Electrochemical immunosensor based on chitosan/conductive carbon black composite modified disposable ITO electrode: An analytical platform for p53 detection

Aydın, Elif Burcu; Aydın, Muhammet; Sezgintürk, Mustafa Kemal

doi:10.1016/j.bios.2018.09.008

Cited by 87 publications

(38 citation statements)

References 41 publications

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“…UV irradiation produces abundant hydroxyl groups on the MAO surface, which can be reacted with the ethoxy groups of APTES [37]. The terminal amine group can then be reacted with the aldehyde groups of glutaraldehyde, which is commonly applied for crosslinking with biomolecules [49,50]. Then, chitosan can be covalently conjugated to the MAO Ti surface via the APTES-GA layer, which guaranteed the stability of the self-assembled multilayers on the Ti surface.…”

Section: Discussionmentioning

confidence: 99%

Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

Liu

et al. 2020

J Nanobiotechnol

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Background The biofunctionalization of titanium implants for high osteogenic ability is a promising approach for the development of advanced implants to promote osseointegration, especially in compromised bone conditions. In this study, polyelectrolyte multilayers (PEMs) were fabricated using the layer-by-layer approach with a chitosan-miRNA (CS-miRNA) complex and sodium hyaluronate (HA) as the positively and negatively charged polyelectrolytes on microarc-oxidized (MAO) Ti surfaces via silane-glutaraldehyde coupling. Methods Dynamic contact angle and scanning electron microscopy measurements were conducted to monitor the layer accumulation. RiboGreen was used to quantify the miRNA loading and release profile in phosphate-buffered saline. The in vitro transfection efficiency and the cytotoxicity were investigated after seeding mesenchymal stem cells (MSCs) on the CS-antimiR-138/HA PEM-functionalized microporous Ti surface. The in vitro osteogenic differentiation of the MSCs and the in vivo osseointegration were also evaluated. Results The surface wettability alternately changed during the formation of PEMs. The CS-miRNA nanoparticles were distributed evenly across the MAO surface. The miRNA loading increased with increasing bilayer number. More importantly, a sustained miRNA release was obtained over a timeframe of approximately 2 weeks. In vitro transfection revealed that the CS-antimiR-138 nanoparticles were taken up efficiently by the cells and caused significant knockdown of miR-138 without showing significant cytotoxicity. The CS-antimiR-138/HA PEM surface enhanced the osteogenic differentiation of MSCs in terms of enhanced alkaline phosphatase, collagen production and extracellular matrix mineralization. Substantially enhanced in vivo osseointegration was observed in the rat model. Conclusions The findings demonstrated that the novel CS-antimiR-138/HA PEM-functionalized microporous Ti implant exhibited sustained release of CS-antimiR-138, and notably enhanced the in vitro osteogenic differentiation of MSCs and in vivo osseointegration. This novel miRNA-functionalized Ti implant may be used in the clinical setting to allow for more effective and robust osseointegration.

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Section: Discussionmentioning

confidence: 99%

Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

Liu

et al. 2020

J Nanobiotechnol

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“…UV irradiation can produce abundant hydroxyl groups on the MAO surface, which could perfectly combine with the ethoxy groups of APTES [38]. The terminal amine group can then be utilized for reaction with the aldehyde groups of glutaraldehyde, which is commonly applied to cross-link with biomolecules [47,48]. Then, chitosan can be conjugated with a stable covalent bonding onto the MAO Ti surface via the APTES-GA layer, which guarantees stability of the whole self-assembled multilayers on the Ti surface.…”

Section: Discussionmentioning

confidence: 99%

Chitosan-miRNA Functionalized Microporous Titanium Oxide Surface via A Layer-by-layer Approach with a Sustained Release Profile for Enhanced Osteogenic Activity

Liu

et al. 2020

Preprint

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Background: Biofunctionalization of titanium implants for high osteogenic ability holds the hope for advanced implant of promoted osseointegration, especially in the compromised bone condition. In this study, using chitosan-miRNA (CS-miRNA) complex and sodium hyaluronate (HA) as the positively and negatively charged polyelectrolyte, the polyelectrolyte multilayers (PEMs) were fabricated using the layer-by-layer approach on the microarc oxidized (MAO) Ti surface via silane glutaraldehyde coupling. Methods: Dynamic contact angle and scanning electron microscopy were firstly analyzed to monitor the layer accumulation. RiboGreen was used to quantify the miRNA loading and release profile in phosphate-buffered saline. In vitro transfection efficiency and cytotoxicity were investigated after mesenchymal stem cells (MSCs) being seeded on the CS-antimiR-138/HA PEM functionalized microporous Ti surface. The in vitro osteogenic differentiation of MSCs and in vivo osseointegration were also inspected. Results: The surface wettability alternately changed during the formation of PEMs. The CS-miRNA nanoparticles distributed evenly along the MAO surface. The miRNA loading amount increased with the bilayer number increasing. More importantly, a sustained miRNA release of over approximately 2 week was obtained. In vitro transfection revealed that the CS-antimiR-138 nanoparticles were taken up efficiently by the cells and caused significant knockdown of miR-138 without showing significant cytotoxicity. The CS-antimiR-138/HA PEM surface enhanced osteogenic differentiation of MSCs on it in terms of enhanced alkaline phosphatase, collagen product and extracellular matrix mineralization. In the rat model, it led to dramatically enhanced in vivo osseointegration. Conclusions: All these findings demonstrate that novel CS-antimiR-138/HA PEM functionalized microporous Ti implant exhibits sustained release of CS-antimiR-138, and obviously enhances the in vitro osteogenic differentiation of MSCs and dramatically enhanced in vivo osseointegration. This novel miRNA functionalized Ti implant may be used in clinic to allow more effective and robust osseointegration.

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“…This requirement of a high dilution factor might arise from the existence of other intricate proteins (such as serum albumin) in the human serum that could interfere with the response. 35,36 Thereupon, three different cTnT concentrations (1, 10 and 100 pg mL -1 cTnT-Ag) were formulated by artificially spiking the cTnT stock solution into the diluted human serum. The observed analytical data were tabulated in the Table 1.…”

Section: Real Samples Analysesmentioning

confidence: 99%

A Label-free Cardiac Troponin T Electrochemiluminescence Immunosensor Enhanced by Graphene Nanoplatelets

et al. 2019

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In this study, a direct and label-free immunosensor was designed and constructed by modifying the screen-printed electrode with graphene nanoplatelets (GNPs) for the detection of the cardiac troponin T (cTnT). Firstly, GNPs were drop-casted onto carbon working electrode. Monoclonal cTnT antibodies were then immobilized on the GNPs via physical adsorption; finally, BSA was introduced to block non-specific binding sites. The detection of cTnT was performed using an electrochemiluminescence (ECL) technique with tris(bipyridine)ruthenium(II) chloride ([Ru(bpy)3]Cl2) used as a luminophore and TPrA (tripropylamine) as a co-reactant. The ECL intensity was demonstrated to be directly proportional to the cTnT concentration where a linear range from 100 pg mL-1 to 5 fg mL-1 of the cTnT detection was established. An extremely low limit of detection was achieved to be 0.05 fg mL-1 with an outstanding specificity. Additionally, this immunosensor showed excellent percentage recovery for real samples analyses in artificially spiked human serum.

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Electrochemical immunosensor based on chitosan/conductive carbon black composite modified disposable ITO electrode: An analytical platform for p53 detection

Cited by 87 publications

References 41 publications

Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

Chitosan-miRNA Functionalized Microporous Titanium Oxide Surface via A Layer-by-layer Approach with a Sustained Release Profile for Enhanced Osteogenic Activity

A Label-free Cardiac Troponin T Electrochemiluminescence Immunosensor Enhanced by Graphene Nanoplatelets

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