Electrochemical detection based on nanomaterials in CE and microfluidic systems

Sierra, Tania; Crevillén, Agustín G.; Escarpa, Alberto

doi:10.1002/elps.201800281

Cited by 35 publications

(20 citation statements)

References 57 publications

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“…Since MF devices can be coupled relatively easily to fluorescence detectors or fluorescence microscopes, the fluorescence of analytes is often used for their detection [ 84 ]. Recent developments in fluorescence detection in MF include fluorescence lifetime imaging, high-throughput single-molecule imaging, multicolor analysis, enhanced surface Raman spectroscopy, and surface plasmon resonance (SPR) detection [ 85 , 86 ].…”

Section: Fundamentals Of Microfluidicsmentioning

confidence: 99%

Microfluidics for Peptidomics, Proteomics, and Cell Analysis

et al. 2021

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Microfluidics is the advanced microtechnology of fluid manipulation in channels with at least one dimension in the range of 1–100 microns. Microfluidic technology offers a growing number of tools for manipulating small volumes of fluid to control chemical, biological, and physical processes relevant to separation, analysis, and detection. Currently, microfluidic devices play an important role in many biological, chemical, physical, biotechnological and engineering applications. There are numerous ways to fabricate the necessary microchannels and integrate them into microfluidic platforms. In peptidomics and proteomics, microfluidics is often used in combination with mass spectrometric (MS) analysis. This review provides an overview of using microfluidic systems for peptidomics, proteomics and cell analysis. The application of microfluidics in combination with MS detection and other novel techniques to answer clinical questions is also discussed in the context of disease diagnosis and therapy. Recent developments and applications of capillary and microchip (electro)separation methods in proteomic and peptidomic analysis are summarized. The state of the art of microchip platforms for cell sorting and single-cell analysis is also discussed. Advances in detection methods are reported, and new applications in proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices and determination of their physicochemical parameters are highlighted.

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Section: Fundamentals Of Microfluidicsmentioning

confidence: 99%

Microfluidics for Peptidomics, Proteomics, and Cell Analysis

et al. 2021

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“…Alongside the miniaturization of the sensing platform, other procedures such as extraction, separation, and washing steps should be miniaturized and automated to bring the laboratory-based food safety tests to the field. The electrochemical biosensor’s ease of miniaturization and integration makes them a suitable choice to be incorporated in either paper-based or chip- microfluidic devices, designed for automation and miniaturization of the sample extraction and handling processes [ 79 ]. Moreover, the potentiostat connectivity to the smartphone is an excellent way to make the electrochemical food safety analysis more user-friendly, minimizing the need for user training [ 80 ].…”

Section: Portable Electrochemical Food Analyzersmentioning

confidence: 99%

ASSURED Point-of-Need Food Safety Screening: A Critical Assessment of Portable Food Analyzers

Jafari

Guercetti

Geballa-Koukoula

et al. 2021

Foods

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Standard methods for chemical food safety testing in official laboratories rely largely on liquid or gas chromatography coupled with mass spectrometry. Although these methods are considered the gold standard for quantitative confirmatory analysis, they require sampling, transferring the samples to a central laboratory to be tested by highly trained personnel, and the use of expensive equipment. Therefore, there is an increasing demand for portable and handheld devices to provide rapid, efficient, and on-site screening of food contaminants. Recent technological advancements in the field include smartphone-based, microfluidic chip-based, and paper-based devices integrated with electrochemical and optical biosensing platforms. Furthermore, the potential application of portable mass spectrometers in food testing might bring the confirmatory analysis from the laboratory to the field in the future. Although such systems open new promising possibilities for portable food testing, few of these devices are commercially available. To understand why barriers remain, portable food analyzers reported in the literature over the last ten years were reviewed. To this end, the analytical performance of these devices and the extent they match the World Health Organization benchmark for diagnostic tests, i.e., the Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, and Deliverable to end-users (ASSURED) criteria, was evaluated critically. A five-star scoring system was used to assess their potential to be implemented as food safety testing systems. The main findings highlight the need for concentrated efforts towards combining the best features of different technologies, to bridge technological gaps and meet commercialization requirements.

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“…Electrochemical detection is an extremely selective and sensitive detection technique [116, 117]. Amperometric detection (AD) is the most commonly employed electrochemical detection technique.…”

Section: O‐phthaldialdehydementioning

confidence: 99%

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

Celá

Glatz

2020

Electrophoresis

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Amino acids are essential compounds for living organisms, and their determination in biological fluids is crucial for the clinical analysis and diagnosis of many diseases. However, the detection of most amino acids is hindered by the lack of a strong chromophore/fluorophore or electrochemically active group in their chemical structures. The highly sensitive determination of amino acids often requires derivatization. Capillary electrophoresis is a separation technique with excellent characteristics for the analysis of amino acids in biological fluids. Moreover, it offers the possibility of precapillary, on‐capillary, or postcapillary derivatization. Each derivatization approach has specific demands in terms of the chemistry involved in the derivatization, which is discussed in this review. The family of homocyclic o‐dicarboxaldehyde compounds, namely o‐phthalaldehyde, naphthalene‐2,3‐dicarboxaldehyde, and anthracene‐2,3‐dicarboxaldehyde, are powerful derivatization reagents for the determination of amino acids and related compounds. In the presence of suitable nucleophiles they react with the primary amino group to form both fluorescent and electroactive derivatives. Moreover, the reaction rate enables all of the derivatization approaches mentioned above. This review focuses on articles that deal with using these reagents for the derivatization of amino acids and related compounds for ultraviolet‐visible spectrometry, fluorescence, or electrochemical detection. Applications in capillary and microchip electrophoresis are summarized and discussed.

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Electrochemical detection based on nanomaterials in CE and microfluidic systems

Cited by 35 publications

References 57 publications

Microfluidics for Peptidomics, Proteomics, and Cell Analysis

Microfluidics for Peptidomics, Proteomics, and Cell Analysis

ASSURED Point-of-Need Food Safety Screening: A Critical Assessment of Portable Food Analyzers

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

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