Electrochemical enzymatic biosensor for tyramine based on polymeric matrix derived from 4-mercaptophenylacetic acid

Soares, Iara Pereira; Silva, Amanda Gonçalves da; Alves, Rafael da Fonseca; Corrêa, Ricardo Augusto Moreira de Souza; Ferreira, Lucas Franco; Franco, Diego Leoni

doi:10.1007/s10008-019-04204-w

Cited by 28 publications

(7 citation statements)

References 65 publications

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“…Here, analytes of interest are briefly discussed based on its corresponding enzyme–analyte system and the current output type, amperometric output. For readers with specific interests in impedance-based EEB outputs, we recommend these articles. − …”

Section: The Applications Of Bioelectrocatalysismentioning

confidence: 99%

Fundamentals, Applications, and Future Directions of Bioelectrocatalysis

et al. 2020

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Bioelectrocatalysis is an interdisciplinary research field combining biocatalysis and electrocatalysis via the utilization of materials derived from biological systems as catalysts to catalyze the redox reactions occurring at an electrode. Bioelectrocatalysis synergistically couples the merits of both biocatalysis and electrocatalysis. The advantages of biocatalysis include high activity, high selectivity, wide substrate scope, and mild reaction conditions. The advantages of electrocatalysis include the possible utilization of renewable electricity as an electron source and high energy conversion efficiency. These properties are integrated to achieve selective biosensing, efficient energy conversion, and the production of diverse products. This review seeks to systematically and comprehensively detail the fundamentals, analyze the existing problems, summarize the development status and applications, and look toward the future development directions of bioelectrocatalysis. First, the structure, function, and modification of bioelectrocatalysts are discussed. Second, the essentials of bioelectrocatalytic systems, including electron transfer mechanisms, electrode materials, and reaction medium, are described. Third, the application of bioelectrocatalysis in the fields of biosensors, fuel cells, solar cells, catalytic mechanism studies, and bioelectrosyntheses of high-value chemicals are systematically summarized. Finally, future developments and a perspective on bioelectrocatalysis are suggested.

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Section: The Applications Of Bioelectrocatalysismentioning

confidence: 99%

Fundamentals, Applications, and Future Directions of Bioelectrocatalysis

et al. 2020

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“…A recent paper [ 137 ] described the electropolymerization and characterization of a polymeric film of 4-mercaptophenylacetic acid to create covalent bonds between the carboxyl groups (left unchanged after electropolymerization by the sulfur atom) on the sensor surface and the tyrosinase subsequently attached by immersion of the electrode in the enzyme solution. The enzyme biosensor was used for the detection of tyramine.…”

Section: Tyrosinase Immobilization Strategiesmentioning

confidence: 99%

“…Without activation of the carboxyl groups in the formed polymer, morphological characterization shows the presence of electrostatic or weak Van der Waals-type interactions. Optimization of the parameters (pH, enzyme concentration) led to the formation of covalent bonds between enzyme and polymer and to a robust enzyme biosensor with high sensitivity and affinity for tyramine and efficient reproducibility in wine samples [ 137 ].…”

Section: Tyrosinase Immobilization Strategiesmentioning

confidence: 99%

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review

Bounegru

Apetrei

2023

Nanomaterials

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The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability to catalyze the oxidation of numerous types of phenolic compounds with antioxidant and neurotransmitter roles. This review critically examines the main tyrosinase immobilization techniques for the development of sensitive electrochemical biosensors. Immobilization strategies are mainly classified according to the degree of reversibility/irreversibility of enzyme binding to the support material. Each tyrosinase immobilization method has advantages and limitations, and its selection depends mainly on the type of support electrode, electrode-modifying nanomaterials, cross-linking agent or surfactants used. Tyrosinase immobilization by cross-linking is characterized by very frequent use with outstanding performance of the developed biosensors. Additionally, research in recent years has focused on new immobilization strategies involving cross-linking, such as cross-linked enzyme aggregates (CLEAs) and magnetic cross-linked enzyme aggregates (mCLEAs). Therefore, it can be considered that cross-linking immobilization is the most feasible and economical approach, also providing the possibility of selecting the reagents used and the order of the immobilization steps, which favor the enhancement of biosensor performance characteristics.

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“…To determine the Tra content of wines, a new functionalised polymer film was prepared by polymerisation of 4-mercaptophenylacetic acid (MPAA) and using CV. The tyrosinase enzyme was immobilised on carboxylic groups and DPV was used as the electrochemical measuring technique, resulting in an LOD and a limit of quantification (LOQ) of 3.16 μmol/L and 10.52 μmol/L, respectively [ 113 ].…”

Section: Monitoring Of Spoilage In Alcoholic and Non-alcoholic Beveragesmentioning

confidence: 99%

Application of Electrochemical Biosensors for Determination of Food Spoilage

2023

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Food security is significantly affected by the mass production of agricultural produce and goods, the growing number of imported foods, and new eating and consumption habits. These changed circumstances bring food safety issues arising from food spoilage to the fore, making food safety control essential. Simple and fast screening methods have been developed to detect pathogens and biomarkers indicating the freshness of food for safety. In addition to the traditional, sequential, chemical analytical and microbiological methods, fast, highly sensitive, automated methods suitable for serial tests have appeared. At the same time, biosensor research is also developing dynamically worldwide, both in terms of the analytes to be determined and the technical toolkit. Consequently, the rapid development of biosensors, including electrochemical-based biosensors, has led to significant advantages in the quantitative detection and screening of food contaminants. These techniques show great specificity for the biomarkers tested and provide adequate analytical accuracy even in complex food matrices. In our review article, we summarize, in separate chapters, the electrochemical biosensors developed for the most important food groups and the food safety issues they can ensure, with particular respect to meat and fish products, milk and dairy products, as well as alcoholic and non-alcoholic beverages.

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Electrochemical enzymatic biosensor for tyramine based on polymeric matrix derived from 4-mercaptophenylacetic acid

Cited by 28 publications

References 65 publications

Fundamentals, Applications, and Future Directions of Bioelectrocatalysis

Fundamentals, Applications, and Future Directions of Bioelectrocatalysis

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review

Application of Electrochemical Biosensors for Determination of Food Spoilage

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