Electrochemical biomimetic enzyme cascade amplification combined with target-induced DNA walker for detection of thrombin

Hairpin-Empowered Invasive Reaction Combined with Catalytic Hairpin Assembly Cascade Amplification for the Specific Detection of Single-Nucleotide Polymorphisms

“…In general, increasing the sensitivity of a detection strategy often requires a combination of signal amplification techniques. Over the past few decades, a variety of signal amplification methods have been proposed to improve the sensitivity of the detection system, including hybridization chain reaction (HCR), invasion reaction, primer exchange reaction (PER), , enzyme cascade catalytic amplification, and catalytic hairpin assembly (CHA) reaction. , The emergence of these signal amplification methods has greatly improved the detection sensitivity of nucleic acid biosensors. In recent years, with the increasing maturity of signal amplification methods, dual signal amplification has gradually replaced single signal amplification platforms as a new trend to achieve ultrasensitive biosensing. , Zhao et al constructed a dual signal amplification platform using Exo III-mediated cyclic cleavage and classical rolling circle amplification reaction, which achieved sensitive detection of miRNA-21 in serum and cell lysate .…”

“…In general, increasing the sensitivity of a detection strategy often requires a combination of signal amplification techniques. Over the past few decades, a variety of signal amplification methods have been proposed to improve the sensitivity of the detection system, including hybridization chain reaction (HCR), 24 invasion reaction, primer exchange reaction (PER), 25,26 enzyme cascade catalytic amplification, 27 and catalytic hairpin assembly (CHA) reaction. 28,29 The emergence of these signal amplification methods has greatly improved the detection sensitivity of nucleic acid biosensors.…”