Electroacupuncture Improves Insulin Sensitivity in High-Fat Diet-Induced Insulin Resistant Rats by Activating SIRT1 and GLUT4 in Quadriceps Femoris

Xu, Fen; Chen, R; Ma, Cary; Tang, Lin-Xia; Wan, Wei; You, Fu-Nian; Chen, L; Li, J; Z, Chen; Liang, Feng

doi:10.4183/aeb.2020.280

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…EA can correct various metabolic disorders such as overweight, excessive appetite [55], hyperlipidemia [56], and inflammation [57], all of which contribute to the development of IR [54, 58]. The combination of EA at acupoints ST36, CV4, CV12, and ST40 has been shown to improve HFDI obesity and IR [59]. Unfortunately, the neuronal networks that mediate the effects of EA were not further explored in this study.…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture Alleviates Obesity and Insulin Resistance via the GLP-1-VTADA Reward Circuit

Zhu,

Tian,

Wei

et al. 2023

Neuroendocrinology

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Introduction: We investigated the effects of electroacupuncture (EA) on improving obesity and insulin resistance (IR) in high-fat diet-induced (HFDI) obese rats by modulating the nucleus tractus solitarius (NTS) glucagon-like peptide-1 (GLP-1)-ventral tegmental area (VTA) dopamine (DA) neural reward circuit, thereby uncovering a possible central mechanism underlying EA’s actions in improving obesity and IR. Methods: We randomly allocated 45 Wistar male rats to five groups (normal, model, EA, chemogenetic activation, chemogenetic suppression + EA), with 9 rats in each group. All interventions were conducted within 8 weeks after the model was established. We tested rats for obesity phenotypes included body mass, Lee’s index, 24-h food intake, and glucose-metabolism parameters. We observed protein and gene expression for GLP-1 in the NTS and tyrosine hydroxylase in the VTA by Western blotting and real-time polymerase chain reaction, as well as their localization by immunofluorescence. We also determined the DA content in the VTA using high-performance liquid chromatography. Results: Obese rats exhibited marked hyperphagia, accompanied by increased excitability of DA neurons in the VTA region and reduced insulin sensitivity. After EA treatment, obese rats showed augmented excitability of NTS GLP-1 and suppression of VTADA neurons with a diminution in food intake, showing results similar to those in the chemogenetic activation group. After EA treatment and while inhibiting GLP-1 neurons by chemogenetics, the effect of EA on activating GLP-1 neurons and inhibiting VTADA was partially abrogated. The effects of improving obesity and insulin sensitivity were likewise also suppressed. Conclusion: EA effectively activated GLP-1 neurons in the NTS, thereby inhibited the expression of DA in the VTA and improved obesity and insulin sensitivity in HFDI-obese rats.

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Section: Discussionmentioning

confidence: 99%

Electroacupuncture Alleviates Obesity and Insulin Resistance via the GLP-1-VTADA Reward Circuit

Zhu,

Tian,

Wei

et al. 2023

Neuroendocrinology

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“…Additionally, Sirt-1 overexpression protects against metabolic abnormalities via high-fat diets and insulin resistance in diabetic animals. Sirt-1 controls several transcription factors in endocrine signaling pathways, most notably glucose transporter-4 (GLUT-4), therefore improving insulin sensitivity [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Obesity Effect of a Tea Mixture Nano-Formulation on Rats Occurs via the Upregulation of AMP-Activated Protein Kinase/Sirtuin-1/Glucose Transporter Type 4 and Peroxisome Proliferator-Activated Receptor Gamma Pathways

et al. 2023

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White, green, and oolong teas are produced from the tea plant (Camellia sinensis (L.) Kuntze) and are reported to have anti-obesity and hypolipidemic effects. The current study aims to investigate the anti-obesity effects of a tea mixture nano-formulation by targeting the AMPK/Sirt-1/GLUT-4 axis in rats. In vitro lipase and α-amylase inhibition assays were used to determine the active sample, which was then incorporated into a nanoparticle formulation subjected to in vivo anti-obesity testing in rats by measuring the expression level of different genes implicated in adipogenesis and inflammation using qRT-PCR. Moreover, metabolomic analysis was performed for each tea extract using LC/ESI MS/MS coupled to chemometrics in an attempt to find a correlation between the constituents of the extracts and their biological activity. The in vitro pancreatic lipase and α-amylase inhibition assays demonstrated more effective activity in the tea mixture than the standards, orlistat and acarbose, respectively, and each tea alone. Thus, the herbal tea mixture and its nanoparticle formulation were evaluated for their in vivo anti-obesity activity. Intriguingly, the tea mixture significantly decreased the serum levels of glucose and triglycerides and increased the mRNA expression of GLUT-4, P-AMPK, Sirt-1, and PPAR-γ, which induce lipolysis while also decreasing the mRNA expression of TNF-α and ADD1/SREBP-1c, thereby inhibiting the inflammation associated with obesity. Our study suggests that the tea mixture nano-formulation is a promising therapeutic agent in the treatment of obesity and may also be beneficial in other metabolic disorders by targeting the AMPK/Sirt-1/Glut-4 pathway.

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Electroacupuncture Improves Insulin Sensitivity in High-Fat Diet-Induced Insulin Resistant Rats by Activating SIRT1 and GLUT4 in Quadriceps Femoris

Cited by 2 publications

References 34 publications

Electroacupuncture Alleviates Obesity and Insulin Resistance via the GLP-1-VTA<sup>DA</sup> Reward Circuit

Electroacupuncture Alleviates Obesity and Insulin Resistance via the GLP-1-VTA<sup>DA</sup> Reward Circuit

Anti-Obesity Effect of a Tea Mixture Nano-Formulation on Rats Occurs via the Upregulation of AMP-Activated Protein Kinase/Sirtuin-1/Glucose Transporter Type 4 and Peroxisome Proliferator-Activated Receptor Gamma Pathways

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