Electrochemical aptasensor for analyzing alpha-fetoprotein using RGO–CS–Fc nanocomposites integrated with gold–platinum nanoparticles

Li, Wenzhan; Chen, Min; Liang, Jianwei; Lu, Chunting; Zhang, Min; Hu, Fangrong; Zhou, Zhide; Li, Guiyin

doi:10.1039/d0ay01465f

Cited by 17 publications

(14 citation statements)

References 46 publications

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“…The lack of significant peaks at higher binding energy proved the absence of any oxidized gold species . The signal of Au 4f also significantly decreased after the electrode surface was immobilized with aptamers . Moreover, the rise of the S 2p peak at 162.9 eV in Figure h indicated a strong presence of Au–S bonds .…”

Section: Resultsmentioning

confidence: 95%

Aptamer-Based Electrochemical Microfluidic Biosensor for the Detection of Cryptosporidium parvum

et al. 2023

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Cryptosporidium parvum is a high-risk and opportunistic waterborne parasitic pathogen with highly infectious oocysts that can survive harsh environmental conditions for long periods. Current state-of-the-art methods are limited to lengthy imaging and antibody-based detection techniques that are slow, labor-intensive, and demand trained personnel. Therefore, the development of new sensing platforms for rapid and accurate identification at the point-of-care (POC) is essential to improve public health. Herein, we propose a novel electrochemical microfluidic aptasensor based on hierarchical 3D gold nano-/ microislands (NMIs), functionalized with aptamers specific to C. parvum. We used aptamers as robust synthetic biorecognition elements with a remarkable ability to bind and discriminate among molecules to develop a highly selective biosensor. Also, the 3D gold NMIs feature a large active surface area that provides high sensitivity and a low limit of detection (LOD), especially when they are combined with aptamers,. The performance of the NMI aptasensor was assessed by testing the biosensor's ability to detect different concentrations of C. parvum oocysts spiked in different sample matrices, i.e., buffer, tap water, and stool, within 40 min detection time. The electrochemical measurements showed an acceptable LOD of 5 oocysts mL −1 in buffer medium, as well as 10 oocysts mL −1 in stool and tap water media, over a wide linear range of 10−100,000 oocysts mL −1 . Moreover, the NMI aptasensor recognized C. parvum oocysts with high selectivity while exhibiting no significant cross-reactivity to other related coccidian parasites. The specific feasibility of the aptasensor was further demonstrated by the detection of the target C. parvum in patient stool samples. Our assay showed coherent results with microscopy and real-time quantitative polymerase chain reaction, achieving high sensitivity and specificity with a significant signal difference (p < 0.001). Therefore, the proposed microfluidic electrochemical biosensor platform could be a stepping stone for the development of rapid and accurate detection of parasites at the POC.

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Section: Resultsmentioning

confidence: 95%

Aptamer-Based Electrochemical Microfluidic Biosensor for the Detection of Cryptosporidium parvum

et al. 2023

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“…However, there is still concern about their toxicity for both humans and the environment [ 14 ], so most of their cancer-related applications are limited to their use in the development of sensors for cancer biomarkers. Their high surface area and excellent electrical conductivity [ 15 ] make them suitable candidates for the development of the electrochemical sensors used for the detection of biomarkers that are expressed in HCC [ 16 , 17 , 18 ].…”

Section: Types Of Nanoparticles Used For Diagnosis and Treatmentmentioning

confidence: 99%

“…Until then, AuNPs and other metallic nanoparticles remain valuable candidates for diagnostic techniques, such as the electrochemical detection of different biomarkers. In the context of HCC, gold nanoparticles [ 17 ], gold–platinum [ 16 ], or platinum–palladium nanostructures [ 18 ] have been used in conjunction with carbon-based nanomaterials, for the detection of biomarkers such as AFP or glypican-3. Gold nanostructures can, also, be employed for the imaging and photothermal ablation of tumors [ 27 ].…”

Section: Types Of Nanoparticles Used For Diagnosis and Treatmentmentioning

confidence: 99%

“…An AFP sensing technique uses an electrochemical method and an AFP aptamer for detection [ 16 ]. Aptamers are short, single-stranded nucleic acids that can bind to specific targets [ 45 ].…”

Section: Nanoparticles Used For Diagnosticsmentioning

confidence: 99%

“…Lastly, the aptamer was added to ensure selectivity towards AFP. The method showed some interferences with other proteins, but its sensitivity was sufficient for the detection of low concentrations of AFP [ 16 ].…”

Section: Nanoparticles Used For Diagnosticsmentioning

confidence: 99%

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Nanotechnology in the Diagnostic and Therapy of Hepatocellular Carcinoma

et al. 2022

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Hepatocellular carcinoma is the most common liver malignancy and is among the top five most common cancers. Despite the progress of surgery and chemotherapy, the results are often disappointing, in part due to chemoresistance. This type of tumor has special characteristics that allow the improvement of diagnostic and treatment techniques used in clinical practice, by combining nanotechnology. This article presents a brief review of the literature focused on nano-conditioned diagnostic methods, targeted therapy, and therapeutic implications for the pathology of hepatocellular carcinoma. Within each subdomain, several modern technologies with significant impact were highlighted: serological, imaging, or histopathological diagnosis; intraoperative detection; carrier-type nano-conditioned therapy, thermal ablation, and gene therapy. The prospects offered by nanomedicine will strengthen the hope of more efficient diagnoses and therapies in the future.

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Designing Nanosheet Heterostructures of CuO Grown on Bi₂MoO₆ as a Photoelectrochemical Biosensor for Detecting Alpha‐Fetoprotein

Zhao¹,

Yang

Zhao

et al. 2022

ChemElectroChem

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Bi 2 MoO 6 (BM) was modified with various amounts of CuO. Photoelectrochemical (PEC) tests of the resulting CuO-doped BM materials showed improved PEC performance. The best PEC performance was obtained for BM containing 5 wt % of CuO. CuO/BM-5 % was then used as an active material for an Alphafetoprotein (AFP) PEC biosensor. A PEC biosensor-based CuO/ BM-5 % detected AFP linearly from 0.01-1200 ng • L À 1 with a detection limit of 0.005 ng • L À 1 . The photoelectron potential of the CuO/BM-5 % heterojunction reduced the electron/hole recombination, which explains the excellent PEC performance of this material.

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Electrochemical aptasensor for analyzing alpha-fetoprotein using RGO–CS–Fc nanocomposites integrated with gold–platinum nanoparticles

Abstract: Electrochemical aptasensor for analyzing AFP using RGO–CS–Fc nanocomposites integrated with gold–platinum nanoparticles and AFP aptamer has been developed. The AFP aptasensor showed satisfactory selectivity, reproducibility and stability.

Cited by 17 publications

References 46 publications

Aptamer-Based Electrochemical Microfluidic Biosensor for the Detection of Cryptosporidium parvum

Aptamer-Based Electrochemical Microfluidic Biosensor for the Detection of Cryptosporidium parvum

Nanotechnology in the Diagnostic and Therapy of Hepatocellular Carcinoma

Designing Nanosheet Heterostructures of CuO Grown on Bi₂MoO₆ as a Photoelectrochemical Biosensor for Detecting Alpha‐Fetoprotein

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Electrochemical aptasensor for analyzing alpha-fetoprotein using RGO–CS–Fc nanocomposites integrated with gold–platinum nanoparticles

Abstract: Electrochemical aptasensor for analyzing AFP using RGO–CS–Fc nanocomposites integrated with gold–platinum nanoparticles and AFP aptamer has been developed. The AFP aptasensor showed satisfactory selectivity, reproducibility and stability.

Cited by 17 publications

References 46 publications

Aptamer-Based Electrochemical Microfluidic Biosensor for the Detection of Cryptosporidium parvum

Aptamer-Based Electrochemical Microfluidic Biosensor for the Detection of Cryptosporidium parvum

Nanotechnology in the Diagnostic and Therapy of Hepatocellular Carcinoma

Designing Nanosheet Heterostructures of CuO Grown on Bi2MoO6 as a Photoelectrochemical Biosensor for Detecting Alpha‐Fetoprotein

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Designing Nanosheet Heterostructures of CuO Grown on Bi₂MoO₆ as a Photoelectrochemical Biosensor for Detecting Alpha‐Fetoprotein