Electrochemical detection of foodborne pathogen Aeromonas hydrophila by DNA hybridization biosensor

Tichoniuk, Mariusz; Gwiazdowska, Daniela; Ligaj, Marta; Filipiak, Marian

doi:10.1016/j.bios.2010.08.030

Cited by 53 publications

(32 citation statements)

References 24 publications

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“…The final applications include biomedical diagnostics [1][2][3], environmental [4] and food control [5,6], and safety and biohazard assessment [7,8]. In practice, unique characteristics distinguish (bio)electrochemical sensors from classical instrumental methods, for example spectroscopy, chromatography, and mass spectrometry; these include low cost, miniaturizability, ease of use, no interference from coloured or turbid samples, and applicability to raw samples for "in situ" and decentralized.…”

Section: Introductionmentioning

confidence: 99%

Bioelectroanalysis with nanoelectrode ensembles and arrays

Ongaro

Ugo

2012

Anal Bioanal Chem

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This review deals with recent advances in bioelectroanalytical applications of nanostructured electrodes, in particular nanoelectrode ensembles (NEEs) and arrays (NEAs). First, nanofabrication techniques, principles of function, and specific advantages and limits of NEEs and NEAs are critically discussed. In the second part, some recent examples of bioelectroanalytical applications are presented. These include use of nanoelectrode arrays and/or ensembles for direct electrochemical analysis of pharmacologically active organic compounds or redox proteins, and the development of functionalized nanoelectrode systems and their use as catalytic or affinity electrochemical biosensors.

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Section: Introductionmentioning

confidence: 99%

Bioelectroanalysis with nanoelectrode ensembles and arrays

Ongaro

Ugo

2012

Anal Bioanal Chem

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“…The ssDNA used in this work is a 20-mer oligonucleotides sequence that has been previously verified and reported to have high selectivity and specificity to A. hydrophila aerolysin gene [27]. A. hydrophila is a highly epidemic pathogen, mostly distributed in aquatic environments and food.…”

Section: A N U S C R I P Tmentioning

confidence: 99%

“…In all of them labeled ssDNA were used which has a difficult and expensive biochemical preparation [27,32,33].In this study, we used a new methodology for immobilization of ssDNA sequence on glassy carbon electrode. Reduced graphene oxide decorated cerium oxide nanocomposite/Polyaniline/glassy carbon electrode [CeO 2 NPs-RGO/PANI/GCE] was fabricated as a platform for immobilization of ssDNA sequence.…”

Section: A N U S C R I P Tmentioning

confidence: 99%

Detection of Aeromonas hydrophila DNA oligonucleotide sequence using a biosensor design based on Ceria nanoparticles decorated reduced graphene oxide and Fast Fourier transform square wave voltammetry

Jafari

Faridbod

Norouzi

et al. 2015

Analytica Chimica Acta

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“…Electrochemical DNA sensors have been used in food safety monitoring and the detection of pathogen DNA. Tichoniuk et al described an electrochemical DNA biosensor for the detection of Aeromonas hydrophila with a mixed self-assembled monolayer-modified electrode [29]. Ligaj et al presented two kinds of electrochemical DNA sensor for the detection of pathogenic bacteria [30].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

Sun

Wang

et al. 2015

J Solid State Electrochem

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In this paper, zirconia (ZrO 2 ) and graphene (GR) nanocomposite was electrodeposited on the surface of carbon ionic liquid electrode (CILE), which was used to construct an electrochemical DNA biosensor. GR was electroreduced from graphene oxide by potentiostatic method, and ZrO 2 nanoparticle was further electrodeposited on GR/CILE by cycling voltammetric scan in a ZrOCl 2 solution. The presence of GR on the electrode surface can provide a highly conductive interface with large surface area for the loading of ZrO 2 nanoparticles. Single-stranded DNA (ssDNA) probe sequences with phosphate group at the 5′ end could be easily immobilized on the surface of ZrO 2 /GR/CILE due to the strong affinity between ZrO 2 and phosphate groups. The ssDNA/ZrO 2 /GR/CILE was applied to hybridize with the target ssDNA sequence, and methylene blue (MB) was used as the electrochemical indicator. Due to the different binding models of MB with double-stranded DNA and ssDNA on the electrode surface, electrochemical response of MB was decreased after the hybridization reaction. Under the optimal conditions, the reduction peak current of MB was proportional to the concentration of Staphylococcus aureus nuc gene sequence in the range from 1.0×10 −13 to 1.0×10 −6 mol L −1 with the detection limit of 3.23 × 10 −14 mol L −1 (3σ). The electrochemical DNA sensor exhibited good selectivity to various mismatched ssDNA sequences, and the polymerase chain reaction amplification products of S. aureus nuc gene sequence were further detected with satisfactory results. Therefore, this electrochemical DNA sensor with ZrO 2 nanoparticles and GR nanosheet modified electrode could be used for the detection of specific ssDNA sequence in real biological samples.

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Electrochemical detection of foodborne pathogen Aeromonas hydrophila by DNA hybridization biosensor

Cited by 53 publications

References 24 publications

Bioelectroanalysis with nanoelectrode ensembles and arrays

Bioelectroanalysis with nanoelectrode ensembles and arrays

Detection of Aeromonas hydrophila DNA oligonucleotide sequence using a biosensor design based on Ceria nanoparticles decorated reduced graphene oxide and Fast Fourier transform square wave voltammetry

Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

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