Electro-acupuncture attenuates the mice premature ovarian failure via mediating PI3K/AKT/mTOR pathway

Zhang, Han; Qin, Fenfen; Liu, Anlong; Sun, Qinmei; Wang, Qisheng; Xie, Shuwu; Shen, Lu; Zhang, Dong; Lu, Zhigang

doi:10.1016/j.lfs.2018.11.059

Cited by 47 publications

(35 citation statements)

References 24 publications

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“…We found that CD4 + CD25 + Tregs partially rescued the effects of pZP3 via multiple mechanisms, including inhibition of the apoptosis conditions in the ovaries, regulation of the secretion of hormones and follicle development, as described in other reports (Li & Liu, 2018;Ling et al, 2019;Zhang et al, 2019). Data also showed that CD4 + CD25 + Tregs significantly decreased Akt and FOXO3a expression in ovarian tissues.…”

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confidence: 83%

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Adoptive transfers of CD4⁺CD25⁺ Tregs partially alleviate mouse premature ovarian insufficiency

Liú

Chi-bing

et al. 2020

Molecular Reproduction Devel

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This study was designed to investigate the protective effect of CD4+CD25+ regulatory T cells (Tregs) against zona pellucida glycoprotein 3 peptide (pZP3) immunization‐induced premature ovarian insufficiency (POI) in mice. A mouse POI model was induced by two subcutaneous injections of pZP3 (50 nmol/L). Mice in the pZP3‐Treg group were intraperitoneally injected with 5 × 105 CD4+CD25+ Tregs after the POI model was established. Sex hormone levels, follicle numbers, apoptotic events, and the Akt/FOXO3a signaling pathway molecules in the ovaries were assessed. Compared with control group, the weight of ovaries in both pZP3 group and pZP3‐Treg group was decreased and no difference was found between them. The number of follicles in the Treg transferred mice, like in pZP3 group, was significantly reduced compared to the control group, but showed a modest improvement when compared the pZP3 group alone. Significantly lower serum concentrations of follicle‐stimulating hormone, luteinizing hormone, and anti‐zona pellucida antibodies (AZPAbs) were found, while the concentrations of estradiol and anti‐Mullerian hormone increased. In mechanism, Treg cell transfer to ZP3 treated mice restored the levels of Caspase3 to control levels, and partially restored Bax, however, had no effect on Bcl‐2. Moreover, Treg cell transfer to ZP3 treated mice partially restored the levels of Akt and FOXO3a, and partially restored the ratios of p‐Akt/Akt and p‐FOXO3a/FOXO3a. In conclusion, Treg cells improved some aspects of ZP3‐induced POI which may be mediate by suppressing ovarian cells apoptosis and involving the Akt/FOXO3a signaling pathway. Therefore, Treg cells may be protective against autoimmune POI.

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supporting

confidence: 83%

“…The follicles were counted when the oocyte contained a clearly visible nucleus. Furthermore, the follicles were classified as primordial, primary, secondary, or atretic according to a method described previously (Hirshfield & Midgley, 1978;Tilly, 2003;Zhang et al, 2019).…”

Section: Hematoxylin and Eosin (Hande) Staining And Follicle Classifimentioning

confidence: 99%

Adoptive transfers of CD4⁺CD25⁺ Tregs partially alleviate mouse premature ovarian insufficiency

Liú

Chi-bing

et al. 2020

Molecular Reproduction Devel

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“…It can regulate the function of the female reproductive endocrine system, meanwhile, it stimulates the development of follicles in the ovary to restore ovarian reserve function and promote ovulation [6,7]. According to the research of Han Zhang [8], POF female mice treated by electroacupuncture were mated with male mice, and the final result was that the number of litters in the electroacupuncture group increased compared with the model group (p < 0.05), which indicated that acupuncture has the effect of protecting fertility. Acupuncture not only has obvious therapeutic effects without the side effects of estrogen, but also has the advantages of simplicity, convenience, low cost, and efficiency [9].…”

Section: Introductionmentioning

confidence: 99%

Acupuncture Reduces Apoptosis of Granulosa Cells in Rats with Premature Ovarian Failure Via Restoring the PI3K/Akt Signaling Pathway

Wang

Lin

Mingmin

et al. 2019

IJMS

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Acupuncture is widely recognized as an effective therapy for premature ovarian failure (POF) in clinical, but information about its potential mechanisms is rarely explored. To investigate the mechanism, fifty SD female rats were randomly divided into normal group, POF group, POF+estradiol-valerate group (abbreviated as estradiol group), and POF+acupuncture group (abbreviated as acupuncture group). The estrous cycle of the rats was tracked by vaginal smears. Their ovaries morphology was observed by hematoxylin-eosin staining. The apoptotic level of granulosa cells was detected by in situ TUNEL fluorescence staining assay. Serum follicle-stimulating hormone (FSH) and estrogen (E2) levels were measured by enzyme-linked-immunosorbent-assay (ELISA). Protein and gene expression of PI3K, Akt, bcl-2, and bax were detected by Western blotting and qPCR. In the acupuncture and estradiol groups, compared with the POF group as controls, the apoptosis number of granulosa cells was significantly decreased (p < 0.05). FSH levels were decreased, while E2 levels were increased (p > 0.05). The gene and protein expression levels of PI3K, Akt, and bcl-2 were increased, while the expression levels of bax were decreased (p < 0.05), and the protein expression level of p-Akt increased. There was no significant difference between the acupuncture group and the estradiol group (p > 0.05). Acupuncture was able to regulate hormone levels in POF rats, up-regulate PI3K/Akt signaling pathway, and reduce the apoptosis of granulosa cells. This may be one of the mechanisms of acupuncture treating premature ovarian failure.

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“…Advanced molecular and clinical studies have continually attempted to decipher the mechanisms underlying these effects of acupuncture [2,7,16,17,. The signal transduction pathways through which acupuncture treats nervous system diseases involves multiple signal pathways, including p38 mitogen-activated protein kinases (p38 MAPKs) [7, [33][34][35][36][37][38][39], Raf/MAPK/extracellular signal-regulated kinases (ERK) 1/2 [27][28][29][30][31][32]39], Toll-like receptor 4 (TLR4)/ERK [40][41][42][43]59], phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (Akt) [26,31,38,49], adenyl cyclase (AC)/cyclic-adenosine mono-phosphate (cAMP)/protein kinase A (PKA) [25,31,44,47,[50][51][52], apoptosis signal-regulating kinase 1 (ASK1)-c-Jun amino-terminal kinases (JNK)/p38 [7, [33][34][35][36][37][38][39]44,47,48,63], and down...…”

Section: Introductionmentioning

confidence: 99%

“…Acupuncture is widely used to treat various diseases and exerts positive effects, including analgesia, at both local and systemic levels [ 3 , 8 , 9 , 10 , 11 , 12 ]; it improves consciousness and cognition [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ], and it induces therapeutic effects in several nervous system diseases [ 8 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. Advanced molecular and clinical studies have continually attempted to decipher the mechanisms underlying these effects of acupuncture [ 2 , 7 , 16 , 17 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ,…”

Section: Introductionmentioning

confidence: 99%

Effect of Acupuncture on the p38 Signaling Pathway in Several Nervous System Diseases: A Systematic Review

Wei

Hsieh

2020

IJMS

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Acupuncture is clinically used to treat various diseases and exerts positive local and systemic effects in several nervous system diseases. Advanced molecular and clinical studies have continually attempted to decipher the mechanisms underlying these effects of acupuncture. While a growing understanding of the pathophysiology underlying several nervous system diseases shows it to be related to inflammation and impair cell regeneration after ischemic events, the relationship between the therapeutic mechanism of acupuncture and the p38 MAPK signal pathway has yet to be elucidated. This review discusses the latest advancements in the identification of the effect of acupuncture on the p38 signaling pathway in several nervous system diseases. We electronically searched databases including PubMed, Embase, and the Cochrane Library from their inception to April 2020, using the following keywords alone or in various combinations: “acupuncture”, “p38 MAPK pathway”, “signaling”, “stress response”, “inflammation”, “immune”, “pain”, “analgesic”, “cerebral ischemic injury”, “epilepsy”, “Alzheimer’s disease”, “Parkinson’s disease”, “dementia”, “degenerative”, and “homeostasis”. Manual acupuncture and electroacupuncture confer positive therapeutic effects by regulating proinflammatory cytokines, ion channels, scaffold proteins, and transcription factors including TRPV1/4, Nav, BDNF, and NADMR1; consequently, p38 regulates various phenomena including cell communication, remodeling, regeneration, and gene expression. In this review article, we found the most common acupoints for the relief of nervous system disorders including GV20, GV14, ST36, ST37, and LI4. Acupuncture exhibits dual regulatory functions of activating or inhibiting different p38 MAPK pathways, contributing to an overall improvement of clinical symptoms and function in several nervous system diseases.

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Electro-acupuncture attenuates the mice premature ovarian failure via mediating PI3K/AKT/mTOR pathway

Cited by 47 publications

References 24 publications

Adoptive transfers of CD4⁺CD25⁺ Tregs partially alleviate mouse premature ovarian insufficiency

Adoptive transfers of CD4⁺CD25⁺ Tregs partially alleviate mouse premature ovarian insufficiency

Acupuncture Reduces Apoptosis of Granulosa Cells in Rats with Premature Ovarian Failure Via Restoring the PI3K/Akt Signaling Pathway

Effect of Acupuncture on the p38 Signaling Pathway in Several Nervous System Diseases: A Systematic Review

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Electro-acupuncture attenuates the mice premature ovarian failure via mediating PI3K/AKT/mTOR pathway

Cited by 47 publications

References 24 publications

Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency

Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency

Acupuncture Reduces Apoptosis of Granulosa Cells in Rats with Premature Ovarian Failure Via Restoring the PI3K/Akt Signaling Pathway

Effect of Acupuncture on the p38 Signaling Pathway in Several Nervous System Diseases: A Systematic Review

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Adoptive transfers of CD4⁺CD25⁺ Tregs partially alleviate mouse premature ovarian insufficiency

Adoptive transfers of CD4⁺CD25⁺ Tregs partially alleviate mouse premature ovarian insufficiency