Electrochemical immunosensor for ochratoxin A detection based on Au octahedron plasmonic colloidosomes

Zhang, Tong; Xing, Bin; Han, Qingzhi; Ye, Lei; Wu, Dan; Ren, Xiang; Wei, Qin

doi:10.1016/j.aca.2018.05.035

Cited by 52 publications

(15 citation statements)

References 48 publications

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“…Giving the strong capacity of BSA to capture OTA, as demonstrated in this work, BSA is not an adequate blocking reagent for methodologies that intend to detect OTA, although it is recurrently used for this purpose (e.g. Zhang et al, 2018). The drawback of using BSA as a blocking agent is the possible enhancement or suppression of assays response due to the interference of OTA that binds to the protein, conducting to misleading results.…”

Section: Validation Of Bsa-agarose Spe For Ota Determination In Winementioning

confidence: 97%

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

et al. 2019

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A B S T R A C TAnalytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R 2 = 0.9999) in the OTA concentration range studied (0.05 to 3.0 μg L −1 ), with recovery rates above 98%. It also showed low detection (0.017 μg L −1 ) and quantification (0.051 μg L −1 ) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results.

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Section: Validation Of Bsa-agarose Spe For Ota Determination In Winementioning

confidence: 97%

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

et al. 2019

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“…The European Commission established some regulatory limits to control OTA levels and, for example, the maximum tolerated level for raw cereal grains is 5 ppb [83]. Among the numerous methods for OTA biosensing, it is worth mentioning the preparation of an electrochemical immunosensor involving octahedral plasmonic colloidosomes (AuOctPCs) as substrates for the immobilization of specific OTA antibodies and as labels for signal amplification [84]. Octahedral gold nanoparticles (OctAuNPs), obtained by the reduction of HAuCl 4 in the presence of PDDA (poly(diallyldimethylammonium) and ethylene glycol, were used to immobilize the capture antibody onto the electrode surface.…”

Section: Toxinsmentioning

confidence: 99%

“…Ab 1 and Ab 2 capture and detector OTA antibodies, respectively. Reproduced from[84], with permission.…”

mentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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“…Here, the use of hierarchical structures, complex morphologies of nanomaterials have shown relatively superior performance compared with their traditional counterparts. Particular, in terms of electro-catalytic activity, the complex morphologies are known to exhibit superior performances owing to the mishmash of free surface energy, the charge density concentrated at the corners of the complex morphology, and the larger total surface area [7][8][9][10][11]. On the other hand, the use of heterojunction is also a promising approach to acquire a high throughput signal.…”

Section: Introductionmentioning

confidence: 99%

“…Rapid and selective detection of PCT from human serum samples would be beneficial for an early diagnosis, thereby decreasing the overall response time for the treatment. Contrary to the conventional approaches such as radioimmunoassay [4], colorimetric immunoassay [5], and enzyme-linked immunosorbent assay [6], the electrochemical approach designed for the detection of such molecules offers the advantage of high sensitivity, rapid detection time, and the capability to be transformed into a miniaturized device for field portability [7,8]. As the concentration of PCT in normal blood is less than 0.1 ng mL −1 [3], a highly sensitive electrochemical system is required for its accurate detection.…”

Section: Introductionmentioning

confidence: 99%

In Situ Growth of CuWO4 Nanospheres over Graphene Oxide for Photoelectrochemical (PEC) Immunosensing of Clinical Biomarker

Abbas

Soomro

Kalwar

et al. 2019

Sensors

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Procalcitonin (PCT) protein has recently been identified as a clinical marker for bacterial infections based on its better sepsis sensitivity. Thus, an increased level of PCT could be linked with disease diagnosis and therapeutics. In this study, we describe the construction of the photoelectrochemical (PEC) PCT immunosensing platform based on it situ grown photo-active CuWO4 nanospheres over reduced graphene oxide layers (CuWO4@rGO). The in situ growth strategy enabled the formation of small nanospheres (diameter of 200 nm), primarily composed of tiny self-assembled CuWO4 nanoparticles (2–5 nm). The synergic coupling of CuWO4 with rGO layers constructed an excellent photo-active heterojunction for photoelectrochemical (PEC) sensing. The platform was then considered for electrocatalytic (EC) mechanism-based detection of PCT, where inhibition of the photocatalytic oxidation signal of ascorbic acid (AA), subsequent to the antibody–antigen interaction, was recorded as the primary signal response. This inhibition detection approach enabled sensitive detection of PCT in a concentration range of 10 pg·mL−1 to 50 ng.mL−1 with signal sensitivity achievable up to 0.15 pg·mL−1. The proposed PEC hybrid (CuWO4@rGO) could further be engineered to detect other clinically important species.

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Electrochemical immunosensor for ochratoxin A detection based on Au octahedron plasmonic colloidosomes

Cited by 52 publications

References 48 publications

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

In Situ Growth of CuWO4 Nanospheres over Graphene Oxide for Photoelectrochemical (PEC) Immunosensing of Clinical Biomarker

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