Electroanalytical devices with pins and thread

Glavan, Ana C; Ainla, Alar; Hamedi, Mahiar; Fernández‐Abedul, M. Teresa; Whitesides, George M.

doi:10.1039/c5lc00867k

Cited by 54 publications

(34 citation statements)

References 51 publications

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“…A number of studies have detailed their application in clinical diagnostics, pharmaceutical analysis, environmental monitoring, and food quality. A variety of analytes have been examined including small‐molecule metabolites, nucleic acids, proteins, and metal ions .…”

Section: Introductionmentioning

confidence: 99%

A microfluidic glucose sensor incorporating a novel thread‐based electrode system

et al. 2018

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An electrochemical sensor for the detection of glucose using thread-based electrodes and fabric is described. This device is relatively simple to fabricate and can be used for multiple readings after washing with ethanol. The fabrication of the chip consisted of two steps. First, three thread-based electrodes (reference, working, and counter) were fabricated by painting pieces of nylon thread with either layered silver ink and carbon ink or silver/silver chloride ink. The threads were then woven into a fabric chip with a beeswax barrier molded around the edges in order to prevent leaks from the tested solutions. A thread-based working electrode consisting of one layer of silver underneath two layers of carbon was selected to fabricate the final sensor system. Using the chip, a PBS solution containing glucose oxidase (GOx) (10 mg/mL), potassium ferricyanide (K [Fe(CN) ]) (10 mg/mL) as mediator, and different concentrations of glucose (0-25 mM), was measured by cyclic voltammetry (CV). It was found that the current output from the oxidation of glucose was proportional to the glucose concentrations. This thread-based electrode system is a viable sensor platform for detecting glucose in the physiological range.

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Section: Introductionmentioning

confidence: 99%

A microfluidic glucose sensor incorporating a novel thread‐based electrode system

et al. 2018

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“…At present, there are fewer thread‐based devices that have incorporated electrochemical detection than paper . Typical methods of fabricating electrodes on either thread or paper‐based devices include screen or stencil printing, or chemical vapor deposition as well as screens, masks, stencils, and conductive materials .…”

Section: Introductionmentioning

confidence: 99%

Microfluidic thread‐based electrode system to detect glucose and acetylthiocholine

et al. 2018

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A reusable and simple to fabricate electrochemical sensor for the detection of glucose and acetylthiocholine using thread‐based electrodes and nylon thread is described. The fabrication of the device consisted of two steps. First, three nylon‐based electrodes (reference, working, and counter) were painted with one layer of conductive inks (silver and carbon ink, or silver/silver chloride ink). The electrodes were taped onto parafilm, and a piece of white nylon thread was wrapped around each electrode connecting the three electrodes. For the glucose system, a PBS solution containing glucose oxidase (GOx) (10 mg/mL), and potassium ferricyanide (K3[Fe(CN)6]) (10 mg/mL) as mediator, was dried onto the thread, and increasing concentrations of glucose (0–15 mM) was added to the thread and measured by cyclic voltammetry (CV). The current output from the glucose oxidation was proportional to the concentration of glucose. For the second system, a solution of acetylcholinesterase (AChE) (0.08 U/mL) in PBS was added to the nylon thread, and increasing concentrations of acetylthiocholine (ATC) (0–9.84 mg/mL) was added and measured by CV. The current output from the oxidation of thiocholine (produced by AChE reacting with ATC) was proportional to the concentrations of ATC added to the thread. From both systems, a graph of current output versus substrate concentration was produced and fitted with a linear regression line that gave R2 values of 0.985 (GOX/glucose) and 0.995 (AChE/ATC).

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“…In this work, modification with carbon nanotubes was considered since they have demonstrated to improve the analytical signal (Fanjul-Bolado et al, 2007b;Fernández-Abedul and Costa-García, 2008) and have been employed in the first electroanalytical pin-application (Glavan et al, 2016). Apart from the composition of the ink, the time of drying is also important.…”

Section: Evaluation Of the Coating Of The Pins With Carbon Inkmentioning

confidence: 99%

“…Pins provide a simple solution to the challenge of fabrication and integration of electrodes in miniaturized devices. Their use has only been reported previously in a work made in collaboration with Whitesides group (Glavan et al, 2016). We used them in systems fabricated on omniphobic R F paper or thread to quantify lactate in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Pin-based electrochemical glucose sensor with multiplexing possibilities

Costa‐Rama

Costa-Garcı́a

Fernández‐Abedul

2017

Biosensors and Bioelectronics

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This work describes the use of mass-produced stainless-steel pins as low-cost electrodes to develop simple and portable amperometric glucose biosensors. A potentiostatic three-electrode configuration device is designed using two bare pins as reference and counter electrodes, and a carbon-ink coated pin as working electrode. Conventional transparency film without any pretreatment is used to punch the pins and contain the measurement solution. The interface to the potentiostat is very simple since it is based on a commercial female connection. This electrochemical system is applied to glucose determination using a bienzymatic sensor phase (glucose oxidase/horseradish peroxidase) with ferrocyanide as electron-transfer mediator, achieving a linear range from 0.05 to 1mM. It shows analytical characteristics comparable to glucose sensors previously reported using conventional electrodes, and its application for real food samples provides good results. The easy modification of the position of the pins allows designing different configurations with possibility of performing simultaneous measurements. This is demonstrated through a specific design that includes four pin working-electrodes. Different concentrations of antibody labeled with alkaline phosphatase are immobilized on the pin-heads and after enzymatic conversion of 3-indoxylphosphate and silver nitrate, metallic silver is determined by anodic stripping voltammetry.

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Electroanalytical devices with pins and thread

Cited by 54 publications

References 51 publications

A microfluidic glucose sensor incorporating a novel thread‐based electrode system

A microfluidic glucose sensor incorporating a novel thread‐based electrode system

Microfluidic thread‐based electrode system to detect glucose and acetylthiocholine

Pin-based electrochemical glucose sensor with multiplexing possibilities

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