Electrochemical Immuno-Vascular Solid State Point of Care Testing (POCT)

Abstract

Abstract:In this work, it is proposed a POCT and innovative method of immunoassay for the detection of C-reactive protein and IgG, using Amperometry coupled to solid state kit, connected to a micro-flow system with comparable sensitivity to a high sensitivity CRP ELISA (hsCRP) and IgG ELISA, with 1-3 min turnaround time to result. Samples of CRP (0 to 250 ng·mL -1 ) andIgG or diluted spiked human serum are injected through a solid state polymeric kit, micro-flow sensor channels of a SWCT SPE nano-modified biosensor. Preparing two kits immuno-assays, in the same micro-column, built on oxirane groups of polymeric bead surface, with biological support to CRP and IgG biomarkers recognition, in a real time scheme, at the end of analyte injection the initial rate of change in current intensity I/A was proportional to CRP respectively IgG concentration, with low detection limit (LOD) of 0.1 ng·mL -1 . It was revealed that CRP/IgG concentrations in serum that might be expected in both normal and pathological conditions can be detected in a real-time-efficient, multi-immunoassay with solid state detection kit technology with determined CRP/IgG concentrations in close agreement with those determined using a commercially available high sensitivity ELISA.