Electrically controlled proliferation of human carcinoma cells cultured on the surface of an electrode

Kojima, Junichiro; Shinohara, Hiroaki; Ikariyama, Yoshihito; Aizawa, Masuo; Nagaike, Kazuhiro; Morioka, Satoshi

doi:10.1016/0168-1656(91)90241-m

Cited by 36 publications

(14 citation statements)

References 16 publications

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“…15 Previous study has found that electrical stimulation cause cells to temporarily halt proliferation by decreasing their membrane fluidity. 17 Therefore, one possible result of these changes in membrane fluidity is linked to alterations in the physiological processes of the cell membrane, such as carrier-mediated transport, activity of membranebound enzyme, and membrane fluorescence anisotropy. 16,17 This linkage was also revealed after cells FIGURE 4.…”

Section: Discussionmentioning

confidence: 99%

“…17 Therefore, one possible result of these changes in membrane fluidity is linked to alterations in the physiological processes of the cell membrane, such as carrier-mediated transport, activity of membranebound enzyme, and membrane fluorescence anisotropy. 16,17 This linkage was also revealed after cells FIGURE 4. After 48 h of SMF exposure: (a) the alkaline phosphatase activity of the SMF-exposed cells was significantly greater than that of the controls (n = 4; *p < 0.05); the greatest difference between the untreated control (b) and SMF-exposed cells (c) was in the extracellular areas from TEM.…”

Section: Discussionmentioning

confidence: 99%

“…3a-3c). During the 3-day tests, there was a tendency towards increased cell percentage in the G 0 /G 1 phase (approximately 55, 55, 60, and 75% at 0, 24, 48, and 72 h, respectively), with fewer cells in the S and G 2 /M phases (25,26,17, and 11% at 0, 24, 48, and 72 h, respectively). However, in each cell-cycle phase, significant differences were not demonstrated comparing the exposed and unexposed cells at each observation time.…”

Section: Statistically Significant Differences In Cell Numbersmentioning

confidence: 93%

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Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Chiu

Lee

et al. 2007

Ann Biomed Eng

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The aim of this study was to test the differentiative effects of osteoblasts after treatment with a static magnetic field (SMF). MG63 osteoblast-like cells were exposed to a 0.4-T SMF. The differentiation markers were assessed by observing the changes in alkaline phosphatase activity and electron microscopy images. Membrane fluidity was used to evaluate alterations in the biophysical properties of the cellular membranes after the SMF simulation. Our results show that SMF exposure increases alkaline phosphatase activity and extracellular matrix release in MG63 cells. On the other hand, MG63 cells exposed to a 0.4-T SMF exhibited a significant increase in fluorescence anisotropy at 6 h, with a significant reduction in the proliferation effects of growth factors noted at 24 h. Based on these findings, the authors suggest that one of the possible mechanisms that SMF affects osteoblastic maturation is by increasing the membrane rigidity and reducing the proliferation-promoting effects of growth factors at the membrane domain.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Statistically Significant Differences In Cell Numbersmentioning

confidence: 93%

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Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Chiu

Lee

et al. 2007

Ann Biomed Eng

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“…A previous study reported that a lowering of cell membrane fluidity was caused not only by electrical stimulation (Kojima et al 1991), but also by certain medicines and by changes in temperature. Another study demonstrated that use of the local anesthetic xylocaine affected the fluidity of the cell plasma membrane, in turn affecting HIV-1 infectivity (Harada et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, cell membrane damage (Tominaga et al 2007), the regulation of cell proliferation (Kojima et al 1991; Yaoita et al 1990), gene expression of nerve growth factor (Koyama et al 1996; Koyama et al 1997) and neural and osteogenic differentiation (Kimura et al 1998; Mie et al 2003), have been reported in response to low potential loading. Furthermore, the combined effect of low potential stimulation and cisplatin administration caused cell death in HeLa cells (Manabe et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Sensitivity to electrical stimulation of human immunodeficiency virus type 1 and MAGIC-5 cells

2011

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To determine the sensitivities to low electrical potential of human immunodeficiency virus type 1 (HIV-1) and its target cells, HIV-1 and MAGIC-5 cells were directly stimulated with a constant direct current potential of 1.0 V (vs. Ag/AgCl). HIV-1 was incubated for 3 h at 37°C on a poly-L-lysine-coated indium-tin oxide electrode, and then stimulated by an electrical potential. MAGIC-5 cells were seeded onto the electrically stimulated HIV-1 and cultured for 3 days at 37°C. HIV-1-infected cells were measured by multinuclear activation via a galactosidase indicator assay. MAGIC-5 cells were also stimulated by an electrical potential of 1.0 V; cell damage, proliferation and apoptosis were evaluated by trypan blue staining, cell counting and in situ apoptosis detection, respectively. HIV-1 was found to be damaged to a greater extent by electrical stimulation than the cells. In particular, after application of a 1.0-V potential for 3 min, HIV-1LAI and HIV-1KMT infection were inhibited by about 90%, but changes in cell damage, proliferation and apoptosis were virtually undetectable. These results suggested that HIV-1 is significantly more susceptible to low electrical potential than cells. This finding could form the basis of a novel therapeutic strategy against HIV-1 infection.

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Manipulating and Monitoring Biomolecular Interactions with Conducting Electroactive Polymers

Wallace¹,

Kane-Maguire²

2002

Adv. Mater.

122

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Electrically controlled proliferation of human carcinoma cells cultured on the surface of an electrode

Cited by 36 publications

References 16 publications

Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Sensitivity to electrical stimulation of human immunodeficiency virus type 1 and MAGIC-5 cells

Manipulating and Monitoring Biomolecular Interactions with Conducting Electroactive Polymers

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