Electrochemical aptasensor based on proximity binding-induced DNA networked for enzyme-free and ultrasensitive detection of thrombin

Li, Wei; Zhao, Dingxin; Zhai, Manman; Xu, Hongdan; Zheng, Liu-Chuang; Li, Shengqiang; Sang, Ye

doi:10.1016/j.jelechem.2021.115447

Cited by 8 publications

(7 citation statements)

References 36 publications

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“…as proposed by Gooding´s Group, [7,21,22,45] and for amplification using more conventional electrochemical biosensor formats. [23,24] The key to "dispersible electrodes" is the use of Au-coated MNPs (Au@MNPs), modified with the appropriate receptor to selectively recognize the analyte, combining the ability to move the NPs with a magnetic field with electrical conductivity. This strategy showed a 1000-fold improvement in both detection limit and response time compared to the same sensing elements in macroscopic and planar electrochemical sensors.…”

Section: Dna Network and Hydrogelsmentioning

confidence: 99%

“…DNA networks generated by DNA probes functionalized with biotin at both ends [23] or by non-linear HCR amplification strategies [24,46] (Figure 6b) have been exploited to develop biosensors with improved sensitivity. Representative examples of [22] Copyright 2021, RSC; (b) Reproduced with permission.…”

Section: Dna Network and Hydrogelsmentioning

confidence: 99%

“…these concepts are the enzyme-free biosensors reported by Liu et al for the determination of telomerase activity and by Li et al for thrombin. [23,24] DNA hydrogels are 3D porous network polymers containing a large amount of water and constructed by crosslinking only nucleic acids (pure DNA hydrogels) or grafting DNA strands on hydrophilic polymers or other materials (hybrid DNA hydrogels). [26] Pure DNA hydrogels have limited mechanical properties and high cost compared to the hybrid ones.…”

Section: Dna Network and Hydrogelsmentioning

confidence: 99%

“…DNA networks have been exploited to improve sensitivity and reduce assay time through the concept of using conductive gold‐coated magnetic nanoparticles as “dispersible electrodes.” as proposed by Gooding´s Group, [ 7,21,22,45 ] and for amplification using more conventional electrochemical biosensor formats. [ 23,24 ]…”

Section: Nanostructured and Multifunctional Dna Biomaterialsmentioning

confidence: 99%

mentioning

confidence: 99%

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Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

Campuzano

Pedrero

Barderas

et al. 2022

Adv Materials Technologies

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Electrochemical biosensors continue to evolve at an astonishing pace, consolidating as competitive tools for determining a wide range of targets and relentlessly strengthening their attributes in terms of sensitivity, selectivity, simplicity, response time, and antifouling ability, making them suitable for getting a foothold in real‐world applications. The design and exploitation of nanostructured or multifunctional nucleic acid or peptide biomaterials are playing a determinant role in these achievements. With the aim of highlighting the potential and opportunities of these biomaterials, this perspective article critically discusses and overviews the electrochemical biosensors reported since 2019 involving nanostructured and multifunctional DNA biomaterials, multifunctional aptamers, modern peptides, and CRISPR/Cas systems. The use of these biomaterials as recognition elements, electrode modifiers (acting as linkers or creating scaffolds with antifouling properties), enzyme substrates, and labeling/carrier agents for signal amplification is discussed through rationally and strategically selected examples, concluding with a personal perspective about the challenges to be faced and future lines of action.

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Section: Dna Network and Hydrogelsmentioning

confidence: 99%

Section: Dna Network and Hydrogelsmentioning

confidence: 99%

Section: Dna Network and Hydrogelsmentioning

confidence: 99%

Section: Nanostructured and Multifunctional Dna Biomaterialsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

Campuzano

Pedrero

Barderas

et al. 2022

Adv Materials Technologies

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Incorporation of a Multi‐Valent Aptamer into Electrochemical Biosensors to Achieve an Improved Performance for Thrombin Analysis in Blood Serum

Zhu

Miao

et al. 2022

ChemPlusChem

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The electrochemical aptamer‐based (E‐AB) biosensor usually has a long reaction time when detecting thrombin. This work reports the design of an E‐AB biosensor with dual recognition sites to quickly detect thrombin. Specifically, two specific recognition sites of thrombin were used to design three aptamer sequences (TBA‐15, TBA‐29 and TBA‐U), followed by fabrication of corresponding sensors. First, we tested these three types of biosensors in tris buffer solution, and found that the response time of the TBA‐U sensor to the same concentration of thrombin was about 2 hours, which is shorter than TBA‐15 and TBA‐29 sensors. Then, we also did the same test in 50 % diluted serum with 500 nM thrombin. The response time of the TBA‐U sensor was about 2 hours, which is still faster than the 3 hours of TBA‐15 sensor and the 5.5 hours for TBA‐29 sensor. In addition, in terms of dynamic range and specificity, TBA‐U has good performance.

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Flower-like titanium dioxide as novel co-reaction accelerator for ultrasensitive “off–on” electrochemiluminescence aptasensor construction based on 2D g-C3N4 layer for thrombin detection

Lin

Tan

et al. 2022

J Solid State Electrochem

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Electrochemical aptasensor based on proximity binding-induced DNA networked for enzyme-free and ultrasensitive detection of thrombin

Cited by 8 publications

References 36 publications

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

Incorporation of a Multi‐Valent Aptamer into Electrochemical Biosensors to Achieve an Improved Performance for Thrombin Analysis in Blood Serum

Flower-like titanium dioxide as novel co-reaction accelerator for ultrasensitive “off–on” electrochemiluminescence aptasensor construction based on 2D g-C3N4 layer for thrombin detection

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