Electrochemical Immunosensor for Carcinoembryonic Antigen Based on Antigen Immobilization in Gold Nanoparticles Modified Chitosan Membrane

Jun, Lin; Qu, Wei; Zhang, Shusheng

doi:10.2116/analsci.23.1059

Cited by 23 publications

(9 citation statements)

References 51 publications

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“…Under optimized conditions the proposed immunosensor shows a linear range for CEA concentrations from 2.0–20 ng mL −1 with a detection limit of 1.0 ng mL −1 . The proposed electrode is cost effective, has good stability, is biocompatible, nontoxic and can be mass-produced industrially [ 95 ].…”

Section: Application Of Nanostructured Materials To Immunosensorsmentioning

confidence: 99%

Prospects of Nanotechnology in Clinical Immunodiagnostics

Ansari

Alhoshan

AlSalhi

et al. 2010

Sensors

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Nanostructured materials are promising compounds that offer new opportunities as sensing platforms for the detection of biomolecules. Having micrometer-scale length and nanometer-scale diameters, nanomaterials can be manipulated with current nanofabrication methods, as well as self-assembly techniques, to fabricate nanoscale bio-sensing devices. Nanostructured materials possess extraordinary physical, mechanical, electrical, thermal and multifunctional properties. Such unique properties advocate their use as biomimetic membranes to immobilize and modify biomolecules on the surface of nanoparticles. Alignment, uniform dispersion, selective growth and diameter control are general parameters which play critical roles in the successful integration of nanostructures for the fabrication of bioelectronic sensing devices. In this review, we focus on different types and aspects of nanomaterials, including their synthesis, properties, conjugation with biomolecules and their application in the construction of immunosensing devices. Some key results from each cited article are summarized by relating the concept and mechanism behind each sensor, experimental conditions and the behavior of the sensor under different conditions, etc. The variety of nanomaterial-based bioelectronic devices exhibiting novel functions proves the unique properties of nanomaterials in such sensing devices, which will surely continue to expand in the future. Such nanomaterial based devices are expected to have a major impact in clinical immunodiagnostics, environmental monitoring, security surveillance and for ensuring food safety.

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Section: Application Of Nanostructured Materials To Immunosensorsmentioning

confidence: 99%

Prospects of Nanotechnology in Clinical Immunodiagnostics

Ansari

Alhoshan

AlSalhi

et al. 2010

Sensors

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show abstract

“…In their follow‐up study, they reported another CEA immunosensor based on AuNPs/Nano‐CaCO 3 /Prussian‐blue modified glassy carbon electrodes with a detection limit of 0.1 ng/ml . Moreover, Lin et al fabricated a disposable CEA immunosenser with a detection limit of 1 ng/ml by immobilizing CEA antibody on AuNPs‐chitosan membrane modified indium‐tin oxide (ITO) electrodes . ITO electrodes make such sensors significantly cheaper than sensors based on glassy‐carbon electrodes.…”

Section: Nanodevices For In Vitro Applicationsmentioning

confidence: 99%

Perspectives and potential applications of nanomedicine in breast and prostate cancer

Yang

Solomon

Achilefu

2010

Medicinal Research Reviews

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Nanomedicine is a branch of nanotechnology that includes the development of nanostructures and nanoanalytical systems for various medical applications. Among these applications, utilization of nanotechnology in oncology has captivated the attention of many research endeavors in recent years. The rapid development of nano-oncology raises new possibilities in cancer diagnosis and treatment. It also holds great promise for realization of point-of-care, theranostics, and personalized medicine. In this article, we review advances in nano-oncology, with an emphasis on breast and prostate cancer because these organs are amenable to the translation of nanomedicine from small animals to humans. As new drugs are developed, the incorporation of nanotechnology approaches into medicinal research becomes critical. Diverse aspects of nano-oncology are discussed, including nanocarriers, targeting strategies, nanodevices, as well as nanomedical diagnostics, therapeutics, and safety. The review concludes by identifying some limitations and future perspectives of nano-oncology in breast and prostate cancer management.

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“…Thus, several electrochemical methods have been reported for hCG [84], CEA [85] and CA 125 [86] determination in serum using HRP-antibody conjugated as tracer. These assays are based on the immobilization of the antigen in AuNPs coated on the surface of a glassy carbon [84,86] or an indium-tin oxide [85] electrode, using trimethoxysilane [84], chitosan [85] or cellulose acetate [86] to stabilize the AuNPs in the corresponding electrode. After the interfacial competitive immunoreaction, the tracer showed enzymatic activity for the oxidation of o-phenylenediamine by hydrogen peroxide.…”

Section: Competitive Assaysmentioning

confidence: 99%