Electrochemical genosensor based on disc and screen printed gold electrodes for detection of specific DNA and RNA sequences derived from Avian Influenza Virus H5N1

Malecka, Kamila; Stachyra, Anna; Góra-Sochacka, Anna; Sirko, Agnieszka; Zagórski-Ostoja, Włodzimierz; Radecka, Hanna; Radecki, Jerzy

doi:10.1016/j.snb.2015.10.044

Cited by 67 publications

(24 citation statements)

References 42 publications

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“…The antigen-antibody complex formation as well as hybridization process suppresses the accessibility of redox marker toward the electrode surface. This phenomenon, which is the base of analytical signal generation by ion-channel mimetic mode, was observed using the Osteryoung square-wave voltammetry OSWV or electrochemical impedance spectroscopy EIS in the presence of [Fe CN ] 3−/4− as an electroactive marker [ 14].…”

Section: Introductionmentioning

confidence: 93%

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

Radecka¹,

Radecki²

2016

Steps Forwards in Diagnosing and Controlling Influenza

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Abstract"vian inluenza viruses "IVs could cause severe diseases and, as the consequence, serious economic losses as well as a risk for potential transmission to humans. Therefore, the detection of viruses and their fragments or speciic DN" sequences becomes an important approach in molecular diagnosis. Here, we present electrochemical genosensors devoted for detection of inluenza virus H5N1 gene sequence. We focus our atention on ion-channel mechanism, E-DN" sensors, and genosensors based on redox-active layer. The novel dual DN" electrochemical sensor with "signal-of" and "signal-on" architecture for simultaneous detection of two diferent sequences of DN" derived from avian inluenza virus type H5N1 by means of one electrode is presented. Immunosensors are also adequate analytical devices for detection of pathogens since antibodies are natural receptors responsible for binding of antigens. Thus, the binding selectivity and eiciency are naturally high. The immunosensors presented could be divided into two main groups: ion-channel mimetic and based on redox-active monolayer.

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Section: Introductionmentioning

confidence: 93%

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

Radecka¹,

Radecki²

2016

Steps Forwards in Diagnosing and Controlling Influenza

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“…In the recent years, many examples have been proposed for the electrochemical detection of DNA and RNA sequences using disposable electrodes [64][65][66][67][68][69][70][71]. Among these, many are those based on different signal amplification procedures that allow to achieve very low detection limits [72][73][74][75][76][77][78][79][80].…”

Section: Dna-based Electrochemical Disposable Sensorsmentioning

confidence: 99%

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Arduini

Micheli

Moscone

et al. 2016

TrAC Trends in Analytical Chemistry

336

166

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A B S T R A C TThis review addresses recent advances in the development of screen-printed electrode based biosensors modified with different nanomaterials such as carbon nanotubes, graphene, metallic nanoparticles as gold, silver and magnetic nanoparticles, and mediator nanoparticles (Prussian Blue, Cobalt Phthalocyanine, etc.), coupled with biological recognition elements such as enzymes, antibodies, DNA and aptamers to obtain probes with improved analytical features. Examples of clinical applications are illustrated, together with examples of paper-based electrochemical devices, of multiple detections using arrays of screen printed electrodes, and of the most recent developments in the field of wearable biosensors. Also the use of smartphones as final detectors is briefly depicted.

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“…180 bp) and RNA transcripts (ca. 280 nt) containing regions complementary to the probe (Malecka et al, 2012, Malecka et al, 2015, Malecka et al, 2016Grabowska et al, 2013, Grabowska et al, 2014a, Grabowska et al, 2014bKurzątkowska et al, 2015;Kaur et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive electrochemical genosensor for direct detection of specific RNA sequences derived from avian influenza viruses present in biological samples

et al. 2019

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An electrochemical genosensor based on an epoxy-phenanthroline–Fe(III)–NH2-ssDNA layer for the detection of RNA derived from Avian Influenza is presented. The biosensor preparation consists of: (I) modification of gold electrodes with aminoethanethiol, (II) modification of the self-assembled monolayer of aminoethanethiol with 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline using “click” chemistry, (III) a first step of complexation of Fe(III) by 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline, (IV) a second step of complexation of Fe(III) by 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline, (V) immobilization of the single stranded amino-DNA probe via “click” chemistry between epoxy and amino groups. The interactions between the ssDNA probe and RNA targets were explored with Osteryoung Square Wave Voltammetry. The genosensor showed a remarkable detection limit of 3 copies/µL (5 aM) for RNA extracted from A/swan/Poland/305/06 (H5N1) containing a fully complementary sequence. A linear dynamic range for this sequence was observed from 3.0×103 to 3.0×105 [copies/µl]. RNA extracted from A/mallard/Poland/446/09 (H7N7), containing a non-complementary sequence, generated a much weaker response. Moreover, the developed genosensor allows to distinguish RNA present in biological samples having 2, 3 and 4 mismatches. This biosensing approach can become a potential alternative tool for detecting RNA samples in biomedical research and early clinical diagnosis of avian influenza viruses.

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Electrochemical genosensor based on disc and screen printed gold electrodes for detection of specific DNA and RNA sequences derived from Avian Influenza Virus H5N1

Cited by 67 publications

References 42 publications

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Ultrasensitive electrochemical genosensor for direct detection of specific RNA sequences derived from avian influenza viruses present in biological samples

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