Electrochemical immunosensor based on chitosan-gold nanoparticle/carbon nanotube as a ﻿platform and lactate oxidase as a ﻿label for detection of CA125 oncomarker﻿

Pakchin, Parvin Samadi; Ghanbari, Hossein; Saber, Reza; Omidi, Yadollah

doi:10.1016/j.bios.2018.09.016

Cited by 158 publications

(38 citation statements)

References 45 publications

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“…26 Among all nanomaterials, gold nanoparticles (AuNPs) are an attractive candidate for delivery of various anticancer agents due to their individual properties including biocompatibility, easy conjugation to biomolecules such as antibody and enzyme, and the unique optical properties, which increase the binding rate to amine and thiol groups, allowing surface modification. [27][28][29][30] In our previous study, it was shown that a novel nanobiomedicine composed of AuNPs conjugated with Bovine RNase A (RnaGNPs) was able to overcome the 150°C under N for 5 hours with an azeotropic distillation apparatus. Toluene was removed under reduced pressure.…”

Section: Synthesis Of Peg Ligands Sh-peg-coohmentioning

confidence: 99%

PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells

et al. 2019

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Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy. Bovine pancreatic ribonuclease A (RNase A) represents a new class of cytotoxic and non-mutagenic enzymes, and has gained more attention as a potential anticancer modality; however, the cytosolic ribonuclease inhibitors (RIs) restrict the clinical application of this enzyme. Nowadays, nanotechnology-based diagnostic and therapeutic systems have provided potential solutions for cancer treatments. Methods: In this study, the gold nanoparticles (AuNPs) were synthesized, stabilized by polyethylene glycol (PEG), functionalized, and covalently conjugated with RNase A. The physicochemical properties of engineered nanobiomedicine (AuNPs-PEG-RNase A) were characterized by scanning electron microscope (SEM), dynamic light scattering (DLS), and UV-vis spectrum. Then, its biological impacts including cell viability, apoptosis, and ROS production were evaluated in the SW-480 cells. Results: The engineered nanobiomedicine, AuNPs-PEG-RNase A, was found to effectively induce apoptosis in SW-480 cells and result in a significant reduction in cancer cell viability. Besides, the maximum production of ROS was obtained after the treatment of cells with an IC50 dose of AuNPs-PEG-RNase A. Conclusion: Based on the efficient ROS-responsiveness and the anticancer activity of RNase A of the engineered nanomedicine, this nanoscaled biologics may be considered as a potential candidate for the treatment of CRC.

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Section: Synthesis Of Peg Ligands Sh-peg-coohmentioning

confidence: 99%

PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells

et al. 2019

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“…The prepared immunosensor also displayed satisfactory performance when quantifying the NT-proBNP in practical plasma samples, which revealed that the ABEI/COOH-MWCNTs/chitosan/GNDs nanomaterial was a superior electrochemical sensing platform. Recently, Omidi et al [51] constructed a simple electrochemical immunosensor for the determination of carcinoma antigen 125 (CA125) based on chitosan-gold nanoparticle/multiwall carbon nanotube/graphene oxide (CS-AuNP/MWCNT/GO) platform. The lactate oxidase is applied as the single-enzyme label in electrochemical immunosensor for the first time.…”

Section: Nanomaterials Based Electrochemical Immunosensormentioning

confidence: 99%

“…Schematic illustration of the immunosensor preparation process and possible mechanism of electrochemical reaction. Reproduced with permission from [51]. …”

Section: Figurementioning

confidence: 99%

Nanomaterials-Based Electrochemical Immunosensors

et al. 2019

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With the development of nanomaterials and sensor technology, nanomaterials-based electrochemical immunosensors have been widely employed in various fields. Nanomaterials for electrode modification are emerging one after another in order to improve the performance of electrochemical immunosensors. When compared with traditional detection methods, electrochemical immunosensors have the advantages of simplicity, real-time analysis, high sensitivity, miniaturization, rapid detection time, and low cost. Here, we summarize recent developments in electrochemical immunosensors based on nanomaterials, including carbon nanomaterials, metal nanomaterials, and quantum dots. Additionally, we discuss research challenges and future prospects for this field of study.

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“…Biosensors mediated optical measurements have usually proven to be reliable, faster, compact, and user-friendly than those applying electrochemical and conventional optical bio-sensing systems [ 1 , 2 ]. Sensitive bio-sensing technologies such as surface plasmon resonance (SPR), electrochemical biosensors and recently inverse opal photonic crystals (IOPCs) biosensors, have been employed as diagnostic devices for the detection of biomarkers [ [3] , [4] , [5] , [6] , [7] , [8] ]. Photonic crystals (PCs) are dielectric and nanostructured materials with optical sensing properties and wide range applications in the field of optoelectronics and photonics [ [9] , [10] , [11] ].…”

Section: Introductionmentioning

confidence: 99%

Photonic crystal based biosensors: Emerging inverse opals for biomarker detection

Fathi

Rashidi

Pakchin

et al. 2021

Talanta

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Photonic crystal (PC)-based inverse opal (IO) arrays are one of the substrates for label-free sensing mechanism. IO-based materials with their advanced and ordered three-dimensional microporous structures have recently found attractive optical sensor and biological applications in the detection of biomolecules like proteins, DNA, viruses, etc. The unique optical and structural properties of IO materials can simplify the improvements in non-destructive optical study capabilities for point of care testing (POCT) used within a wide variety of biosensor research. In this review, which is an interdisciplinary investigation among nanotechnology, biology, chemistry and medical sciences, the recent fabrication methodologies and the main challenges regarding the application of (inverse opals) IOs in terms of their bio-sensing capability are summarized.

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Electrochemical immunosensor based on chitosan-gold nanoparticle/carbon nanotube as a platform and lactate oxidase as a label for detection of CA125 oncomarker

Cited by 158 publications

References 45 publications

PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells

PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells

Nanomaterials-Based Electrochemical Immunosensors

Photonic crystal based biosensors: Emerging inverse opals for biomarker detection

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Electrochemical immunosensor based on chitosan-gold nanoparticle/carbon nanotube as a ﻿platform and lactate oxidase as a ﻿label for detection of CA125 oncomarker﻿

Cited by 158 publications

References 45 publications

PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells

PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells

Nanomaterials-Based Electrochemical Immunosensors

Photonic crystal based biosensors: Emerging inverse opals for biomarker detection

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Product

Resources

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Electrochemical immunosensor based on chitosan-gold nanoparticle/carbon nanotube as a platform and lactate oxidase as a label for detection of CA125 oncomarker