Electro-immunoassay for properdin

Babesia rodhaini : Requirement of Complement for Penetration of Human Erythrocytes

“…The serum eluate (RB) was pooled, dialyzed twice against a 200-volume excess of VBS at 4°C, centrifuged at 20,000 rev/min for 30 minutes at 40C, and stored frozen at -70°C in small portions. No factor B was detected in the RB preparation by the Laurell rocket technique which was conducted by appropriate modification (antibody concentration and field polarity) of the method described elsewhere (14) and by immunoelectrophoresis (15). As expected, zymosan (5 mg/ml, 37°C, 45 minutes) activation of the alternative pathway occurred only in factor B-recon-stituted RB.…”

“…Classical complement components were unaffected by this procedure because essentially similar CH50 values (where CH50 is the amount of complement that lyses 50 percent of a standardized number of sensitized red cells) were obtained in RB and in a sample of normal human serum treated similarly except for the omission of the affinity column step (pseudo RB). The RB and "pseudo RB" contained similar concentrations of properdin as assessed by the Laurell technique (14).…”

“…In the past 30 years, cutting oils have been frequently cited as related to cancer of the scrotum among machine operators (14,15). Several cutting oils were found to be carcinogenic to laboratory animals (15,16), and compounds such as polycyclic aromatic hydrocarbons and heterocyclic compounds were believed to be the carcinogens (17).…”