Electrochemical genosensor assay using lyophilized gold nanoparticles/latex microsphere label for detection of Vibrio cholerae

Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification

“…The development of rapid, sensitive, and cost-effective methods is needed not only for the quick and appropriate treatment of patients infected with pathogenic Vibrio strains, but also for the prompt control and prevention of the diseases (Okada et al, 2010). To date, only few studies have been conducted on the detection of V. cholerae genes by the LAMP technique (He et al, 2009;Okada et al, 2010;Srisuk et al, 2010;Tourlousse et al, 2012;Liew et al, 2015;Zhang et al, 2015). In this study, an sssvLAMP method was successfully developed for the The magnesium is required for the LAMP reaction, however, the lower or higher concentration of Mg 2+ strongly influenced the LAMP reaction and its product.…”

“…To date, only few studies have been conducted to detect V. cholerae genes by the LAMP, i.g., the ctxA (Okada et al, 2010), hlyA (He et al, 2009), ompW (Srisuk et al, 2010), rtxA (Tourlousse et al, 2012), toxR (Zhang et al, 2015), and lolB (Liew et al, 2015). Development of a simple, rapid, specific and sensitive LAMP method for V. cholerae detection is very important for food safety control, particularly, as a highly efficient tool for large-scale screening of the bacterial contamination in water and aquatic products.…”

Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres

“…There are a few electrochemical methods reported for the detection of V. cholerae consisted of a series of complex steps. An electrochemical V. cholerae genosensor reported by Liew et al [ 24 ] used electrochemical adsorption method to immobilise the DNA probe on the carbon SPE. The lyophilised AuNPs-modified multilayer PSA particles with polyelectrolytes formed bioconjugate with avidin to function as the reporter label in the sandwich DNA hybridisation assay.…”

Lysozyme as a recognition element for monitoring of bacterial population

“…Biosensors have emerged as extremely useful tools for pathogenic detection. Various sensitive, reliable and rapid methods, such as electrochemical impedance spectroscopy [3,[6][7][8], surface plasma resonance [9,10], quartz crystal microbalance [3,11], have been reported for the determination and monitoring of microorganisms. The specific and direct detection of bacteria using monoclonal antibody and aptamer as recognition receptors immobilized covalently onto functionalized interface of biosensor or biochip are widespread developed.…”