Electrochemical impedance spectroscopy, another arrow in the arsenal to study the biodegradability of two-dimensional materials

Didonè, Livia; Shin, Yunseok; Silvestri, Alessandro; Prato, Maurizio; Park, Sungjin; Bianco, Alberto

doi:10.1039/d3nr04502a

Cited by 1 publication

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References 28 publications

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“…The objective of this study was to validate EIS as a versatile, cost-effective, and user-friendly alternative with broad applicability across diverse experimental conditions. EIS is recognized for its sensitivity and precision in biological applications and offers new insights into enzyme kinetic analysis [ 19 , 20 , 21 , 22 , 23 , 24 ]. Moreover, EIS provides comparable speed and accuracy to traditional fluorescence spectroscopy, while also reducing operational costs and increasing user-friendliness.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy for the Sensing of the Kinetic Parameters of Engineered Enzymes

Dusíková,

Baranová,

Krahulec

et al. 2024

Sensors

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The study presents a promising approach to enzymatic kinetics using Electrochemical Impedance Spectroscopy (EIS) to assess fundamental parameters of modified enteropeptidases. Traditional methods for determining these parameters, while effective, often lack versatility and convenience, especially under varying environmental conditions. The use of EIS provides a novel approach that overcomes these limitations. The enteropeptidase underwent genetic modification through the introduction of single amino acid modifications to assess their effect on enzyme kinetics. However, according to the one-sample t-test results, the difference between the engineered enzymes and hEKL was not statistically significant by conventional criteria. The kinetic parameters were analyzed using fluorescence spectroscopy and EIS, which was found to be an effective tool for the real-time measurement of enzyme kinetics. The results obtained through EIS were not significantly different from those obtained through traditional fluorescence spectroscopy methods (p value >> 0.05). The study validates the use of EIS for measuring enzyme kinetics and provides insight into the effects of specific amino acid changes on enteropeptidase function. These findings have potential applications in biotechnology and biochemical research, suggesting a new method for rapidly assessing enzymatic activity.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy for the Sensing of the Kinetic Parameters of Engineered Enzymes

Dusíková,

Baranová,

Krahulec

et al. 2024

Sensors

View full text Add to dashboard Cite

show abstract

Electrochemical impedance spectroscopy, another arrow in the arsenal to study the biodegradability of two-dimensional materials

Abstract: Carbon nitrides represent an emerging class of two-dimensional materials exploited for energy storage, for catalysis, in composites, and in biomedicine. Their biodegradability has been studied under the conditions of the photo-Fenton reaction.

Cited by 1 publication

References 28 publications

Electrochemical Impedance Spectroscopy for the Sensing of the Kinetic Parameters of Engineered Enzymes

Electrochemical Impedance Spectroscopy for the Sensing of the Kinetic Parameters of Engineered Enzymes

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