Electrochemical biotin determination based on a screen printed carbon electrode array and magnetic beads

Biscay, Julien; García, María Begoña González; Garcı́a, Agustı́n Costa

doi:10.1016/j.snb.2014.08.042

Cited by 25 publications

(16 citation statements)

References 44 publications

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“…Another competitive assay employing an electrochemical biosensor has been reported by Biscay et al [58]; this sensor incorporates the biological recognition element onto the magnetic beads and a magnet is used for direct assembly over the working carbon electrode. The sensitivity of this sensor was improved in a subsequent publication with the use of a flow injection analysis (FIA) system [59].…”

Section: Screen-printed Carbon Electrodes For Vitamin Analysismentioning

confidence: 99%

Recent Advances in the Fabrication and Application of Screen-Printed Electrochemical (Bio)Sensors Based on Carbon Materials for Biomedical, Agri-Food and Environmental Analyses

et al. 2016

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This review describes recent advances in the fabrication of electrochemical (bio)sensors based on screen-printing technology involving carbon materials and their application in biomedical, agri-food and environmental analyses. It will focus on the various strategies employed in the fabrication of screen-printed (bio)sensors, together with their performance characteristics; the application of these devices for the measurement of selected naturally occurring biomolecules, environmental pollutants and toxins will be discussed.

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Section: Screen-printed Carbon Electrodes For Vitamin Analysismentioning

confidence: 99%

Recent Advances in the Fabrication and Application of Screen-Printed Electrochemical (Bio)Sensors Based on Carbon Materials for Biomedical, Agri-Food and Environmental Analyses

et al. 2016

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“…Electrochemical DNA biosensor arrays, as a member of the electrochemical biosensor family, are widely used as powerful tools for the diagnosis of genetic and infectious diseases [ 4 , 5 ]. Many recent works have demonstrated the usage of screen-printed electrode arrays in order to carry out multiple measurements at the same time [ 5 , 6 , 7 , 8 , 9 ]. Recently, our group developed sensitive biosensors for the detection of protein, microRNA, and SNPs [ 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array

Erdem

Ekşin

2020

Biosensors

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A carbon nanofiber enriched 8-channel screen-printed electrochemical array was used for the impedimetric detection of SNP related to Factor V Leiden (FV Leiden) mutation, which is the most common inherited form of thrombophilia. FV Leiden mutation sensing was carried out in three steps: solution-phase nucleic acid hybridization between zip nucleic acid probe (Z-probe) and mutant type DNA target, followed by the immobilization of the hybrid on the working electrode area of array, and measurement by electrochemical impedance spectroscopy (EIS). TArzhe selectivity of the assay was tested against mutation-free DNA sequences and synthetic polymerase chain reaction (PCR) samples. The developed biosensor was a trustful assay for FV Leiden mutation diagnosis, which can effectively discriminate wild type and mutant type even in PCR samples.

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“…The disadvantages of this method are poor selectivity, tedious operation, and time‐consuming. In order to overcome these disadvantages, a variety of analytical methods including HPLC , TLC , CGE , flow injection analysis , electrochemical biosensor , CZE, and ELISA have been developed for biotin detection in the last 30 years. These methods show high sensitivity, being simpler and faster than the microbiological method.…”

Section: Introductionmentioning

confidence: 99%

A G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence assay for highly sensitive detection of biotin in flour

Zhao

et al. 2019

Electrophoresis

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Quantitative analysis of biotin in biological fluids, foods, and pharmaceutical is important for diagnosis and treatment of biotin‐related diseases and health maintenance. In this work, a novel G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence (CL) assay method was established for rapid and highly sensitive detection of biotin. This method is based on the specific binding between biotin and streptavidin, the catalytic CL characteristics of G‐quadruplex/hemin DNAzyme to the oxidation–reduction reaction of hydrogen peroxide with luminol, and the on‐line separation function of microchip electrophoresis. Under the optimal experimental conditions, on‐chip biotin analysis was achieved within 1 min. The CL intensity is linearly proportional to the concentration of biotin in the range of 13–630 nM with a detection limit of 6.4 nM. The proposed method has been applied for the detection of biotin in flour, biotin contents in three flour samples are found in the range of 199–223 ng/g with a mean value of 214 ng/g. The recoveries were in the range of 94–103%. With excellent sensitivity and good selectivity, the proposed method could be applied in a wide range of biological fluids, foods, and pharmaceutical analysis.

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Electrochemical biotin determination based on a screen printed carbon electrode array and magnetic beads

Cited by 25 publications

References 44 publications

Recent Advances in the Fabrication and Application of Screen-Printed Electrochemical (Bio)Sensors Based on Carbon Materials for Biomedical, Agri-Food and Environmental Analyses

Recent Advances in the Fabrication and Application of Screen-Printed Electrochemical (Bio)Sensors Based on Carbon Materials for Biomedical, Agri-Food and Environmental Analyses

Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array

A G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence assay for highly sensitive detection of biotin in flour

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