Electrochemical Immunosensor Based on Antibody-Nanoparticle Hybrid for Specific Detection of the Dengue Virus NS1 Biomarker

Darwish, Nadiya Taha; Alrawi, Aoday Hashim; Sekaran, Shamala Devi; Alias, Yatimah; Khor, Sook Mei

doi:10.1149/2.0471603jes

Cited by 38 publications

(22 citation statements)

References 46 publications

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“…5a) with a sensitivity of~22% decade −1 , a RSD (%) of 7 ± 4 ( Fig. 5a) and a LoD of 0.5 ng mL −1 , thus approximately sixty-fold lower than those obtained by EIS-ES and ten-fold lower than an immunosensor based on EIS reported in the literature by others (Darwish et al, 2016). In addition, the repeatability of the NS1 biosensors was assessed using five interday and five intra-day replicate measurements of a 50 ng mL −1 standard solution in neat serum wherein the coefficients of variation obtained were 7% and 8%, for ECS, while values of 6% and 3% were achieved by EIS.…”

Section: Ns1 Protein Detection In Serummentioning

confidence: 72%

The capacitive sensing of NS1 Flavivirus biomarker

Cecchetto

Fernandes

Lopes

et al. 2017

Biosensors and Bioelectronics

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NS1 is a biomarker for different Flavivirus diseases such as dengue (DENV), zika (ZIKV) and chikungunya (CHIKV) and was herein selectively quantified by electrochemical capacitive sensing (an impedance-derived capacitance methodology wherein the redox probe is contained in the receptive layer) mainly aiming dengue diagnosis in phosphate buffer saline and blood serum environments (up to the neat level). The capacitive sensing was compared to traditional concurrent impedimetric approach (in which the redox probe is added in the biological solution) and other transient methods stated in the literature regarding figures of merit such as limit of detection, linear range, relative standard deviation and affinity constant. Capacitive and impedimetric assays showed equivalent results for linear range, repeatability, sensitivity and constant of affinity. Nonetheless capacitive assays presented better reproducibility with a relative standard deviation (RSD) of 3±1 and 7±4 (all in percentage) in PBS and serum, respectively, meanwhile for impedimetric assays the RSD values were 9±5 in PBS and 12±6 in serum. Thus, by using capacitive assays, an improvement on the analytical performance was observed with the limit of detection about sixty-fold lower in neat serum (∼0.5ngmL for capacitive over ∼30ngmL for impedimetric assays) compared to traditional electrochemistry methods in general hence demonstrating the superior detection sensitivity for NS1 protein. Accordingly, redox tagged capacitive assays are suitable for the development of multiplex point-of-care neglected diseases sensing applications.

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Section: Ns1 Protein Detection In Serummentioning

confidence: 72%

The capacitive sensing of NS1 Flavivirus biomarker

Cecchetto

Fernandes

Lopes

et al. 2017

Biosensors and Bioelectronics

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“…Regarding viral infection determination [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ], electrochemical biosensing approaches have been focused mainly on the detection of: the whole virus such as human enterovirus 71 [ 55 ]; specific viral proteins (p24 from HIV [ 46 , 47 , 48 , 49 ] and NS1 from Dengue [ 54 ]); autoantibodies against specific viral antigens (pseudorabies virus [ 50 ], HIV-1 and HIV-2 [ 51 , 56 ] and hemagglutinin, HA, from avian influenza virus H5N1 [ 52 , 53 ]) and characteristic DNA sequences (Dengue virus serotype 3, DENV-3, [ 57 ] and high-risk human papillomavirus, hrHPV, strains HPV-16 and HPV-18 [ 58 ]). …”

Section: Electrochemical Affinity Biosensing Of Infectious Pathogementioning

confidence: 99%

Molecular Biosensors for Electrochemical Detection of Infectious Pathogens in Liquid Biopsies: Current Trends and Challenges

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

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Rapid and reliable diagnosis of infectious diseases caused by pathogens, and timely initiation of appropriate treatment are critical determinants to promote optimal clinical outcomes and general public health. Conventional in vitro diagnostics for infectious diseases are time-consuming and require centralized laboratories, experienced personnel and bulky equipment. Recent advances in electrochemical affinity biosensors have demonstrated to surpass conventional standards in regards to time, simplicity, accuracy and cost in this field. The tremendous potential offered by electrochemical affinity biosensors to detect on-site infectious pathogens at clinically relevant levels in scarcely treated body fluids is clearly stated in this review. The development and application of selected examples using different specific receptors, assay formats and electrochemical approaches focusing on the determination of specific circulating biomarkers of different molecular (genetic, regulatory and functional) levels associated with bacterial and viral pathogens are critically discussed. Existing challenges still to be addressed and future directions in this rapidly advancing and highly interesting field are also briefly pointed out.

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“…A sensitive, specific, and label‐free electrochemical immunosensor was developed for early and reliable diagnosis of dengue virus infection though the determination of the unstructured protein NS1 secreted during the early phase of the disease with a concentration of up to 50 μg mL −1 . The immunosensing scaffold employed ITO electrodes modified by in situ generated aryl diazonium cations derived zwitterionic antifouling molecules, AuNPs and monoclonal antibodies.…”

Section: Tropical Diseasesmentioning

confidence: 99%

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

2017

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Rapid and reliable diagnosis of viral infections and tropical diseases and timely initiation of appropriate treatment are essential for their successful clinical management. This Review summarizes some basic concepts regarding the main viral infection and tropical diseases to which society is facing today and the conventional methods available for their detection. The tremendous potential offered by electrochemical affinity biosensors to address this important challenge, meeting the required demands for viral infections and tropical diseases diagnosis, is clearly stated by discussing challenges, opportunities, implementation, and application of selected examples focused on the determination of specific biomarkers of different molecular (genetic, regulatory, and functional) levels. The highlighted approaches demonstrate the use of a plethora of specific receptors and assay formats and a great diversity of attractive electrochemical approaches in this rapidly advancing and highly interesting field.

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Electrochemical Immunosensor Based on Antibody-Nanoparticle Hybrid for Specific Detection of the Dengue Virus NS1 Biomarker

Cited by 38 publications

References 46 publications

The capacitive sensing of NS1 Flavivirus biomarker

The capacitive sensing of NS1 Flavivirus biomarker

Molecular Biosensors for Electrochemical Detection of Infectious Pathogens in Liquid Biopsies: Current Trends and Challenges

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

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