Electrochemical DNA biosensor for the detection of interaction between di[azino-di(5,6-azafluorene)-κ2-NN′]dichlormanganous and DNA

Liu, Nan; Li, Guang-Jiu; Li, Shufeng; Zhang, Shusheng

doi:10.1016/j.snb.2008.03.026

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…This result indicated that myriocin had the ability to bind to Fon DNA [22]. Changes in uorescence intensity can be used as evidence of the interaction between DNA and small molecules [23]. In the present study, EB uorescence intensity was decreased with the addition of myriocin compared to CK (Fig.…”

Section: Direct Damage To Dnasupporting

confidence: 59%

“…This nding indicated that the intercalated EB molecules on dsDNA could be replaced partially or fully by myriocin, which accounted for the decrease in uorescence intensity [24]. Thus, it could be concluded that the binding of myriocin with dsDNA occurred primarily by the intercalation mode, which accounted for the decrease in the migration rate of the myriocin-DNA bound complex [23,25].…”

Section: Direct Damage To Dnamentioning

confidence: 99%

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Comprehensive Transcriptomic and Proteomic Analyses Identify Intracellular Targets for Myriocin to Induce Fusarium Oxysporum F. Sp. Niveum Cell Death

Wang

et al. 2021

Preprint

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Background: Myriocin is a natural product with antifungal activity and is derived from Bacillus amyloliquefaciens LZN01. Our previous work demonstrated that myriocin can inhibit the growth of Fusarium oxysporum f. sp. niveum (Fon) by inducing membrane damage. In this study, the antifungal actions of myriocin against Fon were investigated with a focus on the effects of myriocin on intracellular molecules.Results: Analysis of DNA binding and fluorescence spectra demonstrated that myriocin can interact with dsDNA from Fon cells. The intracellular-targeted mechanism of action was also supported by transcriptomic and proteomic analyses; a total of 2238 common differentially expressed genes (DEGs) were identified. The DEGs were further verified by RT-qPCR. Most of the DEGs were assigned metabolism and genetic information processing functions and were enriched in ribosome biogenesis in eukaryotes pathway. The expression of some genes and proteins in ribosome biogenesis in eukaryotes pathway was affected by myriocin, primarily the genes controlled by the C6 zinc cluster transcription factor family and the NFYA transcription factor. Myriocin influenced the posttranscriptional processing of gene products by triggering the main RI (retained intron) events of novel alternative splicing; myriocin targeted key genes (FOXG_09470) or proteins (RIOK2) in ribosome biogenesis in eukaryotes pathway, resulting in disordered translation. Conclusions: In conclusion, myriocin was determined to exhibit activity against Fon by targeting intracellular molecules. The results of our study may help to elucidate the antifungal actions of myriocin against Fon.

show abstract

Section: Direct Damage To Dnasupporting

confidence: 59%

Section: Direct Damage To Dnamentioning

confidence: 99%

Comprehensive Transcriptomic and Proteomic Analyses Identify Intracellular Targets for Myriocin to Induce Fusarium Oxysporum F. Sp. Niveum Cell Death

Wang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…This result indicated that myriocin had the ability to bind to Fon DNA [ 22 ]. Changes in fluorescence intensity can be used as evidence of the interaction between DNA and small molecules [ 23 ]. In the present study, EB fluorescence intensity was decreased with the addition of myriocin compared to CK (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…This finding indicated that the intercalated EB molecules on dsDNA could be replaced partially or fully by myriocin, which accounted for the decrease in fluorescence intensity [ 24 ]. Thus, it could be concluded that the binding of myriocin with dsDNA occurred primarily by the intercalation mode, which accounted for the decrease in the migration rate of the myriocin-DNA bound complex [ 23 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive transcriptomic and proteomic analyses identify intracellular targets for myriocin to induce Fusarium oxysporum f. sp. niveum cell death

Wang

et al. 2021

Microb Cell Fact

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Background Myriocin is a natural product with antifungal activity and is derived from Bacillus amyloliquefaciens LZN01. Our previous work demonstrated that myriocin can inhibit the growth of Fusarium oxysporum f. sp. niveum (Fon) by inducing membrane damage. In this study, the antifungal actions of myriocin against Fon were investigated with a focus on the effects of myriocin on intracellular molecules. Results Analysis of DNA binding and fluorescence spectra demonstrated that myriocin can interact with dsDNA from Fon cells. The intracellular-targeted mechanism of action was also supported by transcriptomic and proteomic analyses; a total of 2238 common differentially expressed genes (DEGs) were identified. The DEGs were further verified by RT-qPCR. Most of the DEGs were assigned metabolism and genetic information processing functions and were enriched in ribosome biogenesis in eukaryotes pathway. The expression of some genes and proteins in ribosome biogenesis in eukaryotes pathway was affected by myriocin, primarily the genes controlled by the C6 zinc cluster transcription factor family and the NFYA transcription factor. Myriocin influenced the posttranscriptional processing of gene products by triggering the main RI (retained intron) events of novel alternative splicing; myriocin targeted key genes (FOXG_09470) or proteins (RIOK2) in ribosome biogenesis in eukaryotes pathway, resulting in disordered translation. Conclusions In conclusion, myriocin was determined to exhibit activity against Fon by targeting intracellular molecules. The results of our study may help to elucidate the antifungal actions of myriocin against Fon.

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Differential Pulse Voltammetric Detection of Hepatitis C Virus 1a Oligonucleotide Chain by a Label‐Free Electrochemical DNA Hybridization Biosensor Using Consensus Sequence of Hepatitis C Virus 1a Probe on the Pencil Graphite Electrode

2009

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The short sequence related to hepatitis C virus (HCV1) is detected by a label-free DNA hybridization biosensor. The sensor relies on the immobilization of a 20-mer oligonucleotide containing 2 guanine and 11 cytosine bases denoted PHCV1 as probe on the pencil graphite electrode (PGE). The hybridization event was monitored by differential pulse voltammetry (DPV) using the guanine signal. The selectivity of the biosensor was studied using some noncomplementary oligonucleotides. Diagnostic performance of the biosensor is described and the detection limit was found to be 6.5 nM.

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Electrochemical DNA biosensor for the detection of interaction between di[azino-di(5,6-azafluorene)-κ2-NN′]dichlormanganous and DNA

Cited by 10 publications

References 29 publications

Comprehensive Transcriptomic and Proteomic Analyses Identify Intracellular Targets for Myriocin to Induce Fusarium Oxysporum F. Sp. Niveum Cell Death

Comprehensive Transcriptomic and Proteomic Analyses Identify Intracellular Targets for Myriocin to Induce Fusarium Oxysporum F. Sp. Niveum Cell Death

Comprehensive transcriptomic and proteomic analyses identify intracellular targets for myriocin to induce Fusarium oxysporum f. sp. niveum cell death

Differential Pulse Voltammetric Detection of Hepatitis C Virus 1a Oligonucleotide Chain by a Label‐Free Electrochemical DNA Hybridization Biosensor Using Consensus Sequence of Hepatitis C Virus 1a Probe on the Pencil Graphite Electrode

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