Electrically stimulated rupture of cell membranes with a conducting polymer-coated electrode

Shinohara, Hiroaki; Kojima, Junichiro; Yaoita, Masashi; Aizawa, Mamoru

doi:10.1016/0302-4598(89)85027-5

Cited by 18 publications

(2 citation statements)

References 11 publications

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“…A pioneering work showed that human erythrocytes and leucocytes precipitate on metallic surfaces at a given value of the applied potential [29]. A shift of the potential applied to polypyrrole-coated electrodes can drive membrane rupture and cell lysis of erythrocytes [30]. It has even been claimed that the potential is the only determining factor in the interaction between carbon surface and blood [31].…”

Section: Introductionmentioning

confidence: 99%

The electrochemical potential is a key parameter for cell adhesion and proliferation on carbon surface

Guette-Marquet

Basseguy

Roques

et al. 2022

Bioelectrochemistry

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Section: Introductionmentioning

confidence: 99%

The electrochemical potential is a key parameter for cell adhesion and proliferation on carbon surface

Guette-Marquet

Basseguy

Roques

et al. 2022

Bioelectrochemistry

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“…Much work was carried out in the Late Eighties by Aizawa's group at the Tokyo Institute of Technology. They found that electrochemical oxidation of PPy caused a large local pH change along with incorporation of anions from solution (Shinohara et al 1989a) and explored the rupture of erythrocyte cell membranes by an applied potential (Shinohara et al 1989b). They attributed the pH change to local OH K transfer between the electrolyte and the PPy film.…”

Section: Stimulated Polypyrrolementioning

confidence: 99%

Polypyrrole-based conducting polymers and interactions with biological tissues

Ateh

Navsaria

Vadgama

2006

J. R. Soc. Interface.

461

260

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Polypyrrole (PPy) is a conjugated polymer that displays particular electronic properties including conductivity. In biomedical applications, it is usually electrochemically generated with the incorporation of any anionic species including also negatively charged biological macromolecules such as proteins and polysaccharides to give composite materials. In biomedical research, it has mainly been assessed for its role as a reporting interface in biosensors. However, there is an increasing literature on the application of PPy as a potentially electrically addressable tissue/cell support substrate. Here, we review studies that have considered such PPy based conducting polymers in direct contact with biological tissues and conclude that due to its versatile functional properties, it could contribute to a new generation of biomaterials.

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Incorporation of Erythrocytes into Polypyrrole to Form the Basis of a Biosensor to Screen for Rhesus (D) Blood Groups and Rhesus (D) Antibodies

1999

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Antibodies to Rhesus (Rh) antigens are important indicators in screening for haemolytic disease of the new‐born (HDN) and autoimmune haemolytic anaemia (AIHA). Identification of the Rh antibodies formed by immune stimulation is also essential in order to maximize the in vivo survival time of transfused erythrocytes. Currently this is performed by agglutination based assays that are time consuming. A prototype of an immuno‐biosensor for detecting antibodies recognizing the Rhesus blood group antigen, Rh (D), was constructed. Human erythrocytes were incorporated into a conducting polypyrrole, polyelectrolyte matrix. The process was followed by using oximetry and light microscopy to demonstrate the integrity of the erythrocytes in the polymerization solution and in the polymer matrix; cyclic voltammetry and resistometry for electrochemical characterization of the polymer and then agglutination, ELISA techniques and cyclic resistometry for analysis of the immuno response from antigen/antibody binding. Antigen/antibody binding could be detected qualitatively by using resistometry while cycling the polymer between +0.35 V and –0.7 V (vs. Ag/AgCl). A characteristic cyclic change in resistance (a resistogram) was recorded. After addition of Anti‐Rh (D) antibody (250 µg/mL), the change in resistance during the resistogram decreased by 1.1 Ω (p<0.0008) in polymers containing Rh (D) positive erythrocytes, whereas polymers without erythrocytes showed no significant change.

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Electrically stimulated rupture of cell membranes with a conducting polymer-coated electrode

Cited by 18 publications

References 11 publications

The electrochemical potential is a key parameter for cell adhesion and proliferation on carbon surface

The electrochemical potential is a key parameter for cell adhesion and proliferation on carbon surface

Polypyrrole-based conducting polymers and interactions with biological tissues

Incorporation of Erythrocytes into Polypyrrole to Form the Basis of a Biosensor to Screen for Rhesus (D) Blood Groups and Rhesus (D) Antibodies

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