Electrochemical dopamine detection: Comparing gold and carbon fiber microelectrodes using background subtracted fast scan cyclic voltammetry

Zachek, Matthew K.; Hermans, Andre; Wightman, R. Mark; McCarty, Gregory S.

doi:10.1016/j.jelechem.2007.11.007

Cited by 119 publications

(129 citation statements)

References 37 publications

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“…Different kinds of electrodes can be used to sensitively detect DA in the presence of AA and UA. Using FSCV, gold microelectrodes were found to be more sensitive to DA than carbon fiber microelectrodes [35]. In this work, the PPA film thickness can be controlled by the electropolymerization procedure.…”

Section: Interference Studymentioning

confidence: 96%

Selective Detection of Dopamine Using Glassy Carbon Electrode Modified by a Combined Electropolymerized Permselective Film of Polytyramine and Polypyrrole‐1‐propionic Acid

et al. 2009

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A sensitive and selective electrochemical method for the determination of dopamine using a combined electropolymerized permselective film of polytyramine and polypyrrole‐1‐propionic acid on a glassy carbon (GC) electrode was developed. The formation of a “layer‐by‐layer” film has allowed for selective detection of dopamine in the presence of 3,4‐dihydroxyphenylalanine (L‐DOPA), DOPAC, ascorbic acid, uric acid, epinephrine and norepinephrine. The modified electrodes exhibited a detection limit of 100 nM with linearity ranging from 5×10−6 to 5×10−5 M. No cleaning step was required during the course of repeated measurement.

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Section: Interference Studymentioning

confidence: 96%

Selective Detection of Dopamine Using Glassy Carbon Electrode Modified by a Combined Electropolymerized Permselective Film of Polytyramine and Polypyrrole‐1‐propionic Acid

et al. 2009

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“…Based on the square-scheme redox mechanism dominated by the adsorption/desorption processes established at classical carbon fiber disk electrodes, [17][18][19] one would expect the current peak intensities of the background corrected CVs, i p corr (v), to vary linearly with the scan rate over the range of scan rates considered in this study. However, the data reported in Figure 5a than that observed in the lower scan rate range (0.28 ± 0.01 nA.s.V −1 , Figure 5b and Table I).…”

Section: Discussionmentioning

confidence: 99%

“…Hence, it is safely concluded that the oxidation process exclusively involved mechanisms occurring in adsorbed phase as was established at carbon disk microelectrodes. 17,18 A second possible hypothesis which could have explained a drift of i p corr (v) away from linearity in adsorbed films consisted in assuming that at high scan rates the oxidation process was limited by electron-hopping [49][50][51][52][53][54][55] Table I). See text and Table I for the meaning of the other regression lines shown in (b-f).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the descending branches of CV did not followed the anticipated characteristic adsorptive behavior 39 as occurs at carbon disks microelectrodes. 17,18 These unexpected observations could not be explained through a competitive involvement of solution diffusion or electron-hopping in adsorbed films. Conversely, they could be qualitatively rationalized through considering that dopamine adsorbs onto electroactive and non-electroactive nanodomains interwoven over the electrode surface.…”

Section: G3058mentioning

confidence: 95%

“…Such high-aspect ratios are generally favored since they allow overcoming the micrometric-scale heterogeneity of synapses distributions in brain areas of interest. 23 However, in contrast with the near-homogeneity of electroactive properties of their cross-sections, 17,18 carbon fiber shafts are known…”

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confidence: 99%

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Surface Heterogeneities Matter in Fast Scan Cyclic Voltammetry Investigations of Catecholamines in Brain with Carbon Microelectrodes of High-Aspect Ratio: Dopamine Oxidation at Conical Carbon Microelectrodes

Oleinick

Álvarez‐Martos

Svir

et al. 2018

J. Electrochem. Soc.

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Fast Scan Cyclic Voltammetry (FSCV) at high aspect ratio carbon microelectrodes shows adequate high temporal and spatial resolution for in vivo analysis of catecholamines. Though the presence of their surface heterogeneities has been recognized since their earliest introduction for in vivo measurements in the brain, the kinetic consequences on the measurements have not been investigated and FSCV measurements are treated based on pre-and post-calibrations. We establish here that surface heterogeneities play a consequent dynamic role on the oxidation of dopamine taken as an example of catecholamines. Hence, the FSCV current peak intensities do not scale with the scan rate v or its square root. This is rationalized with a simple model involving a co-existence of at least two types of surface nanodomains with different electrochemical reactivities and different time responses. At low scan rates (<100 V s −1 ) dopamine molecules that initially adsorbed onto non-electroactive nanodomains have enough time to migrate toward highly electroactive ones so all molecules initially adsorbed on the whole electrode surface may be oxidized during one FSCV cycle. Current peak intensities then increase proportionally to the scan rate. However, above 100 V s −1 , dopamine migration between sites starts to be kinetically limited so that FSCV current peak intensities do not increase any more proportionally to the scan rate. Ultimately, i.e., above 1000 V s −1 , the dopamine exchange between sites is almost totally blocked so only dopamine molecules initially adsorbed on the electroactive surface nanodomains may be oxidized; the current peak intensities then increase again proportionally with the scan rate though with a smaller slope than that observed at small scan rates. Since carbon fibers with large aspect ratios are frequently used in brain investigations, this effect should be a concern when extracting quantitative results even when each carbon fiber response is properly pre-or post-calibrated using the exact CV waveform and scan rate used during the in vivo measurements. Release and modulation of neurotransmitters fluxes and concentrations are essential in complex organisms since they regulate many key functions by transferring information between cells such as neurons in the brain, neurons and muscle cells as well as through stimulating specific actions in endocrine-related systems. Albeit their unique and ubiquitous functions are well recognized today, the very existence and identification of neurotransmitters has long been a matter of debate up to the end of the second third of the past century, 1-3 and still needs to be better understood. This explains why characterizing fluxes and nature of neurotransmitters release is still stimulating a quest for analytical methods sufficiently accurate, specific and sensitive to investigate dynamic effluxes and flows of neurotransmitters in vivo (e.g., within a living brain) 4-6 or ex vivo (e.g., at the single endocrine cell level, 7-9 or intrasynaptically 10-12 ) with an adequate tempo...

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A Materials Roadmap to Functional Neural Interface Design

Wellman

Eles

Ludwig

et al. 2017

Adv Funct Materials

309

328

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Advancement in neurotechnologies for electrophysiology, neurochemical sensing, neuromodulation, and optogenetics are revolutionizing scientific understanding of the brain while enabling treatments, cures, and preventative measures for a variety of neurological disorders. The grand challenge in neural interface engineering is to seamlessly integrate the interface between neurobiology and engineered technology, to record from and modulate neurons over chronic timescales. However, the biological inflammatory response to implants, neural degeneration, and long-term material stability diminish the quality of interface overtime. Recent advances in functional materials have been aimed at engineering solutions for chronic neural interfaces. Yet, the development and deployment of neural interfaces designed from novel materials have introduced new challenges that have largely avoided being addressed. Many engineering efforts that solely focus on optimizing individual probe design parameters, such as softness or flexibility, downplay critical multi-dimensional interactions between different physical properties of the device that contribute to overall performance and biocompatibility. Moreover, the use of these new materials present substantial new difficulties that must be addressed before regulatory approval for use in human patients will be achievable. In this review, the interdependence of different electrode components are highlighted to demonstrate the current materials-based challenges facing the field of neural interface engineering.

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Electrochemical dopamine detection: Comparing gold and carbon fiber microelectrodes using background subtracted fast scan cyclic voltammetry

Cited by 119 publications

References 37 publications

Selective Detection of Dopamine Using Glassy Carbon Electrode Modified by a Combined Electropolymerized Permselective Film of Polytyramine and Polypyrrole‐1‐propionic Acid

Selective Detection of Dopamine Using Glassy Carbon Electrode Modified by a Combined Electropolymerized Permselective Film of Polytyramine and Polypyrrole‐1‐propionic Acid

Surface Heterogeneities Matter in Fast Scan Cyclic Voltammetry Investigations of Catecholamines in Brain with Carbon Microelectrodes of High-Aspect Ratio: Dopamine Oxidation at Conical Carbon Microelectrodes

A Materials Roadmap to Functional Neural Interface Design

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