Electrochemical immunosensor based on Ag+-dependent CTAB-AuNPs for ultrasensitive detection of sulfamethazine

Yang, Mingyue; Wu, Xiangyang; Hu, Xialin; Wang, Kun; Zhang, Can; Gyimah, Eric; Yakubu, Salome; Zhang, Zhen

doi:10.1016/j.bios.2019.111643

Cited by 29 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Until 2010, not many electrochemical immunosensors based on CNHs were reported. During the last ten years, they have been employed both as electrode materials [76][77][78][79] and as electrocatalytic tracers/nanocarriers [40,80,81].…”

Section: Carbon-based Nanomaterials (Nms)mentioning

confidence: 99%

Nanostructure-Based Electrochemical Immunosensors as Diagnostic Tools

et al. 2021

View full text Add to dashboard Cite

Electrochemical immunosensors are affinity-based biosensors characterized by several useful features such as specificity, miniaturizability, low cost and simplicity, making them very interesting for many applications in several scientific fields. One of the significant issues in the design of electrochemical immunosensors is to increase the system’s sensitivity. Different strategies have been developed, one of the most common is the use of nanostructured materials as electrode materials, nanocarriers, electroactive or electrocatalytic nanotracers because of their abilities in signal amplification and biocompatibility. In this review, we will consider some of the most used nanostructures employed in the development of electrochemical immunosensors (e.g., metallic nanoparticles, graphene, carbon nanotubes) and many other still uncommon nanomaterials. Furthermore, their diagnostic applications in the last decade will be discussed, referring to two relevant issues of present-day: the detection of tumor markers and viruses.

show abstract

Section: Carbon-based Nanomaterials (Nms)mentioning

confidence: 99%

Nanostructure-Based Electrochemical Immunosensors as Diagnostic Tools

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Interestingly, from the 26 studies presented in Table 2 , only two resorted to SWCNT for (bio)sensor enhancement [ 125 , 126 ]. Since both MWCNT and SWCNT have similar electrocatalytic properties, the later nanostructure is lesser used probably due to lower synthesis yield and higher production costs [ 132 ].…”

Section: Nanostructured Carbon-based (Bio)sensors For Pharmaceuticmentioning

confidence: 99%

“…The biosensor was ultimately validated in spiked tap water with recoveries of about 100%. Whereas, the SWCNT-based biosensor developed by Yang et al [ 125 ] relied in a competitive immunoassay intended for environmental screening of the antibiotic drug sulfamethazine. In order to amplify the electrochemical signal, a first layer of SWCNT (in the form of nanohorns) dispersed in chitosan (Chit) was deposited on a GCE surface followed by a layer of cetyltrimethylammonium bromide-capped gold nanoparticles (CTAB-AuNPs).…”

Section: Nanostructured Carbon-based (Bio)sensors For Pharmaceuticmentioning

confidence: 99%

“…With the increase of the drug concentration, less AgNPs were available in the biosensor surface leading therefore to a proportional decrease in the H 2 O 2 catalytic response. The immunosensor achieved a low value of LOD corresponding to 2.4 × 10 −4 µmol L −1 and it was finally validated in spiked river, lake, and pond waters achieving satisfactory recoveries (79–118%), though the intra-assay variation was up to 10% for this biosensor [ 125 ]. Other CNT-based sensors were developed for different sulfonamide drugs [ 123 , 124 ].…”

Section: Nanostructured Carbon-based (Bio)sensors For Pharmaceuticmentioning

confidence: 99%

See 1 more Smart Citation

Application of Nanostructured Carbon-Based Electrochemical (Bio)Sensors for Screening of Emerging Pharmaceutical Pollutants in Waters and Aquatic Species: A Review

et al. 2020

View full text Add to dashboard Cite

Pharmaceuticals, as a contaminant of emergent concern, are being released uncontrollably into the environment potentially causing hazardous effects to aquatic ecosystems and consequently to human health. In the absence of well-established monitoring programs, one can only imagine the full extent of this problem and so there is an urgent need for the development of extremely sensitive, portable, and low-cost devices to perform analysis. Carbon-based nanomaterials are the most used nanostructures in (bio)sensors construction attributed to their facile and well-characterized production methods, commercial availability, reduced cost, high chemical stability, and low toxicity. However, most importantly, their relatively good conductivity enabling appropriate electron transfer rates—as well as their high surface area yielding attachment and extraordinary loading capacity for biomolecules—have been relevant and desirable features, justifying the key role that they have been playing, and will continue to play, in electrochemical (bio)sensor development. The present review outlines the contribution of carbon nanomaterials (carbon nanotubes, graphene, fullerene, carbon nanofibers, carbon black, carbon nanopowder, biochar nanoparticles, and graphite oxide), used alone or combined with other (nano)materials, to the field of environmental (bio)sensing, and more specifically, to pharmaceutical pollutants analysis in waters and aquatic species. The main trends of this field of research are also addressed.

show abstract

“…11,12 Although these methods are standardized, accurate and reliable, their wide applications are limited by high costs and complex sample preparations. 13 In addition, antibody-dependent methods including enzyme-linked immunosorbent assay (ELISA), 14 chemiluminescence, 15 time-resolved immunoassay, 16,17 lateral flow immunoassay 18 and electrochemical immunosensor 19 also face different challenges such as poor stabilities, high costs, and difficulties in modifying antibody structures. 20 Aptamers are single-stranded DNA or RNA that bind specifically to target molecules, and they are selected by an in vitro process called systematic evolution of ligands by exponential enrichment (SELEX).…”

Section: Introductionmentioning

confidence: 99%

An Ultrasensitive Label-Free Fluorescent Aptasensor Platform for Detection of Sulfamethazine

Wang¹,

Yan²,

Kou³

et al. 2021

IJN

View full text Add to dashboard Cite

Purpose: Sulfamethazine (SMZ) exposed in the environment can enter the human body through the food chain and pose a serious threat to human health. Therefore, it is important to develop a rapid and sensitive method for detecting SMZ in environmental samples. In order to fastly and quantitatively detect SMZ in environmental samples, we developed a label-free fluorescent aptasensor based on specific aptamer (SMZ1S) and fluorescence resonance energy transfer (FRET) between gold nanoparticles (AuNPs) and rhodamine B (RhoB). Methods: In the absence of SMZ, SMZ1S was adsorbed on the surface of AuNPs, which led to dispersion of the AuNPs in high concentration saline solution, thus effectively quenching the fluorescence of RhoB. With the increase of the SMZ concentration, the specific binding of SMZ1S and SMZ led to the aggregation of AuNPs in the presence of NaCl, which reduced the quenching of RhoB fluorescence and increased the fluorescence intensity. The sensitivity and linearity curve of the label-free fluorescent aptasensor were determined with different concentrations of sulfamethazine standard solutions. The specificity of this fluorescent aptasensor was determined by replacing sulfamethazine with different antibiotics. In addition, the actual water and soil samples were spiked and recovered. Results: Under optimized conditions, the proposed fluorescent aptasensor demonstrated a good linear detection of SMZ in binding buffer from 1.25 ng mL −1 to 40 ng mL −1 and the limit of detection was 0.82 ng mL −1 . The spiked recoveries for SMZ were 94.4% to 108.8% with a relative standard deviation of 1.8-10.3% in water and soil samples, respectively. Conclusion:The label-free fluorescent aptasensor investigated in the current study is a promising tool to detect and quantify SMZ in water and soil samples.

show abstract

Electrochemical immunosensor based on Ag+-dependent CTAB-AuNPs for ultrasensitive detection of sulfamethazine

Cited by 29 publications

References 34 publications

Nanostructure-Based Electrochemical Immunosensors as Diagnostic Tools

Nanostructure-Based Electrochemical Immunosensors as Diagnostic Tools

Application of Nanostructured Carbon-Based Electrochemical (Bio)Sensors for Screening of Emerging Pharmaceutical Pollutants in Waters and Aquatic Species: A Review

An Ultrasensitive Label-Free Fluorescent Aptasensor Platform for Detection of Sulfamethazine

Contact Info

Product

Resources

About