Electro-optical recording system for myocardial ischemia studies in animal experiments

Provazník, Ivo; Novàkovâ, Marie; Vesely, Z.; Blaha, Mojmír; Chmelař, Milan

doi:10.1109/cic.2003.1291220

Cited by 8 publications

(4 citation statements)

References 2 publications

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“…The methods used in the projects are based on animal experiments in which MAP is recorded from isolated rabbit heart perfused according to Langendorff. MAP was recorded by simple optical method [2] and by new proposed method. MAPs are compared by signal-to-noise ratio (SNR).…”

Section: Methodsmentioning

confidence: 99%

“…There are two different setups for MAP measurement used in this project, simple optical setup [2] and new advanced ratiometric setup.…”

Section: Recording Setupmentioning

confidence: 99%

“…They consist of three sensors in a single package, each of them sensitive in non-overlapping wavelength bands. A new method is compared with simple optical MAPs measurement [2].…”

Section: Introductionmentioning

confidence: 99%

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New recording setup for ratiometric recording of action potentials by optical means

Bardoňová

Provazník

Novàkovâ

et al. 2008

2008 Computers in Cardiology

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Monophasic action potential (MAP) can be recorded from the heart surface by optical method via fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which a new ratiometric recording setup is used for recording MAPs from isolated rabbit hearts perfused according to Langendorff. MAPs recorded by new ratiometric recording setup are compared with simple optical MAP measurement without ratiometry.

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Section: Methodsmentioning

confidence: 99%

“…There are two different setups for MAP measurement used in this project, simple optical setup [2] and new advanced ratiometric setup.…”

Section: Recording Setupmentioning

confidence: 99%

See 1 more Smart Citation

New recording setup for ratiometric recording of action potentials by optical means

Bardoňová

Provazník

Novàkovâ

et al. 2008

2008 Computers in Cardiology

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“…The employed optical recording system is based on application of voltage sensitive dye di-4-ANEPPS (Molecular Probes, USA) into the examined tissue [7]. The dye undergo changes in its fluorescence spectra, in response to changes in the surrounding electric field.…”

Section: Methodsmentioning

confidence: 99%

Analysis of dynamic changes in ECG signals during optical mapping by dynamic time warping

Vyklický

Bardoňová

Provazník

et al. 2005

Computers in Cardiology, 2005

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Voltage-sensitive dyes (VSDs) IntroductionIschemic heart disease (or coronary h.d.), the most common cause of death in the industrialized countries [1], covers a range of conditions in which the blood supply to the heart muscle, and consequently the heart functions, become limited. As a rule, it is caused by a narrowing of a branch of the coronary artery (stenosis) by atherosclerotic process.The diagnostic processes of ischemic heart disease consist of invasive and non-invasive methods. The development of non-invasive methods has been required by the tendency to avoid the untoward side effects during instrumentation, risk of iatropathogenic damage (mainly by infection), and last but not least, the economic aspects of cardiovascular diagnostics. Further, the results could help to develop a new noninvasive method needed in cardiology diagnostics.Ischemic heart disease is instrumentally diagnosed by coronary angiography, which represents the reference method. Echocardiographic data offer valuable information on regional or global impairment of cardiac function as do the radionuclid perfusion methods based on thallium 201 or technetium 99 uptake. For more than a half of a century, the most widely used diagnostic test for the detection and evaluation of cardiac ischemia are the electrocardiographic method [2]. The attenuated or stopped coronary perfusion causes a potential difference between the ischemic and normal regions during the ST segment and, eventually, also at rest. While ST depression is the common manifestation of transitory, exercise-induced cardiac ischemia, ST elevation is related to severe transmural ischemia, acute myocardial infarction. Myocardial ischemia affects also other features than the ST segment [3] including an increase of QRS amplitude, subtle prolongation of QRS duration and shifts of QRS axis [4]. These changes reflect abnormal conduction due to irregular membrane depolarization. There is ample evidence that cardiac ischemia may change the QRS spectrum as the expression of fragmentation of ventricular depolarization.The recent findings show that acute myocardial ischemia in early stages can be detected using the analysis of intra-QRS changes. These changes reveal local ischemia-induced propagation changes earlier than traditional ECG-based indexes [5], [6]. Recent findings specify the changes as low-amplitude short-time events within a window of ventricular depolarization. The actual time and frequency localization of the changes depend on the heart status.Myocardial ischemia studies using optical mapping consists of four experimental phases: control period, dye loading, wash-out, myocardial ischemia. The use of VSDs may negatively influenced electrophysiology of the examined heart. Therefore, dynamic changes in electrocardiograms during particular phases of the experiment must be studied.A number of method based on deterministic or stochastic theory can be applied.

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Influence of ischemia on left ventricular depolarization in isolated rabbit hearts perfused according to Langendorff

et al. 2009

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Electro-optical recording system for myocardial ischemia studies in animal experiments

Cited by 8 publications

References 2 publications

New recording setup for ratiometric recording of action potentials by optical means

New recording setup for ratiometric recording of action potentials by optical means

Analysis of dynamic changes in ECG signals during optical mapping by dynamic time warping

Influence of ischemia on left ventricular depolarization in isolated rabbit hearts perfused according to Langendorff

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