Electrochemical magnetic microbeads-based biosensor for point-of-care serodiagnosis of infectious diseases

Cortina, María Eugenia; Melli, Luciano Jorge; Roberti, Mariano; Mass, Mijal; Longinotti, Gloria; Tropea, Salvador; Lloret, Paulina; Serantes, Diego A. Rey; Salomón, Francisco; Lloret, Matías; Caillava, Ana J.; Restuccia, Sabrina; Altcheh, Jaime; Buscaglia, Carlos A.; Malatto, Laura; Ugalde, Juan Esteban; Fraigi, Liliana; Moina, Carlos; Ybarra, Gabriel; Ciocchini, Andrés E.; Comerci, Diego J.

doi:10.1016/j.bios.2016.01.021

Cited by 73 publications

(55 citation statements)

References 19 publications

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“…As a first step towards the development of alternative PoC devices for diagnosis, a new diagnostic platform based on superparamagnetic microbeads coated with recombinant antigens and fluorescent (FMBIA) or electrochemical (EMBIA) detection was recently developed (Cortina et al, 2016). The EMBIA platform, including antigen-functionalized magnetic microbeads, disposable electrochemical cells-electrode cartridges and a portable potentiostat, was evaluated for serodiagnosis of human and cattle infectious diseases.…”

Section: Diagnostic Applications For Chagas D Isease: Pending Issuesmentioning

confidence: 99%

“…The EMBIA platform, including antigen-functionalized magnetic microbeads, disposable electrochemical cells-electrode cartridges and a portable potentiostat, was evaluated for serodiagnosis of human and cattle infectious diseases. In the particular case of Chagas disease, a more extensive validation was performed showing that the EMBIA platform displayed an excellent diagnostic performance almost indistinguishable from the well-established ELISA methods (Cortina et al, 2016). …”

Section: Diagnostic Applications For Chagas D Isease: Pending Issuesmentioning

confidence: 99%

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Chagas Disease Diagnostic Applications

Balouz

Agüero

Buscaglia

2017

Advances in Parasitology

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Chagas disease, caused by the protozoan Trypanosoma cruzi, is a life-long and debilitating illness of major significance throughout Latin America, and an emergent threat to global public health. Being a neglected disease, the vast majority of Chagasic patients have limited access to proper diagnosis and treatment, and there is only a marginal investment into R&D for drug and vaccine development. In this context, identification of novel biomarkers able to transcend the current limits of diagnostic methods surfaces as a main priority in Chagas disease applied research. The expectation is that these novel biomarkers will provide reliable, reproducible and accurate results irrespective of the genetic background, infecting parasite strain, stage of disease, and clinical-associated features of Chagasic populations. In addition, they should be able to address other still unmet diagnostic needs, including early detection of congenital T. cruzi transmission, rapid assessment of treatment efficiency or failure, indication/prediction of disease progression and direct parasite typification in clinical samples. The lack of access of poor and neglected populations to essential diagnostics also stress the necessity of developing new methods operational in Point-of-Care (PoC) settings. In summary, emergent diagnostic tests integrating these novel and tailored tools should provide a significant impact on the effectiveness of current intervention schemes and on the clinical management of Chagasic patients. In this chapter, we discuss the present knowledge and possible future steps in Chagas disease diagnostic applications, as well as the opportunity provided by recent advances in high-throughput methods for biomarker discovery.

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Section: Diagnostic Applications For Chagas D Isease: Pending Issuesmentioning

confidence: 99%

Section: Diagnostic Applications For Chagas D Isease: Pending Issuesmentioning

confidence: 99%

Chagas Disease Diagnostic Applications

Balouz

Agüero

Buscaglia

2017

Advances in Parasitology

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“…Disposable acrylic cartridges with eight electrochemical cells were used for the electrochemical measurements [19,20]. Each cell with a volume of 40 µL contained three screen printed electrodes: a carbon working electrode with an area of 1.6 mm 2 , a carbon counter electrode of approx.…”

Section: Optical and Electrochemical Measurementsmentioning

confidence: 99%

Optical and electrochemical detection of a verotoxigenic E. coli gene using DNAzyme-labeled stem-loops

Longinotti

Ybarra²,

Montserrat

2017

J. Electrochem. Sci. Eng.

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“…Mishra et al [10] reviewed various cancer biomarkers in saliva and compared the biomarkers efficacy with traditional diagnostics and state-of-the-art bioelectronics. Cortina et al [11] presented the development and validation of a portable, robust and inexpensive electrochemical magnetic biosensor.…”

Section: Introductionmentioning

confidence: 99%

Reliable Method for Steady-State Concentrations and Current over the Diagnostic Biosensor Transducers

Preethi¹,

Meena²,

Poovazhaki³

2017

AJAC

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A mathematical modelling of diagnostic biosensors system at three basic types of enzyme kinetics is discussed in the presence of diffusion. Enzyme kinetics is adopted to be first order, Michaelis-Menten and ping-pong mechanism. In this paper, approximate analytical solutions are obtained for the non-linear equations under steady-state conditions by using the new Homotopy perturbation method. Simple and closed forms of analytical expressions for concentrations of substrate, product and co-substrate and corresponding current response have been derived for all possible values of parameters. Furthermore, the numerical simulation of the problem is also reported here by using Matlab program. Good agreement between analytical and numerical results is noted.

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Electrochemical magnetic microbeads-based biosensor for point-of-care serodiagnosis of infectious diseases

Cited by 73 publications

References 19 publications

Chagas Disease Diagnostic Applications

Chagas Disease Diagnostic Applications

Optical and electrochemical detection of a verotoxigenic E. coli gene using DNAzyme-labeled stem-loops

Reliable Method for Steady-State Concentrations and Current over the Diagnostic Biosensor Transducers

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