Electrochemical aptasensor based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling for lipopolysaccharide detection

Xie, Shunbi; Liang, Shuting; Tian, Liangliang; Ding, Ge; He, Meiting; Li, Haojie; Yang, Heshan

doi:10.1039/d3ay01638b

Anal. Methods

2024

DOI: 10.1039/d3ay01638b

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Electrochemical aptasensor based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling for lipopolysaccharide detection

Shunbi Xie,

Shuting Liang,

Liangliang Tian

et al.

Abstract: An electrochemical aptasensor for detecting lipopolysaccharides was fabricated based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling.

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Aptasensors and Advancement in Molecular Recognition Technology

Napit,

Jaysawal,

Chowdhury

et al. 2024

Adv Materials Technologies

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Synthetic oligonucleic acids known as aptamers exhibit remarkable selectivity and affinity for target recognition and binding. Selected via an iterative process known as “selective evolution of ligands by exponential enrichment” (SELEX), aptamers fold into defined 3D conformations to interact with their targets. The incorporation of aptamers as recognition elements has driven notable progress in biosensors, giving rise to the development of aptasensors. Here, the process of aptamer discovery and the development of various types of aptasensors are summarized. The fundamental design principles of aptasensors are elaborated along with the superiority of aptamers compared to antibodies. The various modes employed by aptasensors, such as structure‐switching design, hybridization chain reaction amplification, enzyme‐assisted recycling, and split aptamer design are examined. Further light is shed on the diverse landscape of aptasensors, their adaptability to different analytes aptasensors as well as their potential to propel advancements in modern biosensor technology. As a nucleic acids‐based biosensor platform, aptasensors poise to become a next generation of sensitive and cost‐effective technology to shape the future of molecular recognition in biosensing.

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Aptasensors and Advancement in Molecular Recognition Technology

Napit,

Jaysawal,

Chowdhury

et al. 2024

Adv Materials Technologies

View full text Add to dashboard Cite

show abstract

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Electrochemical aptasensor based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling for lipopolysaccharide detection

Abstract: An electrochemical aptasensor for detecting lipopolysaccharides was fabricated based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling.

Cited by 1 publication

References 37 publications

Aptasensors and Advancement in Molecular Recognition Technology

Aptasensors and Advancement in Molecular Recognition Technology

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