Electrochemical immunosensor for human chorionic gonadotropin based on horseradish peroxidase–functionalized Prussian blue–carbon nanotubes/gold nanocomposites as labels for signal amplification

Yang, Hongchuan; Yuan, Ruo; Chai, Yaqin; Su, Huilan; Zhuo, Ying; Jiang, Wen; Song, Zhongju

doi:10.1016/j.electacta.2010.12.003

Cited by 41 publications

(16 citation statements)

References 35 publications

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“…Chitosan was used for preventing PB leakage and to adsorb AuNPs which were further used to immobilize HRP-Ab 2 . A high sensitivity was attained by DPV detection of H 2 O 2 [41]. The electrochemical properties of Ni-MWCNTs composites could be improved by doping with cobalt and further addition of boron and phosphate.…”

Section: Carbon Nanotubes Used As Carriers For Signal Elements In Elementioning

confidence: 91%

“…Chitosan was used for preventing PB leakage and to adsorb AuNPs which were further used to immobilize HRP-Ab2. A high sensitivity was attained by DPV detection of H2O2 [41]. The Prussian Blue (PB) has been widely used as a mediator in electrochemical biosensors because of its high electronic catalytic activity and stability among other properties [46].…”

Section: Carbon Nanotubes Used As Carriers For Signal Elements In Elementioning

confidence: 99%

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Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Growing demand for developing ultrasensitive electrochemical bioassays has led to the design of numerous signal amplification strategies. In this context, carbon-based nanomaterials have been demonstrated to be excellent tags for greatly amplifying the transduction of recognition events and simplifying the protocols used in electrochemical biosensing. This relevant role is due to the carbon-nanomaterials' large surface area, excellent biological compatibility and ease functionalization and, in some cases, intrinsic electrochemistry. These carbon-based nanomaterials involve well-known carbon nanotubes (CNTs) and graphene as well as the more recent use of other carbon nanoforms. This paper briefly discusses the advantages of using carbon nanostructures and their hybrid nanocomposites for amplification through tagging in electrochemical biosensing platforms and provides an updated overview of some selected examples making use of labels involving carbon nanomaterials, acting both as carriers for signal elements and as electrochemical tracers, applied to the electrochemical biosensing of relevant (bio)markers.

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Section: Carbon Nanotubes Used As Carriers For Signal Elements In Elementioning

confidence: 91%

“…Chitosan was used for preventing PB leakage and to adsorb AuNPs which were further used to immobilize HRP-Ab2. A high sensitivity was attained by DPV detection of H2O2 [41]. The Prussian Blue (PB) has been widely used as a mediator in electrochemical biosensors because of its high electronic catalytic activity and stability among other properties [46].…”

Section: Carbon Nanotubes Used As Carriers For Signal Elements In Elementioning

confidence: 99%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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“…3(A), the immobilized PB only exhibits weak catalytic response toward H 2 O 2 . These phenomena suggest that the linkaged HRP of the HRP/HRP-Ab 2 /GO/Tcd B/Ab 1 / GA/CS/PB/MWCNTs/GCE was efficiently in the catalytic reduction of H 2 O 2 with PB, that was used as a mediator to produce electrochemical signals and also to synergize HRP for the reduction of H 2 O 2 for amplification of the signals (Yang et al, 2011;Zhong et al, 2010). And it also suggests that the electrocatalytic reactions involved the participation of the HRP conjugated to immobilized Tcd B antibody.…”

Section: Signal Amplification Immunoassay With Hrp/hrp-ab 2 /Go-cooh mentioning

confidence: 96%

Simple approach for ultrasensitive electrochemical immunoassay of Clostridium difficile toxin B detection

Fang

Chen

Huang

et al. 2014

Biosensors and Bioelectronics

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“…However, the methods mentioned above have some limitations in terms of sensitivity, selectivity, stability and operation. Recently, electrochemical immunosensor has received considerable attention because of the significant advantages [5][6][7]. Compared with the sandwich-type immunosensor, label-free electrochemical immunosensor is much simpler and more cost effective, because there is no need of secondary antibody.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Immunosensor for Ultrasensitive Detection of Human Chorionic Gonadotropin Based on Pd@SBA‐15

Zhang

Shi

et al. 2012

Electroanalysis

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A simple and sensitive electrochemical immunosensor was conducted for the determination of human chorionic gonadotropin (hCG) with Pd@SBA-15. Thionine (TH) was selected as an electron transfer mediator, and modified onto the electrode together with functionalized graphene nanomaterial (HSO 3 À GS) through electrostatic adsorption. Then Pd@SBA-15 was immobilized onto the as-prepared film for biomolecules anchoring. Pd@SBA-15 composites not only retain the good biocompatibility of the SBA-15, but also exhibit an excellent catalytic activity of Pd and low Pd leaching. hCG antibody was immobilized onto the composite film for the detection of hCG. hCG can be determined in the range of 0.01-16.00 ng/mL and the detection limit is 8.60 pg/mL. The method has been applied to the analysis of hCG in human serum samples with satisfactory results.

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Electrochemical immunosensor for human chorionic gonadotropin based on horseradish peroxidase–functionalized Prussian blue–carbon nanotubes/gold nanocomposites as labels for signal amplification

Cited by 41 publications

References 35 publications

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Simple approach for ultrasensitive electrochemical immunoassay of Clostridium difficile toxin B detection

Electrochemical Immunosensor for Ultrasensitive Detection of Human Chorionic Gonadotropin Based on Pd@SBA‐15

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