness was recorded in fish harvested using the electric stunner, compared to the fish killed with slurry ice of the same harvesting period. Shelf life ranged between 13-18 days for gilthead seabream, 10-16 days for European sea bass and 10-14 days for red sea bream, depending on harvesting period. K E Y W O R D Sfish welfare, harvest, quality, shelf life, stress, water temperature | 2609 PAPAHARISIS et Al.

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“…Previous studies evidenced vertebral fractures and consequent blood spots in fillets of fishes exposed to electrical stunning, which negatively affect fillet quality. 19,51 Conversely, other authors did not evidence spinal damage or haematomas as post-filleting quality imperfections, 11,52,53 indicating that this method was properly applied in terms of voltage and exposure time. In the present study, 55% of ES fillets showed haematomas at 0 h, in line with what was observed in Chinook salmon (24-71%) 51 and Atlantic herring (60%), 19 hence highlighting the need to set specific electrical stunning parameters for S. carpio.…”

Section: Fillet Shape and Colour Changesmentioning

confidence: 90%

Effect of different stunning methods on rigor mortis, shape, energetic status and physical characteristics of Salmo carpio fillets

et al. 2022

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BACKGROUND Consumer interest in safeguarding animal welfare and increased demand for fresh aquatic products support the need to understand the effects of stunning methods used in aquaculture on the biochemical process affecting fish fillet quality. The present paper aimed at comparing electrical stunning (ES) and cold shock (ICE) in Salmo carpio, an Italian endemic under‐investigated species. Rigor mortis evolution, fillet adenosine 5′‐triphosphate (ATP), shape, colour, pH and water holding capacity were assessed by integrating chemical and image analyses. RESULTS Seventy‐two fish (24 fish per treatment) were stunned by ES, ICE or anaesthesia (AN, used as control), then percussively slaughtered. ES and ICE hastened rigor mortis onset and resolution (21 and 28 h post mortem) compared to AN. This was confirmed by the faster ATP degradation in ES and ICE. Fillet shape features varied during rigor mortis, according to the stunning method, with the perimeter showing irreversible variation in ES and ICE groups. Initial circularity was recovered only in AN, while ICE and ES fillets showed significantly different values, between 0 and 192 h. CONCLUSION ES is a promising stunning technique for S. carpio, but parameters should be optimized, because of the adverse effect on muscle activity which caused a fast pH drop, and the presence of blood spots in the fillets. Further studies are needed to understand whether fillet shape changes can interfere with filleting or fillet processing and consumer appreciation. © 2022 Society of Chemical Industry.

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Electrical stunning in pacu (Piaractus mesopotamicus) using direct current waveform

Cited by 15 publications

References 35 publications

Best practices for non‐lethal blood sampling of fish via the caudal vasculature

Best practices for non‐lethal blood sampling of fish via the caudal vasculature

Stress assessment, quality indicators and shelf life of three aquaculture important marine fish, in relation to harvest practices, water temperature and slaughter method

Effect of different stunning methods on rigor mortis, shape, energetic status and physical characteristics of Salmo carpio fillets

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