Electrochemical Detection of Heparin Based on Its Interaction with Light Green

Abstract

The determination of heparin with linear sweep voltammetry has been developed in this paper based on its interaction with light green (LG). In pH 2.0 Britton -Robinson (B -R) buffer solution, LG has a sensitive linear sweep voltammetric reduction peak at À 0.41 V (vs. SCE) and heparin can react with LG to form an ion-association electroactive complex, which resulted in the decrease of the peak current of the reaction solution. Under the selected experimental conditions the decrease of peak current was directly proportional to the concentration of heparin in the range of 0.8 -20.0 mg/mL with the linear regression equation as Di '' p (nA) ¼ 79.12 C À 101.44 (n ¼ 14, g ¼ 0.996) and the detection limit as 0.28 mg/mL (3s). The effects of coexisting substances on the determination of heparin were investigated and the results showed that this method had good selectivity. The method was further applied to the determination of heparin content in heparin samples with satisfactory results and good recovery.

A reagentless enantioselective sensor for tryptophan enantiomers via nanohybrid matrices

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Linear sweep voltammetric studies on the supramolecular complex of alizarin red S with lysozyme and determination of lysozyme

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Multi-positively charged dendrimeric nanoparticles induced fluorescence quenching of graphene quantum dots for heparin and chondroitin sulfate detection

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