Electrochemical biosensor for rapid detection of fungal contamination in fuel systems

Radwan, Osman; Brothers, Michael; Coyle, Victoria; Chapleau, Molly E.; Chapleau, Richard R; Kim, Steve S.; Ruiz, Oscar N.

doi:10.1016/j.bios.2022.114374

Cited by 11 publications

(12 citation statements)

References 57 publications

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“…Briefly, the gold working electrode is composed of a self-assembled monolayer that includes an attached ferrocene, a fluorinated blocking group, and the Y. lipolyticacapture peptide (CHI) as the BRE that binds to an extracellular loop in the chitinase protein found upregulated in fuel-degrading fungi. , The response is compared to that of a nonspecific peptide for reference. 1 H ,1 H ,2 H ,2 H -Perfluorodecanethiol is attached as a blocking group for an omniphobic surface that prevents electrode surface fouling and fills vacant gold sites .…”

Section: Resultsmentioning

confidence: 99%

“…5,12 Given the market size, a number of yeast sensors have been developed for the food and beverage industry, 13−15 but there are significantly fewer sensors for biofuels. 7 Y. lipolytica interferes with long-term biofuel storage by contributing to the degradation of fuel sources. 16 Current quantification methods include optical instrumentation for counting of stained fluorescent cells, with useful concentrations from 10 to 10 6 CFU/mL for feedstock utilization.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In this work, we focus on a model system of Yarrowia lipolytica, a yeast commonly found in microbial growth in biofuels. ,, Fouling of fuels and in particular biofuels can both lead to significant spoilage and waste, while also costing significant man-hours to remedy . However, fuel tanks have diverse ecosystems, and thus, microbial contamination is complicated.…”

Section: Introductionmentioning

confidence: 99%

“…lipolytica, , can help monitor microbial growth and enable remediation at earlier time points. Similar sensor platforms and methods can then be leveraged in the food and beverage industry that uses yeast in their processes. , Given the market size, a number of yeast sensors have been developed for the food and beverage industry, − but there are significantly fewer sensors for biofuels Y.…”

Section: Introductionmentioning

confidence: 99%

“…6,8,9 Fouling of fuels and in particular biofuels can both lead to significant spoilage and waste, while also costing significant man-hours to remedy. 7 However, fuel tanks have diverse ecosystems, and thus, microbial contamination is complicated. As such, real-time monitoring of microbes, and in particular Y. lipolytica, 10,11 can help monitor microbial growth and enable remediation at earlier time points.…”

Section: ■ Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Advancing Ultrasensitive, Drift-Correcting Dual Floating Gate Organic Electrochemical Transistors for Yeast Sensing

Harris,

Brothers,

Coyle

et al. 2023

Chem. Mater.

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Impedance-based biosensors for microbes, such as yeast and bacteria, have been widely used as an alternative to optical sensing techniques. Yet, impedance-based techniques require complex electronics and data processing that are difficult to implement. Ultrasensitive, driftcorrecting electronic devices are needed for biological sensing in complex environments. Organic electrochemical transistors (OECTs) provide high signal amplification at low voltages, but most studies have focused extensively on semiconductor materials within the channels used as amplifiers. This study uses a number of interfacial electrochemical modifications to floating gate OECTs to create a universal platform for biological sensing focused on real-time monitoring of yeast concentrations as a proof-of-concept. Specifically, Y. lipolytica was selected as a model system, as it has been studied extensively for use within the oleochemical industry for production of biofuels and plastics. Real-time monitoring of the presence of these cells is needed for process control and storage in relatively complex environments. Using a combination of interfacial Faradaic mechanisms and reference and sample channel calibrations, a working range of 10 3 −10 6 CFU/mL is established, with a sensitivity of ∼6% per decade. One key advantage is that the output response using the dual floating gate OECT platform in this device architecture is significantly simpler than the output from electrochemical impedance spectroscopy and can be used as a base platform for future real-time field biosensors.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Advancing Ultrasensitive, Drift-Correcting Dual Floating Gate Organic Electrochemical Transistors for Yeast Sensing

Harris,

Brothers,

Coyle

et al. 2023

Chem. Mater.

Self Cite

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Synthetic biology for Monascus: From strain breeding to industrial production

Zhou,

Pan,

Xue

et al. 2024

Biotechnology Journal

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Traditional Chinese food therapies often motivate the development of modern medicines, and learning from them will bring bright prospects. Monascus, a conventional Chinese fungus with centuries of use in the food industry, produces various metabolites, including natural pigments, lipid‐lowering substances, and other bioactive ingredients. Recent Monascus studies focused on the metabolite biosynthesis mechanisms, strain modifications, and fermentation process optimizations, significantly advancing Monascus development on a lab scale. However, the advanced manufacture for Monascus is lacking, restricting its scale production. Here, the synthetic biology techniques and their challenges for engineering filamentous fungi were summarized, especially for Monascus. With further in‐depth discussions of automatic solid‐state fermentation manufacturing and prospects for combining synthetic biology and process intensification, the industrial scale production of Monascus will succeed with the help of Monascus improvement and intelligent fermentation control, promoting Monascus applications in food, cosmetic, agriculture, medicine, and environmental protection industries.

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An electrochemical proximity assay (ECPA) for antibody detection incorporating flexible spacers for improved performance

Kurian,

Mazumder,

Gurukandure

et al. 2024

Anal Bioanal Chem

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A clever approach for biosensing is to leverage the concept of the proximity effect, where analyte binding to probes can be coupled to a second, controlled binding event such as short DNA strands. This analyte-dependent effect has been exploited in various sensors with optical or electrochemical readouts. Electrochemical proximity assays (ECPA) are more amenable to miniaturization and adaptation to the point-of-care, yet ECPA has been generally targeted toward protein sensing with antibody-oligonucleotide probes. Antibodies themselves are also important as biomarkers, since they are produced in bodily fluids in response to various diseases or infections, often in low amounts. In this work, by using antigen-DNA conjugates, we targeted an ECPA method for antibody sensing and showed that the assay performance can be greatly enhanced using flexible spacers in the DNA conjugates. After adding flexible polyethylene glycol (PEG) spacers at two distinct positions, the spacers ultimately increased the antibody-dependent current by a factor of 4.0 without significant background increases, similar to our recent work using thermofluorimetric analysis (TFA). The optimized ECPA was applied to anti-digoxigenin antibody quantification at concentrations ranging over two orders of magnitude, from the limit of detection of 300 pM up to 50 nM. The assay was functional in 90% human serum, where increased ionic strength was used to counteract double-layer repulsion effects at the electrode. This flexible-probe ECPA methodology should be useful for sensing other antibodies in the future with high sensitivity, and the mechanism for signal improvement with probe flexibility may be applicable to other DNA-based electrochemical sensor platforms. Graphical Abstract

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Electrochemical biosensor for rapid detection of fungal contamination in fuel systems

Cited by 11 publications

References 57 publications

Advancing Ultrasensitive, Drift-Correcting Dual Floating Gate Organic Electrochemical Transistors for Yeast Sensing

Advancing Ultrasensitive, Drift-Correcting Dual Floating Gate Organic Electrochemical Transistors for Yeast Sensing

Synthetic biology for Monascus: From strain breeding to industrial production

An electrochemical proximity assay (ECPA) for antibody detection incorporating flexible spacers for improved performance

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