Electrochemical impedance spectroscopy for black lipid membranes fused with channel protein supported on solid-state nanopore

Khan, Muhammad S.; Dosoky, Noura S.; Berdiev, Bakhrom K.; Williams, John D.

doi:10.1007/s00249-016-1156-8

Cited by 38 publications

(49 citation statements)

References 52 publications

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“…The thin base layer structures fabricated inside a silicon microstructure had cavity depths of ~493 ± 6 μm was fabricated using 30% KOH at 55 °C for four days followed by room temperature treatment for 4 h. A detailed description for the production of this silicon-based micron cavity was previously discussed in [ 28 ]. Figure 1 shows an image on the left from scanning electron microscopy (SEM) of a typical Si cavity fabricated in this work.…”

Section: Resultsmentioning

confidence: 99%

Lipid Bilayer Membrane in a Silicon Based Micron Sized Cavity Accessed by Atomic Force Microscopy and Electrochemical Impedance Spectroscopy

et al. 2017

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Supported lipid bilayers (SLBs) are widely used in biophysical research to probe the functionality of biological membranes and to provide diagnoses in high throughput drug screening. Formation of SLBs at below phase transition temperature (Tm) has applications in nano-medicine research where low temperature profiles are required. Herein, we report the successful production of SLBs at above—as well as below—the Tm of the lipids in an anisotropically etched, silicon-based micro-cavity. The Si-based cavity walls exhibit controlled temperature which assist in the quick and stable formation of lipid bilayer membranes. Fusion of large unilamellar vesicles was monitored in real time in an aqueous environment inside the Si cavity using atomic force microscopy (AFM), and the lateral organization of the lipid molecules was characterized until the formation of the SLBs. The stability of SLBs produced was also characterized by recording the electrical resistance and the capacitance using electrochemical impedance spectroscopy (EIS). Analysis was done in the frequency regime of 10−2–105 Hz at a signal voltage of 100 mV and giga-ohm sealed impedance was obtained continuously over four days. Finally, the cantilever tip in AFM was utilized to estimate the bilayer thickness and to calculate the rupture force at the interface of the tip and the SLB. We anticipate that a silicon-based, micron-sized cavity has the potential to produce highly-stable SLBs below their Tm. The membranes inside the Si cavity could last for several days and allow robust characterization using AFM or EIS. This could be an excellent platform for nanomedicine experiments that require low operating temperatures.

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Section: Resultsmentioning

confidence: 99%

Lipid Bilayer Membrane in a Silicon Based Micron Sized Cavity Accessed by Atomic Force Microscopy and Electrochemical Impedance Spectroscopy

et al. 2017

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“…Many devices of this type exist and are popular on the medical market; the examples are vein filters and catheters used for prevention of pulmonary embolism and drug delivery [48]. Here, we assume that the top of the detector is covered with a layer of channelrhodopsin molecules, which are arranged in a squared matrix and located on a lipid membrane, using so called black lipid membrane technique [49,50]. Above the channelrhodopsin layer ( Fig.…”

Section: Example Of Medical Data Gathering Systemmentioning

confidence: 99%

Signal generation and storage in FRET-based nanocommunications

Kmiecik

Wójcik

Kułakowski

et al. 2019

Nano Communication Networks

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The paper is concerned with Fӧrster Resonance Energy Transfer (FRET) considered as a mechanism for communication between nanodevices. Two solved issues are reported in the paper, namely: signal generation and signal storage in FRET-based nanonetworks. First, luciferase molecules as FRET transmitters which are able to generate FRET signals themselves, taking energy from chemical reactions without any external light exposure, are proposed. Second, channelrhodopsins as FRET receivers, as they can convert FRET signals into voltage, are suggested. Further, medical in-body systems where both molecule types might be successfully applied, are discussed. Luciferasechannelrhodopsin communication is modeled and its performance is numerically validated, reporting on its throughput, bit error rate, propagation delay and energy consumption. IntroductionWith the current rapid development of technology, electronic and mechanical devices can be much smaller than even a decade ago, at the same time gaining new operating functionalities. However, when the device scale goes to the size of single molecules, cooperation between such devices and contacting them from outside their networks become quite challenging issues. Fӧrster Resonance Energy Transfer (FRET), a phenomenon characterized by very small propagation delays and a good throughput, has already been proposed for nanocommunications [1] and has been proved to be an efficient mechanism for transferring data at nanodistances [2][3][4]. Research on FRET for nanocommunication purposes and its integration with other elements on nanoworld is though at an early stage.The main contribution of the paper is solving two issues that, until now, were not solved for FRETbased communications [5]. The first issue is concerned with FRET signal storage. A molecule receiving a FRET signal cannot hold it and the signal must quickly be released, usually emitting a photon. Here, we propose to use channelrhodopsins which are molecules that can be used as nanoconverters, changing FRET signals into a voltage which can be detected by other devices. The second issue concerns FRET signal generation. In FRET nanocommunication experiments so far, a FRET transmission was initiated by an external laser source, which is rather troublesome inside a human body. Instead, we propose bioluminescent luciferase molecules as FRET transmitters, which are able to generate FRET signals themselves, taking energy from chemical reactions. We further discuss medical in-body systems where such FRET-based communication between luciferases and channelrhodopsins might be applied. We consider a system of small vesicles, liposomes, circulating through human vascular system and collecting data about body condition. The data gathered by liposomes are delivered to a detector placed in a human vein. Data transmission is realized by a luciferase-channelrhodopsin FRET interface. We model layers of luciferase and channelrhodopsin molecules based on their molecular structures and then we use their spectral characteristics to assess the e...

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“…Promoter expression has been monitored during cell growth, and the variable luciferase activities detected, demonstrating how certain genes are expressed in the gastrointestinal tract [ 66 ]. As an example of the potential of the technique, biosensors could be used to measure antibodies, enzymes, tumor necrosis factor or proteins of interest [ 67 , 68 , 69 , 70 ].…”

Section: Lab and Bifidobacteria As Biosensorsmentioning

confidence: 99%

Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors

Landete

Arqués

2017

IJMS

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Control and quantification of effector molecules such as heavy metals, toxins or other target molecules is of great biotechnological, social and economic interest. Microorganisms have regulatory proteins that recognize and modify the gene expression in the presence or absence of these compounds (effector molecules) by means of binding to gene sequences. The association of these recognizing gene sequences to reporter genes will allow the detection of effector molecules of interest with high sensitivity. Once investigators have these two elements—recognizing gene sequences and reporter genes that emit signals—we need a suitable vehicle to introduce both elements. Here, we suggest lactic acid bacteria (LAB) and bifidobacteria as promising carrier microorganisms for these molecular biosensors. The use of fluorescent proteins as well as food-grade vectors and clustered regularly interspaced short palindromic repeats (CRISPR) are indispensable tools for introducing biosensors into these microorganisms. The use of these LAB and bifidobacteria would be of special interest for studying the intestinal environment or other complex ecosystems. The great variety of species adapted to many environments, as well as the possibility of applying several protocols for their transformation with recognizing gene sequences and reporter genes are considerable advantages. Finally, an effort must be made to find recognizable gene sequences.

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Electrochemical impedance spectroscopy for black lipid membranes fused with channel protein supported on solid-state nanopore

Cited by 38 publications

References 52 publications

Lipid Bilayer Membrane in a Silicon Based Micron Sized Cavity Accessed by Atomic Force Microscopy and Electrochemical Impedance Spectroscopy

Lipid Bilayer Membrane in a Silicon Based Micron Sized Cavity Accessed by Atomic Force Microscopy and Electrochemical Impedance Spectroscopy

Signal generation and storage in FRET-based nanocommunications

Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors

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