Electrically-responsive antimicrobial coatings based on a tetracycline-loaded poly(3,4-ethylenedioxythiophene) matrix

Czerwińska-Główka, Dominika; Przystaś, Wioletta; Zabłocka-Godlewska, Ewa; Student, Sebastian; Cwalina, B.; Łapkowski, Mieczysław; Krukiewicz, Katarzyna

doi:10.1016/j.msec.2021.112017

Cited by 17 publications

(12 citation statements)

References 76 publications

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“…Conducting polymers can also be employed as antibiotic carriers that enable electrically-triggered controlled drug release and electrically-driven antibacterial activity. In a recent study [ 137 ], a PEDOT matrix was used to immobilize a powerful first-line antibiotic (tetracycline; Tc). Although a portion of the immobilized Tc was released spontaneously, the electrical trigger (a chronoamperometric potential jump from –0.6 V to –0.5 V, applied for 2 s and 600 s, respectively) made it possible to control the overall rate of drug release.…”

Section: Control Strategies For the Formation Of Clinically Important...mentioning

confidence: 99%

Recent Advances in the Control of Clinically Important Biofilms

Krukiewicz

Kazek-Kęsik

Brzychczy-Włoch

et al. 2022

IJMS

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Biofilms are complex structures formed by bacteria, fungi, or even viruses on biotic and abiotic surfaces, and they can be found in almost any part of the human body. The prevalence of biofilm-associated diseases has increased in recent years, mainly because of the frequent use of indwelling medical devices that create opportunities for clinically important bacteria and fungi to form biofilms either on the device or on the neighboring tissues. As a result of their resistance to antibiotics and host immunity factors, biofilms have been associated with the development or persistence of several clinically important diseases. The inability to completely eradicate biofilms drastically increases the burden of disease on both the patient and the healthcare system. Therefore, it is crucial to develop innovative ways to tackle the growth and development of biofilms. This review focuses on dental- and implant-associated biofilm infections, their prevalence in humans, and potential therapeutic intervention strategies, including the recent advances in pharmacology and biomedical engineering. It lists current strategies used to control the formation of clinically important biofilms, including novel antibiotics and their carriers, antiseptics and disinfectants, small molecule anti-biofilm agents, surface treatment strategies, and nanostructure functionalization, as well as multifunctional coatings particularly suitable for providing antibacterial effects to the surface of implants, to treat either dental- or implant-related bacterial infections.

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Section: Control Strategies For the Formation Of Clinically Important...mentioning

confidence: 99%

Recent Advances in the Control of Clinically Important Biofilms

Krukiewicz

Kazek-Kęsik

Brzychczy-Włoch

et al. 2022

IJMS

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“…In our research, we used SEM imaging to visualise the morphology of two types of biological samples representing prokaryotic and eukaryotic cells: a model Gram-negative bacterial strain Escherichia coli (DSM 30083, U5/41), and a cultured cell model of central nervous system neurons, namely rat neuroblastoma cell line B-35 (ATCC ® CRL-2754™). The details of culturing both types of cells can be found in our previous reports [ 43 , 44 , 45 ]. According to the optimised sample preparation protocol [ 43 , 44 , 45 ], cells were fixed using 3% glutaraldehyde for 24 h, then washed three times with sterile distilled water.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The details of culturing both types of cells can be found in our previous reports [ 43 , 44 , 45 ]. According to the optimised sample preparation protocol [ 43 , 44 , 45 ], cells were fixed using 3% glutaraldehyde for 24 h, then washed three times with sterile distilled water. Subsequently, samples were dehydrated by immersing them for 10 min in the solutions of ethanol with increasing concentrations (30%, 50%, 70%, 80%, 90%, 95%, 99.8%), then dried for 24 h at 50 °C.…”

Section: Sample Preparationmentioning

confidence: 99%

“…SEM is widely used in microbiological analysis to assess the morphology of bacterial cells, their adhesion to the surface, as well as their tendency to form bacterial biofilms [ 43 , 44 , 45 ]. Moreover, SEM allows to estimate the number and distribution of microorganisms on the investigated surface [ 43 ].…”

Section: Morphometric Analysis Of Model Prokaryotic Cells: Escherichia Colimentioning

confidence: 99%

“…Moreover, SEM allows to estimate the number and distribution of microorganisms on the investigated surface [ 43 ]. The versatility of results that can be collected by SEM analysis makes this type of microscopy favourable for the evaluation of antimicrobial character of medical surfaces [ 43 ], effectiveness of new antibiotic agents [ 47 ] or bioactive materials [ 44 , 45 ]. What is more, a high resolving power of SEM allows obtaining reliable information on the state of microorganisms in their natural environment [ 47 , 48 , 49 ].…”

Section: Morphometric Analysis Of Model Prokaryotic Cells: Escherichia Colimentioning

confidence: 99%

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Guidelines for a Morphometric Analysis of Prokaryotic and Eukaryotic Cells by Scanning Electron Microscopy

Czerwińska-Główka

Krukiewicz

2021

Cells

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The invention of a scanning electron microscopy (SEM) pushed the imaging methods and allowed for the observation of cell details with a high resolution. Currently, SEM appears as an extremely useful tool to analyse the morphology of biological samples. The aim of this paper is to provide a set of guidelines for using SEM to analyse morphology of prokaryotic and eukaryotic cells, taking as model cases Escherichia coli bacteria and B-35 rat neuroblastoma cells. Herein, we discuss the necessity of a careful sample preparation and provide an optimised protocol that allows to observe the details of cell ultrastructure (≥ 50 nm) with a minimum processing effort. Highlighting the versatility of morphometric descriptors, we present the most informative parameters and couple them with molecular processes. In this way, we indicate the wide range of information that can be collected through SEM imaging of biological materials that makes SEM a convenient screening method to detect cell pathology.

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Electroactive Polymers for On‐Demand Drug Release

Alkahtani,

Elbadawi,

Chapman

et al. 2023

Adv Healthcare Materials

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Conductive materials have played a significant role in advancing society into the digital era. Such materials are able to harness the power of electricity and are used to control many aspects of our daily lives. Conductive polymers (CPs) are an emerging group of polymers that possess metal‐like conductivity yet retain desirable polymeric features, such as processability, mechanical properties and biodegradability. Upon receiving an electrical stimulus, CPs can be tailored to achieve a number of responses, such as harvesting energy, biosensing and stimulating tissue growth. The recent FDA approval of a CP‐based material for a medical device has invigorated their research in healthcare, including as biosensors, tissue engineering grafts and implants. In drug delivery, CPs can act as electrical switches, drug release is achieved at a flick of a switch, thereby providing unprecedented control over drug release. In this review, recent developments in CP as electro‐active polymers for voltage‐stimuli responsive drug delivery systems are evaluated. The review demonstrates the distinct drug release profiles achieved by electro‐active formulations, and both the precision and ease of stimuli response. This level of dynamism promises to yield “smart medicines” and warrants further research. The review concludes by providing an outlook to electro‐active formulations in drug delivery and highlighting their integral roles in healthcare IoT.This article is protected by copyright. All rights reserved

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Electrically-responsive antimicrobial coatings based on a tetracycline-loaded poly(3,4-ethylenedioxythiophene) matrix

Cited by 17 publications

References 76 publications

Recent Advances in the Control of Clinically Important Biofilms

Recent Advances in the Control of Clinically Important Biofilms

Guidelines for a Morphometric Analysis of Prokaryotic and Eukaryotic Cells by Scanning Electron Microscopy

Electroactive Polymers for On‐Demand Drug Release

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