Electro-endocrine combination therapy for aggressive breast tumors

Camarillo, Ignacio G.; Nichols, Maxine B.; Zheng, Mary M.; Sonnenburg, Dan; Sundararajan, Raji

doi:10.1016/j.elstat.2007.08.010

Cited by 7 publications

(3 citation statements)

References 12 publications

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“…We used curcumin in the present study, as we had started investigating a number of commonly administered drugs, including tamoxifen, paclitaxel, doxorubicin, bleomycin, cisplatin, gemcitabine, and veliparib [17][18][19][20] using electric pulses (EPs) and various breast cancer cells since 2007. We have recently started studying curcumin due to its excellent anticancerous, anti-oxidant, and anti-bacterial qualities, as well as its effectiveness for treating several chronic conditions, including cancer [21].…”

Section: Introductionmentioning

confidence: 99%

Viability and cell cycle studies of metastatic triple negative breast cancer cells using low voltage electrical pulses and herbal curcumin

Mittal

Raman

Camarillo

et al. 2019

Biomed. Phys. Eng. Express

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Cancer is a disease of cell cycle, characterized by uncontrolled cell division. The tumor suppressor gene p53 plays a major role in cell cycle control, blocking the cell progression at G 1 /S phases. In triple negative breast cancer (TNBC), the lack/mutation of p53 is a common genetic irregularity that promotes survival signals leading to tumorigenesis. Due to its unique molecular profile, aggressive behavior, and metastasis, no targeted therapies exist for TNBC. This motivates our exploration of the influence of Electro-Curcumin-Therapy on the viability, cell cycle and p53 expression profile of TNBC cells. MDA-MB-231, human TNBC cells were treated with curcumin and eight 200 V cm −1 electric pulses (EPs) of either 100 μs or 5 ms duration. The synergy of 5 ms EPs and curcumin reduced viability to as low as 6% at 48 h after treatment. Combining the 100 μs EPs with curcumin arrested 80% cells in the G 0 /G 1 phase. Applying 5 ms EPs with curcumin caused cells to shift from the G 2 phase (9% reduction) to the S phase population (21% increase) compared to control. Combining 100 μs EPs with curcumin significantly downregulated the p53 level, while the p53 level significantly increased for 5 ms EPs with and without curcumin. These results indicate the potent effect of combining EPs and curcumin to effectively cause cell death in TNBC cells with different mechanistic behaviors, while having a much-reduced impact on cell death in normal mammary cells.

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Section: Introductionmentioning

confidence: 99%

Viability and cell cycle studies of metastatic triple negative breast cancer cells using low voltage electrical pulses and herbal curcumin

Mittal

Raman

Camarillo

et al. 2019

Biomed. Phys. Eng. Express

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“…Moreover, clinical trials are underway for the delivery of drugs into the skin [7]. In our previous work, we successfully delivered the hormone drug Tamoxifen using electroporation with 200 V/cm, 10-40ms, 8 pulses at one second intervals [8]. The same intensity was used in our initial effort in this study.…”

Section: Electroporation Techniquementioning

confidence: 97%

Impedance spectroscopy studies of electroporated human breast cancer cells

Xiao¹,

Otto²,

Camarillo³

et al. 2009

2009 IEEE Conference on Electrical Insulation and Dielectric Phenomena

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The passive, electrical impedance characteristics of electroporated, MCF-7 human breast cancer is described in this paper. For this purpose, an Autolab impedance spectroscope was used. The electroporation protocol consisted of 200V/cm, 40ms, and 1200V/cm, 200 s pulses. The impedance characteristics of these cells displayed a negative fractal power law as expected for dielectrics. They also exhibited single dispersion Cole-Cole model.Our experiments demonstrated that the various electroporation treatment parameters result in the different curvatures of Cole-Cole model. This phenomenon proves that the effects of electroporation drug delivery to cells could be controlled in real time by monitoring impedance characteristics.

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“…The electroparameters included 200 v/cm, 20 ms and 40 ms pulses. Recent results suggest that applications of electrical pulses along with chemo treatment to breast cancer cells grown in culture enhanced the drug transport across the plasma membranes (Camarillo et al 2008 ) . However, the method will have limitations when one would like to apply it to the breast cancer patients.…”

Section: Electrically Enhanced Chemo-drug Delivery To Human Breast Camentioning

confidence: 99%

Apoptosis of Breast Cancer Cells: Modulation of Genes for Glycoconjugate Biosynthesis and Targeted Drug Delivery

Basu

Moskal³

et al. 2012

Advances in Experimental Medicine and Biology

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Electro-endocrine combination therapy for aggressive breast tumors

Cited by 7 publications

References 12 publications

Viability and cell cycle studies of metastatic triple negative breast cancer cells using low voltage electrical pulses and herbal curcumin

Viability and cell cycle studies of metastatic triple negative breast cancer cells using low voltage electrical pulses and herbal curcumin

Impedance spectroscopy studies of electroporated human breast cancer cells

Apoptosis of Breast Cancer Cells: Modulation of Genes for Glycoconjugate Biosynthesis and Targeted Drug Delivery

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